Research Journal of Microbiology1816-4935xxxx-xxxxScience International10.3923/jm.2015.502.512Bacillus cereus Isolate]]>GargSatyendra Kumar TripathiM. LalN. 1120151011In the present study, different approaches are being compared for the decolorization of reactive orange 4 monoazo dye by Bacillus cereus isolate under varied cultural and nutritional conditions. By employing conventional one-factor-at-a-time approach, the bacterial strain exhibited decolorization activity over a wide range of pH (7.0-9.0), temperature (30-38°C), dye concentration (50-200 mg L1 MSM) and inoculum size (1.0-6.0%, v/v), with peak activity (68.2% color removal) at pH 8.0, 35°C, 50 mg dye L1 MSM and 4.0% (v/v) inoculum in the presence of 1.0% (w/v) glucose as carbon/energy source and 0.2% (w/v) ammonium nitrate within 72 h incubation. Under response surface methodology (RSM using Box-Behnken design) approach, the dye decolorization enhanced to 100% at optimized 40 mg reactive orange L1 MSM, glucose 1.0% (w/v) and ammonium nitrate 0.2% (w/v) during 72 h of incubation. The dye decolorization time was advanced by 12 h in bioreactor trial and 100% color removal was achieved within only 60 h incubation. In future experimentation, we envisage to test the potential of our isolate for the decolorization of other variety of azo dyes, mixture of dyes as well as the real textile effluent.]]>Alalewi, A. and C. Jiang,20123889901Ali, N., A. Hameed and S. Ahmed,201041907915Asad, S., M.A. Amoozegar, A.A. Pourbabaee, M.N. Sarbolouki and S.M.M. Dastgheib,20079820822088Bayoumi, R.A., S.M. Husseiny, A.S. Bahobil, S.S. Louboudy and T.A. El-Sakawey,20101125Hao, O.J., H. Kim and P.C. Chiang,200030449505Khehra, M.S., H.S. Saini, D.K. Sharma, B.S. Chadha and S.S. Chimni,20067017Padmavathy, S., S. Sandhya, K. Swaminathan, Y.V. Subrahmanyam, T. Chakrabarti and S.N. Kaul,200317147151Pandey, A., P. Singh and L. Iyengar,2007597384Patel, Y., C. Mehta and A. Gupte,201275187193Ponraj, M., K. Gokila and V. Zambare,20112168177Saratale, R.G., S.S. Gandhi, M.V. Purankar, M.B. Kurade, S.P. Govindwar, S.E. Oh and G.D. Saratale,2013Lysinibacillus sp. RGS.]]>115658667Sarioglu, M. and T. Bisgin,200775544549Tripathi, M., S. Vikram, R.K. Jain and S.K. Garg,2011516169Tony, B.D., D. Goyal and S. Khanna,200963462469Wang, H., J.Q. Su, X.W. Zheng, Y. Tian, X.J. Xiong and T.L. Zheng,2009Citrobacter sp. CK3.]]>63395399Zimmermann, T., H.G. Kulla and T. Leisinger,1982Pseudomonas KF46.]]>129197203Liao, C.S., C.H. Hung and S.L. Chao,2013Bacillus cereus strain HJ-1.]]>9021092114Lourenco, N.D., J.M. Novais and H.M. Pinheiro,200042321328Meitiniarti, V.I., E.S. Soetarto, K.H. Timotius and E. Sugiharto,2007Enterococcus faecalis ID6017 and Chryseobacterium indologenes ID6016.]]>15154Chen, K.C., W.T. Huang, J.Y. Wu and J.Y. Houng,1999Proteus mirabilis.]]>23686690Dayaram, P. and D. Dasgupta,2008Polyporus rubidus.]]>29831836