Journal of Medical Sciences1682-44741812-5727Asian Network for Scientific Information10.3923/jms.2002.29.31+, K+-ATPase from Different Mice Brain Lobes]]>KumosaniT. A. 1200221The kinetic properties of Na+, K+-ATPase from different lobes of swiss albino
mice brain were studied under conditions of enzyme saturation with Mg-ATP
(0.25-10 mM). By participating in the regulation of ion and voltage gradients, the
Na-K pump (Na+, K+-ATPase) influences many aspects of cellular physiology.
The apparent Michaelis constant (Km) (Mm) and the apparent maximum velocity
(Vmax) (n mol Pi min-1 mg protein) for mice brain lobes Bl-B5 were as follows
(0.57; 616), (1.45; 848), (8.44; 7634), (3.19; 95) and (3.34; 110), respectively.
The specific activity of lobe B3 has the highest followed by B2, B1, B5 and B4 in
decreasing order of activity. It seems that each brain lobe has its own Michaelis
constant (Km) and maximum velocity (Vmax) that is needed for their own
physiological role. Differences in kinetic properties of Na, K-ATPase from
different brain lobes may be due to the isoenzyme diversity and adaptations to
specific physiological demands of the specific brain regions.]]>Raheem, K.A., E. Grunding, F. Salvenmoser, R. Schedl and J. Weis,197914C) glucose into metabolites of brain, liver and blood of rats pretreated with reserpine or phenothiazines.]]>17341348Averet, N., E. Arrigoni, H. Loiseau and F. Cohadon,1987+-K+-ATPase activity of glial, neuronal, and synaptosomal enriched fractions from normal and freezing-injured rabbit cerebral cortex.]]>12607612Beauge, L.A., D.C. Gadsby and P.J. Garrahan,1997pp: 1-2pp: 1-2Boldyrev, A.A., N.U. Fedosova and O.D. Lopina,1991+, K+-ATPase.]]>2450454Brum, G., L. McKane and G. Karp,19942nd Edn.,Fiske, C.H. and Y. Subbarow,192566375400Ganea, C., A. Babes, C. Lupfert, E. Grell, K. Fendler and R.J. Clarke,1999+, K+-ATPase.]]>77267281Hylland, P., S. Milton, M. Pek, G.E. Nilsson and P.L. Lutz,1997+, K+-ATPase activity in two anoxia tolerant vertebrates: Crucian carp and freshwater turtle.]]>2358992Lowry, O.H., N.J. Rosebrough, A.L. Farr and R.J. Randall,1951193265275Lopina, O.D., N.A. Sarvazyan, A. Askari and A.A. Boldyrev,1995+/K+-ATPases of duck salt gland and canine kidney: Implications for the enzyme′s reaction mechanism.]]>321429433Pekovic, S., N. Nedeljkovic, G. Nikezic, A. Horvat, M. Stojiljkovic, L. Rakic and J.V. Martinovic,199716227240Serrano, R., B.I. Kanner and E. Racker,197625124532461Stahl, W.L.,19868449476Wyse, A.T.S., M. Wajner and M.D.W. Davis,1998+, K+-ATPase activity by phenylalanine and phenyllactate in the synaptic plasma membrane from the cerebral cortex of rats.]]>26141143Wu, P.H.,1986+, K +-ATPase in Nervous Tissue.]]>1986