Research Journal of Microbiology1816-4935xxxx-xxxxScience International10.3923/jm.2012.131.138Shewanella oshoroensis sp. nov.: A Mesophilic Eicosapentaenoic Acid and Hentriacontanonaene-producing Bacterium]]>SugiharaShinji Iskandar Bin Haji Mohd TahaAhmad MotoigiTaro UenoAkio ShimizuSatoru SatoMamiko KanekoTakako WatanabeKentaro YumotoIsao OkuyamaHidetoshi 2201272A mesophilic marine bacterial strain (strain osh08T) that produced eicosapentaenoic acid and hentriacontanonaene, was identified by the molecular and ordinary microbiological methods. This strain had the high sequence similarity of its 16S rRNA gene to those of Shewanella basaltis JCM 14937T (EU143361; 99.8%) and Shewanella hafniensis NBRC 100975T (AB205566; 98.4%). But, strain osh08T was phenotypically different from S. basaltis J83T and S. hafniensis NBRC 100975T in that it was able to ferment glucose, produce β-glucosidase and β-galactosidase and utilize D-mannose and unable to utilize malate. However, all three strains used iron(III) and manganese(IV) as terminal electron acceptors under anaerobic conditions. The DNA-DNA relatedness between strain osh08T and S. basaltis J83T and that between strain osh08T and S. hafniensis NBRC 100975T were 4.7% and 11.8%, respectively. The G+C content of strain osh08T was 43.4 mol%. Strain osh08T had, in addition to a polar flagellum, many pilus-like structures around the cell that were not observed in the cells of S. basaltis J83T. Thus strain osh08T was identified as a new species of Shewanella. The name Shewanella oshoroensis sp. nov. was proposed and strain osh08T (=NBRC 107685T) is the type strain.]]>Barrow, G.I. and R.K.A. Feltham,19933rd Edn.,Pages: 331Pages: 331Brettar, I., R. Christen and M.G. Hofle,2002Shewanella denitrificans sp. nov. a vigorously denitrifying bacterium isolated from the oxic-anoxic interface of the Gotland Deep in the central Baltic Sea.]]>5222112217Bozal, N., M.J. Montes, E. Tudela, F. Jimenez and J. Guinea,2002Shewanella frigidimarina and Shewanella livingstonensis sp. nov. Isolated from Antarctic coastal areas.]]>52195205Chang, H.W., S.W. Roh, K.H. Kim, Y.D. Nam, C.O. Jeon, H.M. Oh and J.W. Bae,2008Vibrio areninigrae sp. nov., a marine bacterium isolated from black sand.]]>5819031906Ezaki T., Y. Hashimoto and E. Yabuuchi,198939224229Felsenstein, J.,198539783791Haschemeyer, R.H. and R.J. Meysers,19721972pp: 114-117pp: 114-117Marmur, J.,19613208218Myers C.R. and K.H. Nealson,198824013191321Nichols, D.S., P.D. Nichols and T.A. McMeekin,199531:9 polyene hydrocarbon from Antarctic bacteria.]]>125281285Orikasa, Y., T. Nishida, A. Hase, K. Watanabe, N. Morita and H. Okuyama,2006Moritella marina strain MP-1.]]>58044234429Satomi, M., H. Oikawa and Y. Yano,2003Shewanella marinintestina sp. nov., Shewanella schlegeliana sp. nov. and Shewanella sairae sp. nov., novel eicosapentaenoic-acid-producing marine bacteria isolated from sea-animal intestines.]]>53491499Satomi, M., B.F. Vogel, L. Gram and K. Venkateswaran,2006Shewanella hafniensis sp. nov. and Shewanella morhuae sp. nov., isolated from marine fish of the Baltic Sea.]]>56243249Sugihara, S., R. Hori, H. Nakanowatari, Y. Orikasa and Y. Takada et al.,201045167177Sukovich, D.J., J.L. Seffernick, J.E. Richman, J.A. Gralnick and L.P. Wackett,20107638503862Tamaoka, J. and K. Komagata,198425125128Tamaoka, J., M. Yanagibayashi, C. Kato and K. Horikoshi,19981998pp: 319Venkateswaran, K., D.P. Moser, M.E. Dollhopf, D.P. Lies and D.A. Saffarini et al.,1999Shewanella and description of Shewanella oneidensis sp. nov.]]>49705724Yoon, J.H., K.H. Kang, T.K. Oh and Y.H. Park,2004Shewanella gaetbuli sp. nov., a slight halophile isolated from a tidal flat in Korea.]]>54487491ZoBell, C.E.,1946