Research Journal of Microbiology1816-4935xxxx-xxxxScience International10.3923/jm.2020.15.21Penicillium chrysogenum Strains Isolated from Plectranthus amboinicus L. of West Malaysia]]>TiwariKartikeya GanesenManjuu 12020151Background and Objective: In nature, plants incorporate a wide scope of restoratively esteemed bioactive compounds. The fungal endophytes are the organisms which grow inside the plant parts such as roots, stems, leaves and flowers and produce similar bioactive compounds of medicinal significance. The fungal endophytes from the plant Plectranthus amboinicus were not well explored in West Malaysia. Therefore, this study was designed with the objectives to harness novel endophytic bioactive compound producing fungal strains from this plant. Materials and Methods: Screening for fungal endophytes was performed by using surface sterilisation method. Sterile explants were placed on a potato dextrose agar (PDA) plates and incubated under 28°C. These fungal endophytes were identified microscopically by lactophenol-cotton blue staining protocol and molecular characterization was performed by amplification of randomly amplified polymorphic DNA tool. Results: A total of ten fungal endophytes were isolated and identified. The prevalent endophytic fungal strain found was of Penicillium chrysogenum in comparison with Aspergillus niger, Fusarium oxysporum and Alternaria solani. Therefore, these strains selected for assessment of genetic diversity. Conclusion: The most prevalent bioactive compound producing fungal strain found was of Penicillium chrysogenum associated endophytically with the plant Plectranthus amboinicus.]]>Svara, F. and R.J. Rank,20112011Arnold, C.,20133821516Arumugam, G., M.K. Swamy and U.R. Sinniah,2016Plectranthus amboinicus (Lour.) Spreng: Botanical, phytochemical, pharmacological and nutritional significance.]]>2016Shoeb, M., M. Haque and N. Nahar,2014Penicillium thiomii isolated from Terminalia chebula Retz.]]>46570Toghueo, R.M.K. and F.F. Boyom,2019Terminalia species: A comprehensive review.]]>2019Maheshwari, V.L., M.P. Patil, R.H. Patil and S.G. Patil,2014Terminalia arjuna and their biological activities.]]>35361Rajeswari, S., R. Revathi and A.D. Prasanth,2017Terminalia chebula of Eastern Ghats, Tamilnadu.]]>102123Kim, C.S., M.S. Park and S.H. Yu,2008Penicillium from Pinus rigida in Korea.]]>36222227Verma, V.C., S.K. Gond, A. Kumar, R.N. Kharwar and G. Strobel,2007Azadirachta indica A. Juss (neem) from Varanasi (India).]]>54119125Tiwari, K.,2013Alternaria alternata accessions isolated from Pongamia pinnata L.]]>1610041009Tiwari, K. and M. Chittora,2013Alternaria solani isolates associated with the dominant Karanja plants in Sanganer region of Rajasthan.]]>2013Chen, Z.W., C.H. Ku, C.J. Weng and T.L. Chen,199767249258Rafi, M.,2001Penicillium chrysogenum.]]>2001Rahman, S.U., M.H. Rasool and M. Rafi,2012Penicillium chrysogenum in Pakistan.]]>43476481Guo, L.D., G.R. Huang, Y. Wang, W.H. He, W.H. Zheng and K.D. Hyde,2003Pinus tabulaeformis.]]>107680688Astuti, P., S. Sudarsono, K. Nisak and G.W. Nugroho,2014Coleus amboinicus lour exhibited antimicrobial activity.]]>4599605Gaikwad, M.N., S.S. Bodke and L.H. Kamble,2017Coleus aromaticus (Benth.).]]>26467Rajendran, L., K. Rajagopal, K. Subbarayan, K. Ulagappan, A. Sampath and G. Karthik,20131112121Korejo, F., A.S. Ali, A.H. Shafique, V. Sultana, J. Ara and S. Ehteshamul-Haque,2014Penicillium species isolated from Salvadora species.]]>4623132318Campos, P.R.C., K.J.S. Jacob, H.C. Ramos and F.B. Temanel,2019Plectranthus amboinicus Lour.) leaves.]]>8103110Sukrutha, S.K., Z. Adamechova, B.K. Rachana, S. Janakiraman and M. Certik,201214449Urooj, F., H. Farhat, S.A. Ali, M. Ahmed and V. Sultana et al.,2018Penicillium species in suppressing the root rotting fungi of sunflower.]]>5016211628Alves, D.R., S.M. de Morais, F. Tomiotto-Pellissier, F.R. Vasconcelos and F.D.C.O. Freire et al.,20182018Houbraken, J., J.C. Frisvad, K.A. Seifert, D.P. Overy, D.M. Tuthill, J.G. Valdez and R.A. Samson,2012Penicillium species and an overview of section Chrysogena.]]>2978100