Research Journal of Microbiology1816-4935xxxx-xxxxScience International10.3923/jm.2017.20.32HardingM.W. ButlerN. DmytriwW. RajputS. BurkeD.A. HowardR.J. 12017121Background and Objective: Microorganisms can colonize or contaminate fruits and vegetables at virtually any point during production,
distribution, processing, packaging or food preparation. Microbial contamination of fruits and vegetables can cause food spoilage and
human infections. This study was undertaken to collect, isolate and identify commonly occurring food spoilage microorganisms from
a wide variety of fresh, direct-market fruits and vegetable in Southern Alberta and to test for the presence of the food-borne pathogen
Listeria monocytogenes on a few representative farm operation surfaces. Methodology: The naturally-occurring microorganisms present
on produce were amplified by incubation in humid chambers. Microorganism cultures were obtained by aseptically isolating from produce
surfaces and sub-culturing on agar petri dishes. Results: Approximately 950 microbial isolates, both bacteria and fungi were collected
from fresh produce. Identities of 80 selected isolates were confirmed using molecular analysis. A number of well-known plant
pathogenic taxa were identified along with a few species that have not previously been reported to act as spoilage organisms, namely
Isaria farinosus, Stenotrophomonas maltophilia, Rahnella aquatilis and Acinetobacter calcoaceticus (syn. Micrococcus calcoaceticus ).
Listeria monocytogenes was not detected at any of the Southern Alberta farms sampled. Conclusion: These results indicate that a wide
array of microorganisms are capable of causing food spoilage and some have not yet been documented or characterized.]]>Critzer, F.J. and M.P. Doyle,201021125130Leff, J.W. and N. Fierer,20132013Liao, C.H. and W.F. Fett,20016411101115Round, J.L. and S.K. Mazmanian,20099313323Lee, Y.K. and S.K. Mazmanian,201033017681773Tournas, V.H.,2005313344Hanski, I., L. von Hertzen, N. Fyhrquist, K. Koskinen and K. Torppa et al.,201210983348339King, A.D., J.A. Magnuson, T. Torok and N. Goodman,199156459461Nguyen-The, C. and F. Carlin,199434371401Beuchat, L.R.,199659204216Badosa, E., R. Trias, D. Pares, M. Pla and E. Montesinos,200888605611Ponce, A.G., M.V. Aguero, S.I. Roura, C.E. Del Valle and M.R. Moreira,200873M257M263Oliveira, M., J. Usall, I. Vinas, M. Anguera, F. Gatius and M. Abadias,201027679684Rastogi, G., A. Sbodio, J.J. Tech, T.V. Suslow, G.L. Coaker and J.H. Leveau,2012618121822Liu, N.T., A.M. Lefcourt, X. Nou, D.R. Shelton, G. Zhang and Y.M. Lo,201376827832Shapaval, V., J. Schmitt, T. Moretro, H.P. Suso and I. Skaar et al.,2013114788796Liu, N.T., X. Nou, A.M. Lefcourt, D.R. Shelton and Y.M. Lo,2014Escherichia coli O157:H7 and environmental bacteria isolated from fresh-cut processing facilities.]]>1711520Donlan, R.M.,20028881890Hall-Stoodley, L., J.W. Costerton and P. Stoodley,2004295108Harding, M.W., L.L.R. Marques, R.J. Howard and M.E. Olson,200917475480Fett, W.F. and P.H. Cooke,2003494550Heaton, J.C. and K. Jones,2008104613626Berger, C.N., S.V. Sodha, R.K. Shaw, P.M. Griffin, D. Pink, P. Hand and G. Frankel,20101223852397Jahid, I.K. and S.D. Ha,2014139901011Marques, L.L.R., S.H. De Boer, H. Ceri, E. Lee and M.E. Olson,2003Erwinia carotovora biofilms to antibiotics.]]>93S57S57Harding, M.W., L.L. Marques, R.J. Howard and M.E. Olson,201044347Pirie, J.H.H.,1940145264264Murray, E.G.D., R.A. Webb and M.B.R. Swann,1926Bacterium monocytogenes (n.sp.).]]>29407439Bohaychuk, V.M., R.W. Bradbury, R. Dimock, M. Fehr and G.E. Gensler et al.,200972415420Jagoueix, S., M.J. Bove and M. Garnier,199444379386Kaneshiro, W.S., C.Y. Mizumoto and A.M. Alvarez,2006Clavibacter michiganensis subsp. michiganensis from seed-borne saprophytes using ELISA, Biolog and 16S rDNA sequencing.]]>1164556Altschul, S.F., T.L. Madden, A.A. Schaffer, J. Zhang, Z. Zhang, W. Miller and D.J. Lipman,19972533893402Coates, D. and J.E. Death,197831148152Yashiro, E., R.N. Spear and P.S. McManus,201111012841296Palleroni, N.J. and J.F. Bradbury,1993Xanthomonas maltophilia (Hugh 1980) Swings et al. 1983.]]>43606609Izard, D., F. Gavini, P.A. Trinel and H. Leclere,1979Rahnella aquatilis, a new member of the enterobacteriaceae].]]>130163177Calvo, J., V. Calvente, M.E. de Orellano, D. Benuzzi and M.I.S. de Tosetti,2007Penicillium expansum and Botrytis cinerea in apple by using the bacterium Rahnella aquatilis.]]>113251257Zimmermann, G.,2008Isaria farinosa (formerly Paecilomyces farinosus) and the Isaria fumosorosea species complex (formerly Paecilomyces fumosoroseus): Biology, ecology and use in biological control.]]>18865901Hayward, A.C., N. Fegan, M. Fegan and G.R. Stirling,2010108756770Giobbe, S., S. Marceddu, B. Scherm, G. Zara, V.L. Mazzarello, M. Budroni and Q. Migheli,2007Pichia fermentans, which controls Monilinia brown rot on apple but is pathogenic on peach fruit.]]>713891398Kumar, C.G. and S.K. Anand,199842927Korber, D.R., A.K. Mangalappalli-Illathu and S. Vidovic,20092009pp: 169-199pp: 169-199Simoes, L.C., M. Simoes and M.J. Vieira,20107666736679Van Meervenne, E., R. De Weirdt, E. Van Coillie, F. Devlieghere, L. Herman and N. Boon,201470332338