Research Journal of Microbiology1816-4935xxxx-xxxxScience International10.3923/jm.2009.278.285Klebsiella pneumoniae Producing CTX-M-15 Genes from Neonatal Intensive Care Unit in Saudi Arabia]]> Al-AgamyM.H.M. ShiblA.M. TawfikA.F. ElbannaiA.R. 7200947Reports on outbreak of extended-spectrum β-lactamases (ESβLs) by Enterobacteraiceae and especially Klebsiella pneumoniae, are few in Saudi Arabia. This study was therefore devoted to describe the outbreak which occurred by ESβL-producing K. pneumoniae. Sixteen K. pneumoniae isolates were isolated from 16 neonatal patients hospitalized from September 2007 to December 2007 in the neonatal intensive care unit during the outbreak in Al-Qatif Hospital, Eastern Province, Saudi Arabia. These isolates were sent to microbiological laboratories, College of Pharmacy, King Saud University, for investigation. Klebsiella pneumoniae strains were found to produce antibiotic resistance and produce extended spectrum beta-lactamase. Genotypic characterization of extended spectrum beta-lactamase producing K. pneumoniae showed that all isolates carried TEM-1, SHV-1 and CTX-M-15 genes. Matting out assay revealed that all third generation cephalosporins were located on transferable plasmid. An outbreak which occurred in neonatal intensive care unit was due to CTX-M-15-producing K. pneumoniae isolates either single or in multiple clones. This is the first report of blaCTX-M-15 gene in Saudi Arabia from K. pneumoniae and the first outbreak in Saudi hospitals due to CTX-M-15 producing K. pneumoniae.]]>Abbassi, M.S., C. Torres, W. Achour, L. Vinue and Y. Saenz et al.,200632308314Al-Agamy, M.H.M., M.S. El-Din Ashour and I. Wiegand,200627545548Al-Obeid, S., S. Bremont, L. Jabri, N. Massoudi and Q. Haddad, 200820709713Baraniak, A., J. Fiett, W. Hryniewicz, P. Nordmann and M. Gniadkowski, 200250393396Bonnet, R., C. Dutour, J.L.M. Sampaio, C. Chanal and D. Sirot et al., 20014522692275Canton, R. and T.M. Coque, 20069466475Carrer, A., L. Lassel, N. Fortineau, M. Mansouri, N. Anguel, C. Richard and P. Nordmann,2009154754Chanawong, A., F.M. M'Zali, J. Heritage, J.H. Xiong and P.M. Hawkey,2002Enterobactericeae in the peoples rebublic of China.]]>46630637CLSI.,20067th Edn.,Pages: 49Pages: 49Conceicao, T., A. Brizio, A. Duarte, L.M. Lito, J.M., Cristino and M.J. Salgado, 200549477478Coque, T.M., A. Oliver, J.C. Perez-Diaz, F. Baquero and R. Canton, 2002Klebsiella pneumoniae clones in a single hospital (Madrid, 1989 to 2000). ]]>46500510De Oliveira, G.D., Y. Doi, D. Szabo, J.M. Adams-Haduch and T.M. Vaz et al.,2008Klebsiella pneumoniae producing extended-spectrum beta-lactamase CTX-M-2 and novel variant CTX-M-59 in a neonatal intensive care unit in Brazil.]]>5217901793Ensor, V.M., W. Jamal, V.O. Rotimi, J.T. Evans and P.M. Hawkey, 2009Escherichia coli and Klebsiella pneumoniae from hospital and community patients in Kuwait. ]]>33487489Hobson, R.P., F.M. MacKenzie and I.M. Gould, 199633249249Karim, A., L. Poirel, S. Nagarajan and P. Nordmann, 2001201237241Kliebe, C., B.A. Nies, S.F. Meyer, R.M. Tolxdorff-Neutzling and B. Wiedeman, 198528302307Livermore, D.M. and N. Woodford, 2006Enterobacteriaceae, Pseudomonas and Acinetobacter.]]>14413420Livermore, D.M., R. Canton, M. Gniadkowski, P. Nordmann and G.M. Rossolini et al.,200759165174Nordmann, P. and L. Poirel,20028321331Nuesch-Inderbinen, M.T., F.H. Kayser and H. Hachler,199741943949Nukaga, M., K. Mayama, A.M. Hujer, R.A. Bonomo and J.R. Knox, 2003328289301Poirel, L., C. Heritier, V. Tolun and P. Nordmann,2004Klebsiella pneumoniae.]]>481522Quale, J.M., D. Landman, P.A. Bradford, M. Visalli and J. Ravishankar et al., 2002Klebsiella pneumoniae infection. ]]>35834841Randrianirina, F., S. Vedy, D. Rakotovao, C.E. Ramarokoto and H. Ratsitohaina et al., 2009Klebsiella pneumoniae producing SHV-2 and CTX-M-15 extended-spectrum beta-lactamases. ]]>722329Rebuck, J.A., K.M. Olsen, P.D. Fey, A.N., Langnas and M.E. Rupp, 2000Klebsiella pneumoniae in a paediatric intensive care unit transplant population. ]]>3113681372Sanger, F., S. Nicklen and A.R. Coulson,19777454635467Soge, O.O., A.M. Queenan, K.K. Ojo, B.A. Adeniyi and M.C. Roberts, 2006Klebsiella pneumoniae.]]>572430Vatopoulos, A.C., A. Philippon and L.S. Tzouvelekis, 1990Klebsiella pneumoniae and Escherichia coli in Greece. ]]>2627552759Villegas, M.V., A. Correa, F. Perez, M.C. Miranda and T. Zuluaga et al., 2004Klebsiella pneumoniae and Escherichia coli isolates from Colombian hospitals. ]]>49217222Yigit, H., A.M. Queenan, G.J. Anderson, A. Domenech-Sanchez and J.W. Biddle et al.,2001Klebsiella pneumoniae.]]>4511511161