Research Journal of Microbiology1816-4935xxxx-xxxxScience International10.3923/jm.2020.1.8Sparisoma viride and Bioencapsulation of Artemia salina with Probionts]]>YogananthN. AkilaM. AliM. Syed JayaprakashvelM. MuthezhilanR. 12020151Background and Objective: Now a days, the need for remarkable disease resistance, growth of aquatic organisms and feed competence has brought about the employ of probiotics in aquaculture field. The objective of the present study was to isolate probiotic bacterial strains from fish intestines and screen them by in vitro testing of their antagonism to pathogens and bioencapsulation efficacy. Materials and Methods: Six bacteria were isolated from marine fish gut and identified by biochemical test. All six isolates were assessed in vitro for its inhibitory activity against common fish pathogens of Aeromonas hydrophila and Vibrio harvey by well diffusion assay. Based on the antimicrobial activity, the Enterococcus, Streptococcus and Bacillus were selected for assaying probiotic properties of acid tolerance, bile salt concentration and auto aggregation assay. The 24 h old Artemia salina nauplii were selected for probiont encapsulation study. Results: The isolates Enterococcus, Streptococcus and Bacillus were confirmed as probionts by above mentioned three methods. The research of encapsulation studies exposed that the gut loading and evacuation time of Artemia nauplii differed according to the oils and probiotics used for the trials. Probiotics take less time for gut loading and retention when compared to oils. Conclusion: The findings of this study indicated that the indigenous fish gut bacteria provided benefit to the culture fishes in terms of pathogen protection and increasing growth potential and thus fulfilled the major requirements of being effective probiotics]]>Toranzo, A.E., B. Magarinos and J.L. Romalde,20052463761Austin, B. and X.H. Zhang,2006Vibrio harveyi: A significant pathogen of marine vertebrates and invertebrates.]]>43119124Paillard, C., F. Le Roux and J.J. Borrego,200417477498Aoki, T.,1975Plecoglossus altivelis).]]>19712Cabello, F.C.,2006811371144Zhou, Q., K. Li, X. Jun and L. Bo,200910037803786Kurath, G.,200827175196Dorrington, T. and M. Gomez-Chiarri,200827365374Kesarcodi-Watson, A., H. Kaspar, M.J. Lategan and L. Gibson,2008274114Guarner, F. and J.R. Malagelada,2003361512519Balcazar, J.L., I. de Blas, I. Ruiz-Zarzuela, D. Cunningham, D. Vendrell and J.L. Muzquiz,2006114173186Patra, B.C. and P. Bandyopadhyay,20022002pp: 220-225pp: 220-225Al-Faragi, J.K.H. and S.A.A. Alsaphar,2012Bacillus subtilus as probiotic from intestinal microflora of common carp Cyprinus carpio L.]]>36355361Perdigon, G., S. Alvarez, M. Rachid, G. Aguero and N. Gobbato,19957815971606Bhat, B.V.,1992Artemia aquabusiness in India.]]>242731Immanuel, G., A. Palavesam and M. Petermarian,2001Artemia nauplii on survival, growth, fatty acids and stress resistance of postlarvae Penaeus indicus.]]>14377388Krishnakumar, V.,2012Artemia sp. in solar salt fields off Kelambakkam, South India and impact of the biotic community on salt quality.]]>2012Divya, K.R., A. Isamma, V. Ramasubramanian, S. Sureshkumar and T.S. Arunjith,2012Puntius conchonius.]]>33551555Austin, B. and D.A. Austin,1987pp: 13-350pp: 13-350Jahangiri, L., M. Sudagar, O. Ashayerizadeh, H. Kolangi and A. Tabarraei,2018Poecilia reticulata (Cyprinodontiformes: Poecilidae).]]>18809815Bergh, O.,1995Hippoglossus hippoglossus L., inhibit growth of a pathogenic Vibrio sp.]]>183140Brunt, J. and B. Austin,2005Oncorhynchus mykiss (Walbaum).]]>28693701Hjelm, M., A. Riaza, F. Formoso, J. Melchiorsen and L. Gram,2004Roseobacter spp. and Vibrionaceae strains in a turbot larva (Scophthalmus maximus) rearing system.]]>7072887294Rengpipat, S., W. Phianphak, S. Piyatiratitivorakul and P. Menasveta,1998Penaeus monodon survival and growth.]]>167301313Cakir, I.,20032003Prasad, J., H. Gill, J. Smart and P.K. Gopal,1998Lactobacillus and Bifidobacterium strains for use as probiotics.]]>89931002Bujnakova, D. and V. Kmet,2002Escherichia coli.]]>49152154Schachtsiek, M., W.P. Hammes and C. Hertel,2004Lactobacillus coryniformis DSM 20001T surface protein Cpf mediating coaggregation with and aggregation among pathogens.]]>7070787085Citarasu, T., G. Immanuel and M.P. Marian,1998Artemia enriched with stresstol and cod liver oil on growth and stress resistance in the Indian white shrimp Penaeus indicus post larvae.]]>126575Smith, G.G., A.J. Ritar, C.F. Phleger, M.M. Nelson, B. Mooney, P.D. Nichols and P.R. Hart,2002Artemia after oil enrichment and during starvation.]]>208137158Narciso, L.,2000Artemia sp. (Crustacea, Branchiopoda): Its use in aquaculture.]]>2000