Journal of Medical Sciences1682-44741812-5727Asian Network for Scientific Information10.3923/jms.2005.169.176Tamarindus indica Pulp]]>Garci TamayoFernando IzquierdoTeresa ReyesGuadalupe ElizaldePatricia RojasElba del CastilloEmilio Rojas TerrazasLuis I. 3200553A water-soluble Polysaccharide Extract (PE) isolated from the pulp of the fruit bodies of a Mexican species of Tamarindus indica L. was characterized by thin-layer chromatography and HPLC. Fructose, glucose, arabinose and xylose were the major components of the hydrolyzed PE samples. Normal lymphocytes and several lineages of malignant cells were cultured in presence of different amounts of PE solution and their effects on cellular proliferation were assessed by tritiated thymidine uptake. After incubation for 48 h, a significant reduction on the thymidine uptake was observed when Con-A-stimulated mices splenocytes were incubated with tamarind PE. Furthermore, a similar reduction in proliferative activity was detected also in CTLL-2 and HeLa cell lines. A comparative control assay showed a more intense inhibition when similar amounts (5-100 μg mL-1) of cis-platinum was added to HeLa cells. The morphological examination of PE-treated HeLa cells revealed their transformation into multinucleated giant cells. The results suggest that polysaccharide extracts obtained from tamarind pulp may contain active inhibitors of in vitro cellular multiplication.]]>Ishola, M.M., E.B. Agbaji and A.S. Agbaji,1990Tamarindus indica (Tsamiya) fruits grown in Nigeria.]]>51141143Marangoni, A., I. Alli and S. Kermasha,1988Tamarindus indica.]]>5314521455Rimbau, V., C. Cerdan, R. Vila and J. Iglesias,199913128132Khurana, A.L. and C.T. Ho,1989Tamarindus indica L.).]]>12419430Bhattacharya, S., S. Bal, R.K. Mukherjee and S. Bhattacharya,1994Tamarindus indica) kernel protein.]]>4919Lanhers, M.C., J. Fleurentin and F. Guillemni,1996Tamarindus indica L.]]>184257Mustapha, A., I.A. Yakasai and I.A. Aguye,1996Tamarindus indica L. on the bioavailability of aspirin in healthy human volunteers.]]>21223226Ali, M.S., V.U. Ahmad, I. Azhar and K. Usmanghani,1998Tamarindus indica.]]>694346Tsuda, T., M. Watanabe, K. Ohshima, A. Yamamoto, S. Kawakishi and T. Osawa,1994Tamarindus indica L.).]]>4226712674Sreelekha, T.T., T. Vijayakumar, R. Ankanthil, K.K. Vijayan and M.K. Nair,1993Tamarindus indica.]]>4209212Bradford, M.M.,197672248254Garcia-Tamayo, F., N. Malpica-Lopez, M. Aguirre and L.I. Terrazas-Valdes,1999in vitro proliferative response of zinc treated stressed mice's thymic lymphocytes.]]>77787Barclay, A.S. and R.E. Perdue,19766010811085Cordell, G.A., C.W.W. Beecher and J.M. Pezzuto,199132117221Hishida, Y., H. Nanba and H. Koruda,1988Grifola frondosa (Maitake).]]>3618191827Yoshizawa, Y., A. Ametani, J. Tsunehiro, K. Nomura, M. Itoh, F. Fukui and S. Kaminogawa,1995Prophyra yezoensis): Structure-function relationships and improved solubility.]]>5919331937Kiyohara, H., T. Matsumoto, N. Takemoto, H. Kawamura, Y. Komatsu and H. Yamada,199561429434Kambo, S.S., S. Shangary, J. Shing, K.K. Kambo and R.S. Sandhu,1995Araceae.]]>24845855Han, S.B., Y.H. Kim, C.W. Lee, S.M. Park and H.Y. Lee et al.,1998Angelica gigas Nakai.]]>403948Ng, T.B.,1998Coriolus versicolor (Basidiomycetes: Polyporaceae).]]>3014Berovic, M., J. Habijanic, I. Zore, B. Wraber, D. Hodzar, B. Boh and F. Pohleven,2003Ganoderma lucidum biomass and immunostimulatory effects of fungal polysaccharides.]]>1037786Ando, I., Y. Tsukumo, T. Wakabayashi, S. Akashi, K. Miyake, T. Kataoka and K. Nagai,2002211551162Lemassu, A. and M. Daffe,1994Mycobacterium tuberculosis.]]>297351357Kobayashi, A., M.I. Adenan, S.I. Kajiyama, H. Kanzaki and K. Kawazu,1996Tamarindus indica, Di-n-butyl malate and the structure activity relationship of its analogues.]]>51233242Zhuang, C., H. Itoh, T. Mizuno and H. Ito,1995Umitoranoo (Sargassum thunbergii).]]>59563567Miranda, E.I., C. Santana, E. Rojas, S. Hernandez, P. Ostrosky and A. Garcia-Carranca,1996349173182Rodilla, V.,1993300281291Sodhi, A.,197917623627Tounekti, O., G. Pron, J. Belehradek and L.M. Mir,19935354625469Lock, R.B., O.V. Galperina, R.C. Feldhoff and L.J. Rhodes,19945449334939Peyrot, V., C. Briand, R. Momburg and J.C. Sari,1986In vitro mechanism study of microtubule assembly inhibition by cis-dichlorodiammine-platinum (II).]]>35371375