Journal of Medical Sciences1682-44741812-5727Asian Network for Scientific Information10.3923/jms.2001.251.254A. MahmodAbdullah 4200114The Effect of mercury chloride (HgCl2) on the human plasma cholinesterase has
been investigated. Hg+2 was very effective inhibitor producing 50% inhibition at
1.2 x 10‾6 M and nearly 100% inhibition at 0.05 x 10‾6 M. The study of initial
velocity indicates a noncompetitive mechanism of inhibition by Hg+2 when
butrylthiocholine (BTCh) is substrate. Inhibition shows a Ki of 0.25µM. But when
ATCh is substrate the inhibition was uncompetitive. The secondary plots of
inhibition with respect to ATCh indicates a dissociation constant (Ki) of 0.12µM.
The inhibition was dependent on the substrate employed during the activity assay.
In conclusion, the distinct behavior of butrylcholinesterase(BChE) for Hg+2 may
reflect the Subtle differences in the amino acids which provide the environment
when the ‘catalytic tried’ is located.]]>Abdelsalam, E.B.,198729146148Abdullah, A.M.,200054147Abdullah, A.M.,20012858Baes, C.F.Jr. and R.E. Mesmer,1976pp: 104-111pp: 104-111Balasubramanian, A.S. and C.D. Bhanumathy,1993713541358Bhanumathy, C.D. and A.S. Balasubramanian,199830695705Chatonnet, A. and O. Lockridge,1989260625634Ellman, G.L., K.D. Courtney, V. Andres Jr. and R.M. Featherstone,196178890Garry, P.J. and J.I. Routh,1965119196Hill, E.F. and J.H. Soares Jr.,198720105116Kalow, W. and D.R. Gunn,1957120203214Kamikashi, T., K. Kizaki, K. Murakami and S. Ishibashi,1974137139142Kambam, J.R., S.M. Perry, S. Enthman and B.E. Smith,1988159309311Lepage, L., F. Schiele, R. Gueguen and G. Siest,198531546550Lockridge, O.,19889125128Mounter, L.A., H.C. Alexander, K.D. Tuck and L.T.H. Dien,1957226867872Marquis, J.K. and A.J. Lerrick,1982Electrophorus electricus.]]>3114371440Masson, P., M.T. Froment, C.F. Bartels and O. Lockridge,19962353648Segel, I.R.,1975Sussman, J.L., M. Harel, F. Frolow. C. Oefner, A. Tokler and I. Silman,1991Torpedo californica: A prototypic acetylcholine-binding protein.]]>253872879Yamazaki, S., Y. Dokia, T. Watanabe and K. Fuwa,1978218Lineweaver, H. and D. Burk,193456658666Leatherbarrow, R.J. and G. Fit,1992Graphics Program for the IBM PC.]]>Version 3.0,