Research Journal of Microbiology1816-4935xxxx-xxxxScience International10.3923/jm.2011.552.559AminiS. ZulkiflyA.H. Wen-YongWong HuyopF. 6201166Most halogenated compounds are major environmental pollutants. Pollution from industrial chemicals and herbicide/pesticide from agricultural activities cause problem to the society. The main objectives were to isolate and characterize the bacterium that can grow in halogenated substrate as the sole source of carbon. A common microbiological and molecular techniques were adapted for further characterization. In current study, a soil microorganism, identified as Aminobacter sp. SA1 by partial biochemical and 16S rRNA sequencing method was isolated with 2,2DCP (2,2-dichloropropionic acid) as the sole carbon and energy source. This bacterium has the ability to degrade 2,2DCP (2,2-dichloropropionic acid) and D,L2CP (D,L-2-chloropropionic acid) at 20 mM with cells doubling time of 7 h in 2,2DCP medium. The bacterium was able to grow at low substrate concentration of 1 mM 2,2DCP (20 times lower than normal growth concentration) with cells doubling time of approximately 7 h suggesting the dehalogenase enzyme(s) that present in the bacterium has high affinity towards the substrate. However, at 0.1 mM 2,2DCP (200 times lower than normal concentration) no halide ions were detected in the growth medium suggesting no growth was observed. The investigation highlighted degradation at low substrate concentration suggested that the isolated bacterium can live in low level of halogenated compounds. This observation may or may not involve new kinds of dehalogenases. The presence of more than one dehalogenases in a bacterial system is far from clear.]]>Allison, N., A.J. Skinner and R.A. Cooper,198312912831293Ashton, F.M. and A.S. Crafts,1973Pages: 504Pages: 504Bergman, J.G. and J. Sanik,195729241243Duddleston, K., D. Arp and P. Bottomley,20023 oxidizing bacteria.]]>59535539Fetzner, S. and F. Lingens,199458641685Fulton, C.K. and R.A. Cooper,2005Mycobacterium sp. CF1.]]>7378381Haggblom, M.M. and I.D. Bossert,20042004pp: 3-29pp: 3-29Hamid, A.A.A., S. Hamdan, S.H.Z. Ariffin and F. Huyop,201010190199Hammond, M.E.R. and A. Cooper,2002Spartina anglica eradication and inter-tidal recovery in Northern Ireland estuaries. Turning the tide: Eradication of invasive species.]]>2002pp: 124132Hardman, D.J.,199111140Hareland, W.A., R.L. Crawford, P.J. Chapman and S. Dagley,1975Pseudomonas acidovorans.]]>121272285Holt, J.G., N.R. Krieg, P.H.A. Sneath, J.T. Staley and S.T. Willams,19949th Edn.,Pages: 787Pages: 787Huyop, F. and M. Nemati,2010Rhizobium sp. RC1.]]>428362847Huyop, F., T.Y. Yusn, M. Ismail, R.AB. Wahab and R.A. Cooper,2004Rhizobium sp.]]>121520Ismail, S.N., A.M. Taha, N.H. Jing, R.A. Wahab, A.A. Hamid, R.V. Pakingking Jr. and F. Huyop,2008Pseudomonas sp. strain R1 bacterium isolated from Malaysian paddy (rice) field.]]>7481486Jing, N.H. and F. Huyop,2007Rhodococcus sp.]]>15147151Jing, N.H. and F. Huyop,2008Methylobacterium sp. HJ1.]]>8233235Jing, N.H., R.A. Wahab, A.M. Taha, N.A.A. Rashid and F. Huyop,2008Rhodococcus sp. HJ1.]]>3482488Leisinger, T. and R. Bader,199347116121McDonald, I.R., P. Kampfer, E. Topp, K.L. Warner and M.J. Cox et al.,2005Aminobacter ciceronei sp. nov. and Aminobacter lissarensis sp. nov., isolated from various terrestrial environments.]]>5518271832McDonald, R., K.L. Warner, C. McAnulla, C.A. Woodall, R.S. Oremland and J.C. Murrell,20024193203Muller, E.E.L., F. Bringel and S. Vuilleumier,20112011Olaniran, A.O., G.O. Babalola and A.I. Okoh,2001454550Olaniran, A.O., D. Pillay and B. Pillay,2004552733Saitou, N. and M. Nei,19874406425Schwarze, R., A. Brokamp and F.R.J. Schmidt,199734103109Sorensen, S.R., M.S. Holtze, A. Simonsen and J. Aamand,2007Aminobacter spp. isolated from dichlobenil treated soils.]]>73399406Tamura, K., M. Nei and S. Kumar,20041011103011035Tamura, K., J. Dudley, M. Nei and S. Kumar,20072415961599Thasif, S., S. Hamdan and F. Huyop,20098264269Warner, K.L., M.J. Larkin, D.B. Harper, J.C. Murrell and I.R. McDonald,2005Aminobacter lissarensis CC495.]]>2514551