Research Journal of Microbiology1816-4935xxxx-xxxxScience International10.3923/jm.2009.67.74Trichoderma longi, Aspergillus niger and Saccharomyces cerevisae Cultured on Plantain Peel]]>OmojasolaP.F. JilaniO.P. 2200942In this study, three fungi: Trichoderma longibrachiatum,
Aspergillus niger and Saccharomyces cerevisiae were cultured
on plantain peel, a cellulosic waste. The waste was dried, pre-treated
with alkali and steam, re-dried and then blended. The powdered waste was
then used as substrate in shake-flasks which contained Mineral Salts Medium
(MSM) and inoculi of the three test fungi. Fermentations were initially
carried out in flasks containing the MSM, waste substrate and the inoculum
at pH 5.0, 1% substrate concentration, 10% inoculum size and cultured
on a rotary shaker at 29 ± 1 °C for 5 days to verify cellulase
production by the organisms from the waste substrates, then for 7 or 9
days while varying different fermentation parameters. Cellulase activity
and amount of glucose produced by the three test organisms from the waste
substrate was determined and compared. Glucose production was optimized
by varying the fermentation parameters: Time, pH, Substrate concentration,
Inoculum size and Temperature. The results obtained from the fermentations
showed that Trichoderma longibrachiatum produced the highest amount
of glucose among the cultures tested (1.64 mg mL-1). This was
produced from plantain peel at pH 5.0 and temperature of 45 °C on
day 7 of fermentation. The highest amount of glucose produced by Aspergillus
niger from plantain peel was 1.18 mg mL-1 at pH 4.5 and
temperature of 45 °C on day 7 of fermentation. The highest amount
of glucose produced by Saccharomyces cerevisiae was 1.00 mg mL-1
at pH 3.5 and temperature of 45 °C on day 5 of fermentation.]]>Baig, M.M.V., M.L.B. Baig, M.I.A. Baig and M. Yasmeen,20043447450Brien, D.J.O. and J.C. Jr. Craig,199644699704Brown, J.A., S.A. Collin and T.M. Wood,1987Penicillium pinophilum, mutant strain NTG III/6.]]>9176180Caritas, U.O. and C.N. Humphrey,2006Garcinia kola (bitter Kola) pulp waste on the production of CM-cellulase and β-glucosidase using Aspergillus niger.]]>5819822Chaturvedi, V., 20011st Edn.,pp: 8-21pp: 8-21Ghose, T.K.,198759257268Haapela, R., E. Parkkinen, P. Susminen and S. Unko, 1995Trichoderma reesei in shake flask cultures. ]]>43815821Jeffries, T.W.,19961996Jostein, G. and E. Johnny,19801980pp: 283-327pp: 283-327Lynd, L.R., P.J. Weimer, W.H. van Zyl and I.S. Pretorius,200266506577Mandels, N. and E.T. Reese, 195979816826Milala, M.A., A. Shugaba, A. Gidado, A.C. Ene and J.A. Wafar,2005Aspergillus niger. ]]>1325328Miller, G.L.,195931426428Narasimha, G., A. Sridevi, B. Viswanath, M.S. Chandra and R.B. Rajasekhar,2006Aspergillus niger.]]>5472476Omojasola, P.F., O.P. Jilani and S.A. Ibiyemi,200866479Srivastava, S.K., K.S. Gopalkrishnan and K.B. Ramachandran, 1987 Aspergillus wentii. ]]>659599Tang, L.G., D.N.S. Hon, S.H. Pan, Y.Q. Zhu, Z. Wang and Z.Z. Wang,199659483488Umeh, I.B.,19981998pp: 84-120pp: 84-120Wang, X.J., J.G. Bai and Y.X. Lian,200673533540Osagie, A.U. and O.U. Eka,1998Pages: 279Pages: 279