Research Journal of Microbiology1816-4935xxxx-xxxxScience International10.3923/jm.2011.328.342Streptomyces roseodistaticus TA15 and Streptomyces lavendofoliae TA17]]>Al-HumianyAbd Ul-Rahman Abd Allaa4201164Isolation of antimicrobial agents from actinomycetes isolated from EL-Taif area, Kingdom of Saudi Arabia, two streptomyces isolates showed remarkable antimicrobial activity against tested organisms. The two isolates were previously identified according to the recommended keys as Streptomyces roseodistaticus TA15 and Streptomyces lavendofoliae TA17. The active metabolites of St. roseodistaticus TA15 and St. lavedofoliae TA17 were extracted by using different organic solvents, purified using both Thin Layer Chromatography (TLC) and column chromatography. The active compounds were concentrated and investigated for their physical characteristics and elemental, spectroscopic and biochemical analysis and their Minimum Inhibition Concentration (MIC) were determined. The cytopathic effect of the two antimicrobial agents on the vero cell virus, the neutralization test in monolayer cell cultures for antiviral test on coxasackie B4, hepatitis A and herpes simplex (cox B4, HAV and HSVI) viruses was also determined. Aim of this study is to identify the antimicrobial activity of actinomycetes extracts isolated from Saudi Arabia. We suggested the name "Taificidin 1" and "Taificidin 2" for the antimicrobial agent which extracted from St. roseodistaticus TA15 and from St. lavedofoliae TA17 as two active compounds isolated for first time from EL-Taif region, KSA.]]>Abe, M., D. Inoue, K. Matsunaga, Y. Ohizumi, H. Ueda, T. Asano and Y. Sato,2002190109116Al-Humiany, A.A.,20062006Ammor, M.S., A.B. Florez, A.H. Van-Hoek, C.G.D.L. Reyes-Gavilan, H.J. Aarts, A. Margolles and B. Mayo,200814615Augustine, S.K., S.P. Bhavsar and B.P. Kapanis,2005Streptomyces albidoflafus PU 23.]]>30201211Berdy, J.,1980152835Berdy, J.,19801st Edn., Vol. 1,Berdy, J.,19801st Edn., Vol. 2,Bilinov, N.O. and A.S. Khokhlov,1970pp: 34-57pp: 34-57Edwin, H.L.,19694th Edn.,El-Naggar, M.Y.,2007Streptomyces violaceusniger strain HAL64.]]>45262267El-Tayeb, O.M., A.A. Salama, M.M.M. Hussein and H.F. El-Sedawy,2004Amycolatopsis mediterranei I. The role of colony morphology and nitrogen sources in productivity.]]>3266272Boudjella, H., K. Bouti, A. Zitouni, F. Mathieu, A. Lebrihi and N. Sabaou,2005161288298Honda, Y., M. Ueki, G. Okada, R. Onose, R. Usami, K. Horikoshi and H. Osada, 2001Curvularia sp. RK 97-F 166.]]>541016Kavanagh, F.,1972Vol. 2,Pages: 631Pages: 631Lazzarini, A., L. Cavaletti, G. Toppo and F. Marinelli,200078399405Tsukamoto, M., S. Nakajima, K. Murooka, M. Naito and H. Suzuki et al.,2000Streptomyces.]]>53687693Ronald, M.A.,19962nd Edn.,Shearer, M.C.,199728198Ueno, R., N. Urano, M. Suzuki and S. Kimuna,20022 from glucose at 40°C.]]>177244250Umezawa, H.,1967Weinstein, M.J. and G.H. Wagman,1978Zitouni, A., H. Boudjella, F. Mathieu, N. Sabaou and A. Lebrihi,2004Saccharothrix sp. SA 103. I. Taxonomy, fermentation, isolation and biologicalactivities.]]>57367372Zitouni, A., L. Lamari, H. Boudjella, B. Badji and N. Sabaou et al.,2004Saccharothrix algeriensis sp. nov., isolated from Saharan soil.]]>5413771381Abbanat, D., W. Maiese and M. Greenstein,199952117126Gupte, M.D. and P.R. Kalkarni,2000Streptomyces chattanoogensis MTCC 3423 using full factorial design.]]>352226Jones, L., B. Bartholomew, Z. Latif, S.D. Sarker and R.J. Nash,2000Cassia laevigata.]]>71580583Projan, S.J.,20036427430