Research Journal of Microbiology1816-4935xxxx-xxxxScience International10.3923/jm.2018.100.118Staphylococcus aureus Strains Isolated from Clinical and Hospital Environment specimens and Their Correlation with PCR-based Approaches]]>TaechowisanThongchai MungchukeatsakulNutthapol S. PhutdhawongWaya 22018132Background and Objective:Staphylococcus aureus has long been recognized as one of the most important causes of bacterial disease in humans. This study investigates the prevalence of Staphylococcus aureus in clinical and environmental samples and their susceptibility patterns to antibiotics. Methodology: A total of 69 samples, 36 clinical specimens obtained from patients and 33 environmental samples from hospital facilities, were screened for S. aureus using standard microbiological and biochemical methods and PCR-based assay. Isolates resistant to both cefoxitin and oxacillin were considered to be Methicillin Resistant S. aureus (MRSA). Results: Of the two categories of samples screened, 31 (86.1%) and 23 (69.7%), respectively tested positive for S. aureus. The highest prevalence of MRSA from clinical samples (28.6%) was found in sputum and wounds and the highest (25%) from environmental samples was found in corridor, door handle and patient bed samples. Conclusion: In suggesting that healthcare personnel and hospital environments serve as potential reservoirs of S. aureus, these findings have practical, clinical and epidemiological importance. Ten of 16 of multi-drug resistant S. aureus isolates were MRSA, suggesting a correlation between the results of PCR patterns and their phenotypic multi-drug resistance testing.]]>Talan, D.A., A. Krishnadasan, R.J. Gorwitz, G.E. Fosheim, B. Limbago, V. Albrecht and G.J. Moran,2011Staphylococcus aureus from skin and soft-tissue infections in US emergency department patients, 2004 and 2008.]]>53144149Gleghorn, K., E. Grimshaw and E. Kelly,20152079Cosgrove, S.E., G. Sakoulas, E.N. 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