Journal of Medical Sciences1682-44741812-5727Asian Network for Scientific Information10.3923/jms.2004.132.135KazemiB. .Bandehpour zadehH. Yahya RoozbehiM. SeyedN. GhotaslooA. TaherpourA. 2200442HCV is one of the main causes of chronic hepatitis in developing countries
and is particularly distributed via blood and blood product transfusion. ELISA
serological method is currently used as laboratory diagnostic test in Iran,
but both clinical and laboratory diagnosis of acute HCV infection remains yet
as a problem. The aim of this study was to determine results obtained by PCR
and ELISA techniques in detection of HCV in serum samples. Using ELISA and RT-PCR,
159 serum samples were analyzed. Thirty four negative samples (22.35%) and 45
positive samples (27.95%) were detected by both techniques. Thirty two samples
(19.88%) were positive by PCR and negative by ELISA and 48 samples (29.82%)
were negative by PCR and positive by ELISA. Present results show that there
are significant differences between PCR and ELISA diagnostic tests for HCV detection.]]>Iancu, L.S.,20011053742Schmidt, W.N., P. Wu, J.Q. Han, M.J. Perino, D.R. La Brecque and J.T. Stapleton,19971762026Gitnik, G.,19985826570Colombo, M.,1998154337344Park, Y.S., K.O. Lee, M.J. Oh and Y.G. Chai,199847643647Roggendorf, M., M. Lu, H. Meisel, M. Riffelmann, E. Schreier and S. Viazov,1996242634Kuo, G., Q.L. Choo, H.J. Alter, G.L. Gitnick and A.G. Redeker et al.,1989244362364Fanci, P.A. and D.C. Wong,199132598104Dow, B.,19991391104Weiner, A.J., G. Kuo, D.W. Bradley, F. Bonino and G. Saracco et al.,199033513Yokosuka, O., F. Hiroshige, M. Imazek, H. Tagawa, S. Sisho and M. Omata,199831394399Newton, C.R. and A. Graham,19972nd Edn.,pp: 63-74pp: 63-74Sambrook, J. and D.W. Russell,2001pp: 4-14pp: 4-14Marian, E.B., M. Rehermann and S.M. Feinstone,20014th Edn.,pp: 1127-1161pp: 1127-1161