Research Journal of Microbiology1816-4935xxxx-xxxxScience International10.3923/jm.2011.510.523AbulreeshH.H. OrganjiS.R. 6201166The aim of this survey was to investigate the incidence of Staphylococcus aureus and coagulase-negative staphylococci and their antibiogram in food and the environment in Makkah, western Saudi Arabia. Staphylococci were detected using selective plating, employing Staphylococcus medium no. 110 and mannitol salt agar. Confirmation of isolates was carried out using Gram staining, production of catalase, coagulase and type of blood heamolysis. Disk diffusion method was used to test susceptibility patterns of staphylococci against seven antibiotics. The results showed that staphylococci were present in almost all types of samples. Staph. aureus were prevalent in raw milk, cheese, mucous membranes and to a degree, in biofilm. Multidrug-resistance was noted in both coagulase-positive and coagulase-negative isolates from all types of samples. 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