Journal of Medical Sciences1682-44741812-5727Asian Network for Scientific Information10.3923/jms.2006.984.988Campylobacters in Poultries` Faeces by PCR and its Comparison with Culture in Zahedan (Iran)]]>MohammadBokaeian Fard Amir H.Mohagheghi 6200666The present study was performed in order to identify the organisms in poultries by culture and the PCR. This is the first step in an effort to devise strategies for control of human Campylobacters infection in Zanhedan. One hundred and sixteen samples of rectal swabs from chickens of Zahedan (south-east Iran) poultry farms were collected and cultured on Campylobacter selective agar. Subsequently, identification was carried out by biochemical tests. DNA was isolated from suspensions, which were already prepared from stool samples and purified with specific kits. The PCR was done by application of species-specific primers of C. jejuni and C. coli. The results of culture in case of 11 samples were positive out of them 8 were as C. jejuni and 3 belonged to C. Coli, whereas in case of the PCR results 27 samples were positive out of them 18 isolates belonged to C. jejuni and 9 were found as C. coli. Hence, sensitivity and specificity of the PCR were found as 100 and 84.7%, respectively, therefore it is concluded that the PCR protocol applied in this study could identify all positive samples. Furthermore, detection of the species by the PCR was exactly identical with biochemical procedures, so taking into account accuracy and rapidity of the technique, it does seem that PCR is a reliable alternative for identification of Campylobacters in stool samples of the poultries.]]>Bokaeian, M. and M.M. Sultandallal,1995244554Bokaeian, M. and M. Dabirzadeh,199912530Coker, A.O., R.D. Isokpehi, B.N. Thomas, K.O. Amisu and C.L. Obi,20028237244Deming, M.S., R.V. Tauxe, P.A. Blake, S.E. Dixon and T.S. Jones et al.,1987Campylobacter enteritis at a university transmission from eating chicken and from cats.]]>126526534D'Sullivan, N.A., R. Fallon, C. Carrol, T. Smith and M. Maher,2000Campylobacter jejuni and Campylobacter coli in broiler chicken samples using a PCR/DNA probe membrane based colorimetric detection assay.]]>14716Eyigor, A., K.A. Dawson, B.E. Langlois and C.L. Pickett,1999Campylobacter species isolated from chicken arcasses.]]>6515011505Gonzalez, I., K.A. Grant, P.T. Richardson, S.F. Park and M.D. Collins,1997Campylobacter jejuni and Campylobacter coli by using a PCR test based on the ceuE gene encoding a putative virulence determinant.]]>35759763Goossens, H., M. Boeck, H. Coignau, L. Vlaes, C. Van den Borre and J.P. Butzler,1984Campylobacter spp. from feces: Comparison with Preston medium, a blood-free medium and a filtration system.]]>24840843Inglis, G.D. and L.D. Kalischuk,2003Campylobacter species in bovine feces.]]>6934353447Magistrado, P.A., M.M. Garcia and A.K. Raymundo,2001Campylobacter jejuni and Campylobacter coli from poultry in the Philippines.]]>70197206Mahendru, M., K.N. Prasad, T.N. Dhole and A. Ayyagari,1997Campylobacter jejuni strains by polymerase chain reaction and their restriction fragment length polymorphism analysis.]]>105914Meeyam, T., P. Padungtod and J.B. Kaneene,2004Campylobacter isolated from chickens and humans in Northern Thailand.]]>35670675Ng, L.K., C.I. Kingombe, W. Yan, D.E. Taylor, K. Hiratsuka and M.M. Garcia,1997Campylobacter jejuni by DNA hybridization and PCR.]]>6345584563Oberhelman, R.A. and D.N. Taylor,2000Campylobacter Infections in Developing Countries.]]>2000pp: 139-153pp: 139-153Pearce, R.A., F.M. Wallace, J.E. Call, R.L. Dudley and A. Oser et al.,2003Campylobacter within a swine slaughter and processing facility.]]>6615501556Pearson, A.D., M.H. Greenwood, J. Donalson, T.D. Healing and D.M. Jones et al.,200063309314Sacks, J.J., S. Lieb, L.M. Baldy, S. Berta and C.M. Patton et al.,198676424428Skirrow, M.B.,1991Campylobacter enteritis.]]>12916Stern, N.J.,1992Campylobacter jejuni and Approaches for Intervention in Poultry.]]>1992pp: 49-60pp: 49-60Tokumaru, M., H. Konuma, M. Umesako, S. Konno and K. Shinagawa,1991Salmonella and Campylobacter in meats in response to the sample size and the infection level of each species.]]>134146Van Looveren, M., G. Daube, L. de Zutter, J.M. Dumont and C. Lammens et al.,2001Campylobacter strains isolated from food animals in Belgium.]]>48235240Workman, S.N., G.E. Mathison and M.C. Lavoie,2005Campylobacter species in Barbados.]]>4326422650