Journal of Medical Sciences1682-44741812-5727Asian Network for Scientific Information10.3923/jms.2007.1098.1102 Lawsonia inermis and Strobilanthes crispus Extract Against Several Cancer Cell Lines]]>EndriniSusi RahmatAsmah IsmailPatimah Taufiq-YapY.H. 7200777This research was conducted to study and compare the cytotoxic effect of the extracts of two plants, henna (Lawsonia inermis) and kejibeling (Strobilanthes crispus) on several kinds of cancer cell lines. The mechanism of the effect was also studied through the expression of cancer-caused gene, c-myc. This research was done in vitro using several kinds of cancer cell lines such as human colon cancer cell lines (Caco-2), liver cancer cell lines (HepG2), hormone-dependent breast cancer cell lines (MCF-7) and hormone-independent breast cancer cell lines (MDA-MB-231) and Chang Liver cell lines. The cytotoxic effect was measured through MTT assay and the potential cytotoxic value was calculated by determining the toxic concentration which may kill up to 50% of the total cell used (IC50). Meanwhile, the cytotoxic mechanism was studied by determining the effect of adding both extracts to the c-myc gene expression. The methods for determination were RT-PCR and sequencing process. The results showed that chloroform extract from henna can be used against human colon cancer cell lines (Caco-2) and liver cancer cell lines (HepG2) with an IC50-value of 25.1 and 28 μg mL1, respectively. Kejibeling was also found to be cytotoxic against human liver cancer cell lines (HepG2) and hormone-dependent breast cancer cell lines (MCF-7) with an IC50-value of 0.3 and 24.8 μg mL1, respectively. However, the extract was not cytotoxic against human colon cancer cell lines (Caco-2). The smallest value of IC50 was seen when the kejibeling extract was compared with henna. The cytotoxic effect of both plant extracts may be mediated by the down-regulation of c-myc expression.]]>Alia, B.H., A.K. Bashir and M.O.M. Tanira,1995Lawsonia inermis L. (henna) in rats.]]>51356363Ali, A.M., M.M. Mackeen, I. Intan-Safinar, M. Hamid, N.H. Lajis, S.H. El-Sharkawy and M. Murakoshi,1996Juniperus chinensis.]]>53165169Alnemri, E.S., T.F. Fernandes, S. Haldar, C.M. Croce and G. Litwack,199252491495Balaji, K.C., H. Koul, S. Mitra, C. Maramag and P. Reddy et al.,1997c-myc antisense oligonucleotide in prostate cancer cells: A novel therapy in prostate cancer.]]>5010071015Brummitt, R.K. and C.E. Powell,19921st Edn.,Rostkowska, H., M.J. Nowak, L. Lapinski and L. Adamowicz,19985410911103Jiang, J.J., V. Slivova and D. Sliva,200629695703Kamei, H., T. Koide, T. Kojima, Y. Hashimoto and M. Hasegawa,199813185188Kusumoto, I.T., I. Shimada, N. Kakiuchi, M. Hattori, T. Namba and S. Supriyatna,19926241244Zakaria, M. and M.A. Mohd,1994Oritani, K., T. Kaisho, K. Nakajima and T. Hirano,19928022982305Rout, G.R., G. Das, S. Samantaray and P. Das,2001In vitro micropropagation of Lawsonia inermis (Lythraceae).]]>49957963Saito, H., T. Morizane, T. Watanabe, T. Kagawa, S. Miyaguchi, Kumagai and M. Tsuchiya,199148291296Sharma, K.,1990Lawsonia inermis Linn).]]>71293295Sunarto, P.A.,19771st Edn.,pp: 95-99pp: 95-99Endrini, S., A. Rahmat, P. Ismail and T.Y. Yun Hin,2002Lawsonia inermis).]]>2194197Vaidyaratnam, P.S.,19953rd Edn.,pp: 303-304pp: 303-304Van-Waardenburg, R.C., C. Meijer, H. Burger, K. Nooter, E.G. De-Vries, N.H. Mulder and S. De-Jong,199773544550Wall, M.E., H. Taylor and M.C. Wani,198750764766Woo, H.J. and Y.H. Choi,20052610171023