Research Journal of Microbiology1816-4935xxxx-xxxxScience International10.3923/jm.2011.735.749Pseudomonas sp.]]>SahuG.K. SindhuS.S. 102011610Siderophore production by rhizosphere bacteria contributes to fungal growth inhibition resulting into suppression of plant diseases. Therefore, siderophore-producing Pseudomonas isolates were studied for root rot disease control and plant growth stimulation of green gram. Fifty-eight Pseudomonas strains were obtained from the rhizosphere soil of chickpea and green gram using dilution plating technique. These strains were tested for siderophore production on MM9 medium and succinate medium plates containing chromo-azurol S by spot test method. Siderophore-producing strains formed the halo zone around the colony growth and diameter of halo zone varied with different Pseudomonas strains. Siderophore production was found more on MM9 medium as compared to succinate medium. Bacterial colony growth (G) and halo zone (H) size ratios varied from 1.44 to 10.24 in different Pseudomonas strains on MM9 medium. Siderophore-producing Pseudomonas strains inhibited the growth of phytopathogenic fungi i.e., Fusarium oxysporum, Rhizoctonia solani and Pythium aphanidermatum. Large zones of fungal growth inhibition were observed on PDA medium in comparison to nutrient agar medium plates. Seed inoculation of Pseudomonas cultures in green gram (Vigna radiata L.) caused reduction in root rot disease (R. solani induced) symptoms under pot house conditions and disease control varied from 33.4 to 100% with different Pseudomonas cultures. Coinoculation of different Pseudomonas strains with Bradyrhizobium strain SMR15 in green gram also enhanced the nodule number, nodule fresh weight and plant dry weight at 30 and 60 days of plant growth. Coinoculation of Pseudomonas strain CP56 with Bradyrhizobium strain and R. solani showed maximum 275.8% increase in plant dry weight at 60 days in comparison to control uninoculated plants and completely suppressed the root rot disease under pot house conditions. Thus, inoculation with siderophore-producing Pseudomonas sp. could be utilized for disease control and plant growth promotion of legumes.]]>Abeysinghe, S.,2009Bacillus subtilis CA32 and Trichoderma harzianum RU01 on biological control of Rhizoctonia solani on Solanum melongena and Capsicum annuum.]]>8916Ahemad, M. and M.S. Khan,2011Pseudomonas putida under insecticide-stress.]]>15464Ahmad, F., I. Ahmad and M.S. Khan,2008168173181Baig, M.M.V., M.I.A. Baig and S.M. Muley,2002226063Becker, J.O. and R.J. Cook,1988Pythium species and production of increased-growth response of wheat by fluorescent pseudomonads.]]>78778782Benizri, E., E. Baudoin and A. Guckert,200111557574Bolton, H.Jr., L.F. Elliott, R.F. Turco and A.C. Kennedy,1990Rhizobium leguminosarum and a deleterious root colonizing Pseudomonas sp. and effects on plant growth.]]>123121124Boruah, H.P.D. and B.S. Kumar,2002Pseudomonas strain.]]>47137143Brisbane, P.G. and A.D. Rovira,1988Gaeumannomyces graminis var. tritici by fluorescent pseudomonads.]]>37104111Buyer, J.S. and L.J. Sikora,1990129101107Buysens, S., K. Heungens, J. Poppe and M. Hofte, 1996Pythium-induced damping-off of tomato by Pseudomonas aeruginosa 7 NSK2. ]]>62865871Crowley, D.E. and S.M. Kraemer,20072007pp: 73-109pp: 73-109Dar, G.H., M.A. Beig, F.A. Ahanger, N.A. Ganai and M.A. Ahangar,2011Rhizoctonia solani and Fusarium oxysporum in blue pine (Pinus wallichiana) through use of fungal antagonists.]]>56274De Bellis, P. and G.L. Ercolani,20016719451948Derylo, M. and A. Skorupska,1993Pseudomonas.]]>154211217Kumar, B.S.,1996Bacillus sp. SR2 from peanut rhizosphere.]]>34794798Duhan, J.S., S.S. Dudeja and A.L. Khurana,19982 fixation and iron uptake in pigeonpea-Rhizobium symbiosis.]]>43421426Duijff, B.J., P.A.H.M. Bakker and M. Schippers,1994Fusarium wilt of carnation by Pseudomonas putida WCS358 at different levels of disease incidence and iron availability.]]>4270288Ehteshamul-Haque, S. and A. Ghaffar,1993138157163Gill, P.R. and G.J. Warren,198817163170Goel, A.K., S.S. Sindhu and K.R. Dadarwal,2000Pseudomonas sp.: Inhibition of fungal growth and stimulation of growth of Cicer arietinum.]]>43563569Gupta, A., A.K. Saxena, M. Gopal and K.V.B.R. Tilak,199857726736Hynes, R.K., G.C. Leung, D.L. Hirkala and L.M. Nelson,200854248258King, E.O., M.K. Ward and D.E. Raney,195444301307Kloepper, J.W., J. Leong, M. Teintze and M.N. Schroth,1980286885886Ksiezniak, A. and J. Kobus,19931027790Lemanceau, P., A. Robin, S. Mazurier and G. Vansuyt,20072007pp: 165-192pp: 165-192Leong, J.,198624187209Mahmoud, A.L.E. and M.A. Abd-allah,2001Bradyrhizobium-mungbean symbiosis.]]>3157162Mayer, J.M. and M.A. Abdallah,1978Pseudomonas fluorescens: Biosynthesis, purification and physicochemical properties.]]>107319328Meyer, J.M. and M.A. Abdallah,1980Pseudomonas stutzeri.]]>118125129Miller, H.J., G. Henken and J.A. van Veen,198935656660Neiland, J.B.,198150715731Neilands, J.B.,198236285309Osman, M.E.H., M.M. El-Sheekh, M.A. Metwally, A.E.A. Ismail and M.M. Ismail,2011Rhizoctonia solani.]]>2101114Peter, N.K. and D.P.S. Verma,1990348Raaijmakers, J.M., M. Leeman, M.M.P. van Oorschot, I.V. Sluis, B. Schippers and P.A.H.M. Bakker,1995Pseudomonas spp.]]>8510751081Rachid, D. and B. Ahmed,2005Pseudomonas fluorescens.]]>4697702Saber, W.I.A., K.M. Abd El-Hai and K.M. Ghoneem,2009Trichoderma and Rhizobium on both biocontrol of chocolate spot disease and induction of nodulation, physiological activities and productivity of Vicia faba.]]>4286300Sambrook, J., E.F. Fritsh and T. Maniatis,1989Scherwinski, K., R. Grosch and G. Berg,2008Rhizoctonia solani and negligible, short-term effects on nontarget microorganisms.]]>64106116Schwyn, B. and J.B. Neilands,1987Rhizobiaceae.]]>195114Schwyn, B. and J.B. Neilands,19871604756Sharma, A. and B.N. Johri,2003Pseudomonas strain GRP3A in mung bean (Vigna radiata L. Wilzeck).]]>1587781Sharma, A. and B.N. Johri,2003Pseudomonas strains GRP3A and PRS9 in maize (Zea mays L.) under iron limiting conditions.]]>158243248Siddiqui, I.A., S. Ehetshamul-Haque and S.S. Shaukat,2001149337346Simeon, L.A., W.L. Lindsay and R. Baker,19877710571063Sindhu, S.S., S.K. Gupta and K.R. Dadarwal,1999Pseudomonas spp. on pathogenic fungi and enhancement of growth of green gram (Vigna radiata).]]>296268Sindhu, S.S., Y.S. Rakshiya and G. Sahu,200931021Sindhu, S.S., S. Dua, M.K. Verma and A. Khandelwal,20102010pp: 195-235pp: 195-235Sindhu, S.S., S. Suneja, A.K. Goel, N. Parmar and K.R. Dadarwal,2002Pseudomonas sp. on coinoculation with Mesorhizobium sp. Cicer strain under sterile and wilt sick soil conditions.]]>195764Sloger, C.,19692 fixation in nodulated soybean.]]>4416661668Somasegaran, P. and H.J. Hoben,1994Rhizobium Technology.]]>pp: 450pp: 450Stockwell, V.O. and J.P. Stack,2007Pseudomonas spp. for integrated biological control.]]>97244249Tahat, M.M., K. Sijam and R. Othman,20109198207Validov, S., F. Kamilova, S. Qi, D. Stephan, J.J. Wang, N. Makarova and B. Lugtenberg,2007Fusarium oxysporum f.sp. radicis-lycopersici in stonewool substrate.]]>102461471Van Rossum, D., A. Muyotcha, H.W. van Verseveld, A.H. Stouthamer and F.C. Boogred,1994Bradyrhizobium sp. strains nodulating groundnut.]]>163177187Verma, A. and S. Chincholkar,2007pp: 248pp: 248Vikram, A. and H. Hamzehzarghani,2008Vigna radiata L. Wilczek).]]>36272Weller, D.M.,2007Pseudomonas biocontrol agents of soilborne pathogens: Looking back over 30 years.]]>97250256Weyens, N., D. van der Lelie, S. Taghavi, L. Newman and J. Vangronsveld,200927591598Yeole, R.D., B.P. Dave and H.C. Dube,200139464464Woyessa, D. and F. Assefa,2011Eragrostis tef Zucc. Trotter) under greenhouse condition.]]>6343355Alexander, D.B. and D.A. Zuberer,1991Rhizosphere bacteria.]]>123945Chebotar, V.K., C.A. Asis and S. Akao,200134427432