Research Journal of Microbiology1816-4935xxxx-xxxxScience International10.3923/jm.2018.127.137Montipora digitata]]>Duc NguyenThe CasaretoBeatriz Estela RamphulChitra ToyodaKeita SuzukiToshiyuki FujiwaraTaketomo SuzukiYoshimi 32018133Background and Objective: Little is known about glycerol as a mediator between zooxanthellae and coral bacterial communities. This study evaluated the amount of glycerol in the coral holobiont of M. digitata and its effects as growth enhancer and antimicrobial properties of two Vibrio bacteria isolated from M. digitata.Materials and Methods: The amount of glycerol in tissue of M. digitata was determined after four days exposure to temperature (27, 32°C) and an extended period of light (12 and 18 h per day). Next, culture-independent and agar diffusion techniques were used to assess the antagonistic/antimicrobial properties of potential Vibrio bacteria isolated from M. digitata. Effect of glycerol on growth and antimicrobial properties of two selected Vibrio bacteria were examined by liquid culture assays. Results: Seventy-one isolated colonies from M. digitata were tested against the pathogen V. coralliilyticus SWA07, only six (8.5%) showed antimicrobial activity. Results indicated that isolates MH66 and MB3 displayed antibacterial and antagonistic activity against a wide range of target bacteria. So these were selected for further study. Two coral bacterial isolates MH66 and MB3 were closely related to Vibrio sp. displayed potent antagonistic/antimicrobial activity against target bacteria. Glycerol concentrations (from 0.5 to 10 mg mL1) significantly enhanced the growth and antimicrobial properties of both MH66 and MB3. Conclusion: Glycerol concentration increased when M. digitata was exposed to light and temperature stresses and it enhanced the growth and antimicrobial properties of Vibrio MH66 and MB3.]]>Rosenberg, E., O. Koren, L. Reshef, R. 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