Research Journal of Microbiology1816-4935xxxx-xxxxScience International10.3923/jm.2016.11.19Staphylococcus aureus and Coagulase Negative Staphylococci Isolates from Mastitis Milk of Etawa Crossbred Goat]]>WindriaSarasati WidianingrumDesy Cahya SalasiaSiti Isrina Oktavia 12016111This study aimed to identify staphylococcal mastitis milk in Peranakan Etawa (PE) or Etawa crossbred goats in Indonesia. Total of 93 milk samples used in this study, there were 49 (52.7%) subclinical and 3 (3.2%) clinical mastitis samples. From 52 subclinical and clinical mastitis samples could be isolated 20 (38.5%) staphylococci. Phenotypic identification were performed based on the Gram staining, Mannitol Salt Agar (MSA), catalase, Voges-Proskauer (VP) and coagulase tests. Molecular identification were determined using the Polymerase Chain Reaction (PCR) to detect several genes such as 23S rRNA, nuc and coa genes and confirmed with DNA sequencing. Based on phenotypic and genotypic identification, could be identified 10 Coagulase Positive Staphylococci (CPS) indicated Staphylococcus aureus and 10 Coagulase Negative Staphylococci (CNS) isolates, including Staphylococcus pasteuri (3 isolates), Staphylococcus xylosus (5 isolates) and Staphylococcus haemolyticus (2 isolates). From 3 clinical mastitis of PE goats in our study could be isolated three different staphylococci i.e., S. aureus, S. pasteuri and S. xylosus. This finding indicated that both CPS and CNS have important role as causative pathogen of mastitis in PE goats.]]>Al-Mathkhury, H.J.F., M.T. Flaih and Z.K.A. Al-Ghrairy,2008Staphylococcus xylosus isolated from patients with urinary tract infections.]]>11123130Altschul, S.F., W. Gish, W. Miller, E.W. Myers and D.J. Lipman,1990215403410Banerjee, S.N., T.G. Emori, D.H. Culver, R.P. Gaynes and W.R. Jarvis et al.,19919186S89SBarriere, C., R. Bruckner and R. Talon,2001Staphylococcus xylosus.]]>6740964104Bleu, U., K. Sacher, S. Corti and U. Braun,2006159677679Bochniarz, M., W. Wawron and M. Szczubial,201316487492Brakstad, O.G., K. Aasbakk and J.A. Maeland,1992Staphylococcus aureus by polymerase chain reaction amplification of the nuc gene.]]>3016541660Chesneau, O., A. Morvan, F. Grimont, H. Labischinski and N. El Solh,1993Staphylococcus pasteuri sp. nov., Isolated from human, animal and food specimens.]]>43237244Contreras, A., D. Sierra, A. Sanchez, J.C. Corrales, J.C. Marco, M.J. Paape and C. Gonzalo,200768145153Cremonesi, P., M. Luzzana, M. Brasca, S. Morandi and R. Lodi et al.,2005Staphylococcus aureus enterotoxigenic strains isolated from milk and dairy products.]]>19299305Czerw, M., J. Molenda, K. Kosek-Paszkowska, J. Bystron and M. Kotowicz,200763104106El-Jakee, J.K., N.E. Aref, A. Gomaa, M.D. El-Hariri, H.M. Galal, S.A. Omar and A. Samir,201317478Greig, J.D., E.C.D. Todd, C.A. Bartleson and B.S. Michaels,20077017521761Goh, S.H., S.K. Byrne, J.L. Zhang and A.W. Chow,1992Staphylococcus aureus on the basis of coagulase gene polymorphisms.]]>3016421645Harrigan, W.F.,19983rd Edn.,Pages: 532Pages: 532Hookey, J.V., J.F. Richardson and B.D. Cookson,1998Staphylococcus aureus based on PCR restriction fragment length polymorphism and DNA sequence analysis of the coagulase gene.]]>3610831089Leitner, G., S. Sela, O. Hammer-Muntz, D. Zivotofsky, L. Weisblit, M. Chaffer and S. Zamir,2009Staphylococcus haemolyticus.]]>7615Malinowski, E., H. Lassa, A. Kłossowska, H. Markiewicz, M. Kaczmarowski and S. Smulski,200650349352Meugnier, H., M. Bes, C. Vernozy-Rozand, C. Mazuy, Y. Brun, J. Freney and J. Fleurette,1996Staphylococcus xylosus and Staphylococcus equorum strains isolated from goat milk and cheese.]]>31325331Muehlherr, J.E., C. Zweifel, S. Corti, J.E. Blanco and R. Stephan,20038638493856Purnomo, A., Hartatik, Khusnan, S.I.O. Salasia and Soegiyono,2006Staphylococcus aureus of milk of Etawa crossbred Goat.]]>22142147Salasia, S.I.O., Z. Khusnan, C. Lammler and M. Zschock,2004Staphylococcus aureus isolated from bovine subclinical mastitis in central Java in Indonesia and Hesse in Germany.]]>5103109Salasia, S.I.O., S. Tato, N. Sugiyono, D. Ariyanti and F. Prabawati,2011Staphylococcus aureus isolated from bovines, humans and food in Indonesia.]]>12353361Savini, V., C. Catavitello, A. Bianco, A. Balbinot and D. D'Antonio,2009Staphylococcus pasteuri: From mammals and lampreys, to man.]]>4123129Scherrer, D., S. Corti, J.E. Muehlherr, C. Zweifel and R. Stephan,2004Staphylococcus aureus isolates from raw bulk-tank milk samples of goats and sheep.]]>101101107Straub, J.A., C. Hertel and W.P. Hammes,1999Staphylococcus aureus in meat starter cultures and dairy products.]]>6211501156Taponen, S., H. Simojoki, M. Haveri, H.D. Larsen and S. Pyorala,2006115199207Taponen, S., J. Koort, J. Bjorkroth, H. Saloniemi and S. Pyorala,20079033013307Pyorala, S. and S. Taponen,200913438Waller, K.P., A. Aspan, A. Nyman, Y. Persson and U.G. Andersson,2011152112116