Research Journal of Microbiology1816-4935xxxx-xxxxScience International10.3923/jm.2012.41.49Saccharomyces cerevisiae]]>WahabN.A. NoorZ.M. AbdullahM.F.F. 1201271An E. coli yeast shuttle vector for the anchoring of heterologous protein to the yeast hosts cell wall was constructed. The vector PYDSM01 includes a DNA sequence constructed from the signal sequence from the yeast sucrose isomerase gene, a multiple cloning site and a DNA fragment encoding the carboxyl-terminal of the yeast cell wall protein 2 (CWP2). This construct was then inserted into the HindIII site on pGAD424, replacing the GAL4 fusion tag and the original MCS sequence. DNA sequencing confirmed the correct insertion of both signal and anchor proteins in the vector. To test for proper expression and functional anchoring to the cell wall, the coding sequence for a bacterial alpha-amylase enzyme was cloned into the vector and transformed into a yeast host. A total of 22 yeast transformant were recovered which were able to degrade starch, indicating successful expression and function of the bacterial alpha-amylase gene. Enzyme assay of the washed cell pellet and supernatant fractions indicate that the both activity and anchoring efficiency are variable.]]>Abe, H., Y. Shimma and Y. Jigami,2003In vitro oligosaccharide synthesis using intact yeast cells that display glycosyltransferases at the cell surface through cell wall-anchored protein Pir.]]>138795Steyn, A.J. and I.S. Pretorius,1991Saccharomyces diastaticus glucoamylase-encoding gene and a Bacillus amylo-liquefaciens amylase-encoding gene in Saccharomyces cerevisiae.]]>1008593Boder, E.T. and K.D. Wittrup,199715553557Breinig, F. and M.J. Schmitt,2002Saccharomyces cerevisiae.]]>58637644Breinig, F., B. Diehl, S. Rau, C. Zimmer, H. Schwab and M.J. Schmitt,2006Saccharomyces cerevisiae and its enhancement by constitutive activation of the cellular unfolded protein response.]]>7271407147Fujita, Y., S. Takahashi, M. Ueda, A. Tanaka and H. Okada et al.,20026851365141Kondo, A. and M. Ueda,2004642840Kuroda, K., S. Shibasaki, M. Ueda and A. Tanaka,200157697701Kuroda, K. and M. Ueda,200363182186Matsumoto, T., M. Ito, H. Fukuda and A. Kondo,200464481485Murai, T., M. Ueda, M. Yamamura, H. Atomi and Y. Shibasaki et al.,19976313621366Murai, T., M. Ueda, H. Atomi, Y. Shibasaki and N. Kamasawa et al.,1997Saccharomyces cerevisiae.]]>48499503Murai, T., M. Ueda, T. Kawaguchi, M. Arai and M. Tanaka,1998Aspergillus aculeatus.]]>6448574861Murai, T., M. Ueda, Y. Shibasaki, N. Kamasawa, M. Osumi, T. Imanaka and A. Tanaka,1999516570Nakamura, Y., S. Shibasaki, M. Ueda, A. Tanaka, H. Fukuda and A. Kondo,200157500505Pepper, L.R., Y.K. Cho, E.T. Boder and E.V. Shusta,200811127134Routledge, E.J. and J.P. Sumpter,199727232803288Schreuder, M.P., S. Brekelmans, H. Van Den Ende and F.M. Klis,1993Saccharomyces cerevisiae.]]>9399409Shibasaki, S., M. Ueda, K. Ye, K. Shimizu, N. Kamasawa, M. Osumi and A. Tanaka,200157528533Shimma, Y., F. Saito, F. Oosawa and Y. Jigami,2006Saccharomyces cerevisiae.]]>7270037012Shiraga, S., M. Kawakami, M. Ishiguro and M. Ueda,20057143354338Van der Vaart, J.M., R. te Biesebeke, J.W. Chapman, H.Y. Toschka, F.M. Klis and C.T. Verrips,1997Saccharomyces cerevisiae as anchors for cell surface expression of heterologous proteins.]]>63615620Wahab, N.A., Z.M. Noor and M.F.F. Abdullah,20112011pp: 113118Gietz, D., A. St Jean, R.A. Woods and R.H. Schiestl,19922014251425