Research Journal of Microbiology1816-4935xxxx-xxxxScience International10.3923/jm.2011.669.677E. coli and E. coli O157: H7 Isolates from a Typical Tropical Abattoir on Wood, Steel and Glass Surfaces]]>AdetunjiVictoria Olusola IsolaTajudeen Opeyemi 8201168Adhesion of bacteria to meat processing surfaces may act as potential sources of transmission of pathogens in meat industry. Several studies have shown that adhesion of bacteria to surfaces partly depends upon the nature of the inert surfaces and partly upon the bacterial surface properties. The aim of this study was to compare the adhesion of E. coli and E. coli O157: H7 on wood, glass and steel surfaces. Twenty abattoir isolates of E. coli (8) and E. coli O157:H7 (12) from meat tables in Ibadan municipal abattoir, Nigeria were assayed for bacterial adhesion. Bacterial adhesion was assessed on wood, glass and steel surfaces at ambient temperature for 24 and 72 h. Attachment to surfaces was quantified using a crystal violet binding assay. In this study, E. coli isolates adhered on all the three surfaces studied, viz. wood, glass and steel. The isolates varied in their ability to adhere to the surfaces. The adhesion by isolates at 24 and 72 h were significantly different (p<0.05). The mean absorbance values (nm) at 24 h showed that E. coli O157:H7 (SEH1) adhere more bacterial cells on wood (0.51±0.02), steel (0.15±0.00) and glass (0.12±0.01) surfaces than E. coli (SE) which was wood (0.38±0.02), steel (0.13±0.01) and glass (0.07±0.01). At 24 and 72 h, a significant difference (p<0.05) was observed between the isolates for wood and glass. However, at 24 h, a significant difference (p<0.01) ensued between E. coli O157:H7 and E. coli for steel. E. coli and E. coli O157:H7 adhere more bacterial cells on wood than on steel and glass. Bacterial adhesion is influenced by strains with high virulence factors and an extension of incubating period. Proper surface selection can reduce the ability of bacteria adhesion on meat contact surfaces.]]>Abrishami, S.H., B.D. Tall, T.J. Brunrsema, P.S. Epstein and D.B. Shah,199414153172Adetunji, V.O.,2010Listeria monocytogenes and Escherichia coli 0157: H7 strains isolated from wara a West African soft cheese.]]>4249254Alves, M.M., M.A. Pereira, J.M. Novais, F.F. Polanco and M. Mota,199971209217Azad, A.K., H. Fauzia and S.M. Badiar Rahman,2003Escherichia coli in tap water and raw milk samples.]]>6770773Barners, L.M., M.F. Lo, M.R. Adams and A.H. Chamberlain,19996545434548Barrow, G.I. and R.K.M. Feltham,19933rd Edn.,Pages: 331Pages: 331Batool, R. and H. Shahida,2005Enterobacter and Serratia isolated from biofilms on plant growth and soil aggregation.]]>4347353Characklis, W.G.,19901990pp: 17-54pp: 17-54Carpentier, B. and O. Cerf, 199375499511Davey, E.M. and A.G. O'Toole,200064847867Dewanti, R. and A.C.L. Wong,1995Escherichia coli O157:H7.]]>26147164Djordjevic, D., M. Wiedmann and L.A. McLandsborough, 2002Listeria monocytogenes biofilm formation.]]>6829502958Donlan, R.M.,20028881890Dunsmore, D.G., A. Twomey, W.G. Whittlestone and H.W. Morgan,198144220240El-Abed, S., A. Houari, H. Latrache, A. Remmal and S.I. Koraichi,2011In vitro activity of four common essential oil components against biofilm-producing Pseudomonas aeruginosa.]]>6394401Fletcher, M. and G.I. Loeb,1979376772Fonseca, A.P., P.L. Granja, J.A. Nogueira, R. Oliveira and M.A. Barbosa,2001Staphylococcus epidermidis RP62A adhesion to chemically modified cellulose derivatives.]]>12543548Frank, J.F., 200143319370Gengeorgis, C.,19951995pp: 29-47pp: 29-47Gibson, H., J.H. Taylor, K.E. Hall and J.T. Holah,1999874148Gulsun, S., N. Oguzoglu, A. Inan and N. Ceran,2005Escherichia coli isolated from recurrent urinary tract infections.]]>2617551758Hood, S.K. and E.A. Zottola,19956918Hyde, F.W., M. Alberg and K. Smith,199719142147Kanwal, R., A. Toqeer, S.S. Tahir and R. Naseem,2004Bacillus cereus and E. coli towards lead, copper, iron, manganese and arsenic.]]>769Lauzon, H.L.,19981998Leclercq-Perlat, M.N. and M. Lalande,199423501517Karunasagar, I., S.K. Otta and I. Karunasagar,1996Vibrio harveyi on surfaces.]]>140241245Kumar, C.G. and S.K. Anand,199842927Kusumaningrum, H.D., G. Ribodi, W.C. Hazeleger and R.R. Beurner,200385227236Manijeh, M., J. Mohammad and K.K. Roha,20083181186Manijeh, M., J. Mohammad and K.K. Roha,2008Salmonella enteritidis on food contact surfaces.]]>8502505Mao, Y., M.P. Doyle and J. Chen,2001Escherichia coli O157:H7.]]>18338113815Moltz, A.G. and S.E. Martin,2005Listeria monocytogenes under various growth conditions.]]>689297Midelet, G. and B. Carpentier,200497262270Movassagh, M.H. and A.R. Karami,2010Escherichia coli: O111 on food contact glass surfaces.]]>4222224Pawar, D., M. Rossman and J. Chen,2005Escherichia coli to attach to abiotic surfaces.]]>99418425Prigent-Combaret, C., G. Prensier, T.T. Le Thi, O. Vidal, P. Lejeune and C. Dorel,2000Escherichia coli strains: Role of flagella, curli and colanic acid.]]>2450464Puyate, Y.T. and A. Rim-Rukeh,2008815601565Riley, L.W., R.S. Remis, S.D. Helgerson, H.B. McGee and J.G. Wells et al.,1983Escherichia coli serotype.]]>308681685Ryu, J.H., H. Kim, J.F. Frank and L.R. Beuchat,2004Escherichia coli O157:H7 as affected by curli production.]]>39359362Ryu, J.H., H. Kim and L.R. Beuchat,2004Escherichia coli O157:H7 on stainless steel as influenced by exopolysaccharide production, nutrient availability and temperature.]]>6721232131Rendon, M.A., Z. Saldana, A.L. Erdem, V. Monteiro-Neto and A. Vazquez et al.,2007E. coli use a common pilus adherence factor for epithelial cell colonization.]]>1041063710642Rodriguez, A.S. and L.A. Mclandsborough,2007Listeria monocytogenes from stainless steel and high density polyethylene to biological or American cheese.]]>70602606Salamitou, S., M.J. Kirkpatrick, H.M. Ly, G. Leblon, E. Odic and M.S. DuBow,20098228234Sinde, E. and J. Carballo,2000Salmonella spp. and Listeria monocytogenes to stainless steel, rubber and polytetrafluorethylene: The influence of free energy and the effect of commercial sanitizers.]]>17439447Snyder, P.,19971997Soomro, A.H., M.A. Arain, M. Khaskheli and B. Bhutto,2002Escherichia coli from raw milk and milk products in relation to public health sold under market conditions at Tandojam, Pakistan.]]>1151152Stepanovic, S., I. Cirkovic, L. Ranin and M. Svabic-Vlahovic,2004Salmonella spp. and Listeria monocytogenes on plastic surface.]]>28326432Stopforth, J.D., J. Samelis, J.N. Sofos, P.A. Kendall and G.C. Smith,2003Escherichia coli O157:H7 in biofilms.]]>6622582266Teixeira, P. and R. Oliveira,19991312871294Uhlich, G.A., J.E. Keen, and R.O. Elder,2001Escherichia coli O157:H7.]]>6723672370Wells, J.G., B.R. Davis, I.K. Wachsmuth, L.W. Riley, R.S. Remis, R. Sokolow and G.K. Morris,1983Escherichia coli serotype.]]>18512520Whittam, T.S., I.K. Wachsmuth and R.A. Wilson,1988Escherichia coli O157:H7 associated with hemorrhagic colitis and hemolytic uremic syndrome.]]>15711241133