Research Journal of Microbiology1816-4935xxxx-xxxxScience International10.3923/jm.2013.70.80M. Al-OtaibiMutlag S. Al-ZorekyNajeeb A. El-DermerdashHassan 2201382Generally, fresh milk is taken as raw product with or without proper preservation.
Fresh milk is subjected to bacterial contamination irrespective of its source
and can cause health problems if consumed as fresh product. Camel is one of
the favorite animals in the Middle East Countries. Studies on isolating active
LAB from camels milk in Saudi Arabia are scarce. The main objective of
this study was to isolate potential probiotic Lactic Acid Bacteria (LAB). Phenotypic
characteristics were determined by phenotypical and biochemical tests, carbohydrate
assimilation by using API 50CH strips and API 50 CHL medium and probiotic activity
of isolates was determined by tolerating bile salts. The Cell Free Supernatants
(CFS) were sensitive to proteolytic enzymes indicating the proteinaceous nature
(i.e., bacteriocins) of inhibitors. There was no significant difference (p>0.05)
in weight gain among control and treated mice. The counts (log10
CFU mL-1) of test isolates were not significantly (p>0.05) affected
by bile slats in MRS broth. In conclusion, LAB isolates proved useful and can
exhibit probiotic activities in albino mice which are able to prevent the adhesion
of Staphylococcus aureus and to inhibit the E. coli in Gastrointestinal
(GI) tract.]]>Adnan, A.F.M. and I.K. Tan,20079813801385Alegria, A., S. Delgado, C. Roces, B. Lopez and B. Mayo,2010Lactococcus lactis strains isolated from traditional, starter-free cheeses made of raw milk.]]>1436166Holt, J.G.,19778th Edn.,Cheikhyoussef, A., N. Pogori, W. Chen and H. Zhang,2008125215222Chen, H. and D.G. Hoover,2003282100De Vuyst, L. and F. Leroy,200713194199Ercolini, D., I. Ferrocino, A. La Storia, G. Mauriello, S. Gigli, P. Masi and F. Villani,201027137143Giraffa, G.,20112011Jans, C., J. Bugnard, P.M.K. Njage, C. Lacroix and L. Meile,201247371379Jiang, J., B. Shi, D.Q. Zhu, Q.X. Cai and Y.R. Chen et al.,2012Lactobacillus sakei LSJ618 isolated from traditional Chinese fermented radish.]]>23338344Liu, S.N., Y. Han and Z.J. Zhou,201144643651Malheiros, P.S., V. Sant'Anna, M. Utpott and A. Brandelli,2012234247Medici, M., C.G. Vinderola, R. Weill and G. Perdigon.2005Escherichia coli infection in mice.]]>72243249Millette, M., G. Cornut, C. Dupont, F. Shareck, D. Archambault and M. Lacroix,2008419972003Morandi, S. and M. Brasca,2012Streptococcus thermophilus strains isolated from north Italian traditional cheeses.]]>23203209Nikolic, M., A. Terzic-Vidojevic, B. Jovcic, J. Begovic, N. Golic and L. Topisirovic,2008122162170Ravishankar, S., L. Zhu, C.W. Olsen, T.H. McHugh and M. Friedman,200974M440M445Ricci, G., F. Borgo, C. Ferrario and M. Fortina,2011Lactobacillus strains.]]>116Ripamonti, B., A. Agazzi, C. Bersani, P. de Dea and C. Pecorini et al.,20111797105SAS.,200121st Edn.,Settanni, L. and A. Corsetti,2008121123138Shu, Q., S.J. Zhou, K.J. Rutherfurd, M.J. Birtles, J. Prasad, P.K. Gopal and H.S. Gill,1999Lactobacillus acidophilus HN017, Lactobacillus rhamnosus HN001 and Bifidobacterium lactis HN019) have no adverse effects on the health of mice.]]>9831836Vesterlund, S., M. Karp, S. Salminen and A.C. Ouwehand,2006Staphylococcus aureus adheres to human intestinal mucus but can be displaced by certain lactic acid bacteria.]]>15218191826Yateem, A., M.T. Balba, T. Al-Surrayai, B. Al-Mutairi and R. Al-Daher,20083194199