Research Journal of Microbiology1816-4935xxxx-xxxxScience International10.3923/jm.2021.1.7mecA and sea genes on Staphylococcus aureus using Quantitative PCR Assay]]>OjoS.K.S. EsumehF.I. SunmonuG.T. 12021161Background and Objective: Methicillin Resistant Staphylococcus aureus (MRSA)is one of the major causes of nosocomial infections and are most profound in community in previously healthy individuals. To detect and quantify antibiotic resistant and virulence genes present in methicillin sensitive S. aureus (MSSA) strains from wounds and burns patients. Materials and Methods: About 200 clinical samples were obtained for S. aureus isolation, identified and characterized by using standard microbiological procedures. Methicillin resistance was determined by using β-lactamase assay and oxacillin disk (Oxoid) susceptibility test. Quantification of the S. aureus strains was performed using quantitative Polymerase Chain Reaction (qPCR) assay. Agarose gel electrophoresis was carried out on the qPCR products using 1.5% agarose gel with a standard DNA ladder (100 bp), visualized under UV transilluminator and the image taken using digital camera. Results: Almost 44 (22%) S. aureus were isolated and characterized with 36 (82%) strains producing β-lactamase and were resistant to oxacillin (MRSA) while, 8 (18%) strains do not produce β-lactamase and were sensitive to oxacillin (MSSA). The β-lactamase and non-β-lactamase isolates were resistant to other antibiotics. The quantification of PCR products indicated that sea genes (virulence enterotoxin factor) were detected from the antibiotic resistant staphylococci ranging from 0-13551.84 nmoles while, the quantification of mecA genes detected ranged from 0-2601.76 nmoles. The agarose gel electrophoresis of the PCR products of mecA and sea genes showed amplicon size of 657 bp for mecA and 526 bp for sea genes after amplification of the antibiotic resistant S. aureus strains. Conclusion: This study detected the presence of antibiotic resistant and virulence genes associated with MRSA in MSSA, which calls for urgent clinical and pharmaceutical attention.]]>Mansour, F.A., Y.A. El-Zawahry, M.A. Abouelela and M.M. Khashaba,2009Staphylococcus aureus strains responsible for wound infections and septicemia in Mansoura university hospitals.]]>55965Naik, D. and A. Teclu,2009Staphylococcus aureus in Eritrea.]]>2009Sharma, N.K., R. Garg, S. Baliga and B.K. Gopalkrishna,2013Staphylococcus aureus.]]>721782180Craciunas, C., A. Butiuc-Keul, M. Flonta, A. Almas, A. Brad and M. Sigarteu,2010Staphylococcus aureus.]]>17248252Saadati, M., B. Barati, M. Doroudian, H. Shirzad and M. Hashemi et al.,2011Staphylococcus aureus isolated from nasal carriers in Tehran province, Iran, by multiplex pcr.]]>23440Ojo, S.K.S., T.O. Onyechi and F.I. Esumeh,201377074CLSI,20082008Jang, H.J., C. Nde, F. Toghrol and W.E. Bentley,2008Staphylococcus aureus.]]>2008Grisold, A.J., E. Leitner, G. Mühlbauer, E. Marth and H.H. Kessler,2002Staphylococcus aureus and simultaneous confirmation by automated nucleic acid extraction and real-time PCR.]]>4023922397Sambrook, J. and D.W. Russell,20013rd Edn.,Pages: 2344Pages: 2344Francois, P., D. Pittet, M. Bento, B. Pepey, P. Vaudaux, D. Lew and J. Schrenzel,2003Staphylococcus aureus directly from sterile or nonsterile clinical samples by a new molecular assay.]]>41254260Lovseth, A., S. Loncarevic and K.G. Berdal,20044238693872Sauer, P., J. Sila, T. Stosova, R. Vecerova and P. Hejnar et al.,2008Staphylococcus aureus isolates from the University Hospital, Olomouc, Czech Republic.]]>57403410Klotz, M., S. Opper, K. Heeg and S. Zimmermann,2003Staphylococcus aureus enterotoxins A to D by real-time fluorescence PCR assay.]]>4146834687Omoe, K., M. Ishikawa, Y. Shimoda, D.L. Hu, S. Ueda and K. Shinagawa,2002seg, seh and sei genes in Staphylococcus aureus isolates and determination of the enterotoxin productivities of S. aureus isolates harboring seg, seh, or sei genes.]]>40857862Mehrotra, M., G. Wang and W.M. Johnson,2000Staphylococcus aureus enterotoxins, exfoliative toxins, toxic shock syndrome toxin 1 and methicillin resistance.]]>3810321035Becker, K., A.W. Friedrich, G. Lubritz, M. Weilert, G. Peters and C. von Eiff,2003Staphylococcus aureus isolated from blood and nasal specimens.]]>4114341439Warren, D.K., R.S. Liao, L.R. Merz, M. Eveland and W.M. Dunne,2004Staphylococcus aureus directly from nasal swab specimens by a real-time PCR assay.]]>4255785581Al-Ruaily, M.A. and O.M. Khalil,2011mecA) gene in Methicillin Resistant Staphylococcus aureus (MRSA) at Prince A/Rhman Sidery Hospital, Al-Jouf, Saudi Arabia.]]>34145 Khan, A.K.S., P.J. Shetty, Y.L. Sarayu, A. Chidambaram and R. Ranganathan,2012Staphylococcus aureus by polymerase chain reaction.]]>16468Jonas, D., M. Speck, F.D. Daschner and H. Grundmann,2002Staphylococcus aureus from screening swabs.]]>4018211823Fosheim, G.E., A.C. Nicholson, V.S. Albrecht and B.M. Limbago,20114930713073