Research Journal of Microbiology1816-4935xxxx-xxxxScience International10.3923/jm.2008.499.507ThampayakIntira CheepthamNaowarat Pathom-AreeWasu LeelapornpisidPimporn LumyongSaisamorn 7200837Two hundred and twenty-nine soil actinomycete strains
were initially screened for extracellular biosurfactant activity by a
drop-collapse method in Kim`s medium containing sesame oil as a sole source
of carbon. Three isolates, namely S71, S72 and S177, were capable of biosurfactant
production. Phenotypic and genotypic analysis strongly suggested that
they were members of the genus Streptomyces. The isolates S71 and
S177 were closely related to S. griseoflavus sharing 99% 16S rRNA
gene similarities, whereas S72 was closely related to S. fradiae
sharing only 98% 16S rRNA gene similarities suggesting that this may represent
a novel species. The cell-free culture broth of the three isolates had
emulsification activity and decreased surface tension. According to emulsification
activity (E24) and surface tension values observed in the three isolates,
Streptomyces sp. S72 was selected for biosurfactant production
in larger scale. The cell-free culture broth of the isolate S72 was further
extracted with chloroform:methanol (2:1) and two fractions were found
positive in producing biosurfactants. To determine structure and molecular
weight of the two positive fractions, the Nuclear Magnetic Resonance (NMR)
spectroscopy and Mass Spectrometry (MS) will be carried out.]]>Augustine, S.K., S.P. Bhavsar and B.P. Kapadnis,2005Streptomyces albidoflavus PU 23.]]>30201211Barabas, G., G. Vargha, I.M. Szabo, A. Penyige and S. Damjanovich et al.,2001 Streptomyces strains. ]]>79269276Becker, B., M.P. Lechevalier, R.E. Gordon and H.A. Lechevalier,1964Nocardia and Streptomyces by paper chromatography of whole-cell hydrolysates.]]>12421423Benson, D.A., I. Karsch-Mizrachi, D.J. Lipman, J. Ostell and D.L. Wheeler, 200432D2326Bodour, A.A. and R.M. Miller-Maier,199832273280Boone, C.J. and L. Pine,196816279284Cooper, D.G. and B.G. Goldenberg,1987Bacillus species.]]>53224229Desai, J.D. and I.M. Banat,1997614764Duvnlak, Z., D.G. Cooper and N. Kosaric, 1982Arthrobacter paraffineus ATCC 19558.]]>24165175Fitch, W.M. and E. Margoliash, 2001155279284Franzetti, A., G. Bestetti, P. Caredda, P. La Colla and E. Tamburini,2008Gordonia strains.]]>63238248Hayakawa, M. and H. Nonomura,198765501509Hickey, R.T. and H.D. Tresner,1952Streptomyces species.]]>64891892Imada, C., 2005875963Jukes, T.H. and C.R. Cantor,19691969pp: 21-132pp: 21-132Kelly, K.L., 195861427427Kieser, T., M.J. Bibb, M.J. Buttner, K.F. Chater and D.A. Hopwood, 20002000pp: 162-170pp: 162-170Kim, S.H., E.J. Lim, S.O. Lee, J.D. Lee and T.H. Lee, 2000Nocardia sp. L-417.]]>31249253Kluge, A.G. and F.G. Farris, 196918132Lang, S. and D. Wullbrandt, 1999512232Lin, S.C., 199666109120Maidak, B.L., J.L. Cole, T.G. Lilbum, C.T. Parker Jr. and P.R. Saxman et al.,200129173174Maier, R.M., 200352102121Makkar, R.S. and S.S. Cameotra,1997Bacillus strains at thermophilic conditions.]]>74887889Oskay, A.M., T. Usame and A. Cem,20043441446Pizzul, L., M.P. Castillo and J. Stenstrom, 200622745752Pridham, T.G., C.W. Hesseltine and R.G. Benedict,195865279Richter, M., J.M. Willey, R. Suβmuth, G. Jung and H.P. Fiedler,1998Streptomyces tendae Tu 901/8c.]]>163165171Robert, M., M.E. Mercade, M.P. Bosch, J.L. Parra, M.J. Espuny, M.A. Manresa and J. Guinea, 1989Pseudomonas aeruginosa 44T.]]>11871874Saitou, N. and M. Nei, 19874406425Van de Peer, Y. and R. de Wachter, 199410569570Wheeler, D.L., D.M. Church, A.E. Lash, D.D. Leipe and T.L. Madden et al.,2001291116Williams, S.T., M. Goodfellow, G. Alderson, E.M.H. Wellington, P.H.A. Sneath and M.J. Sackin,1983Streptomyces and related genera.]]>12917431813Youssef, N.H., K.E. Duncan, D.P. Nagle, K.N. Savage, R.M. Knapp and M.J. McInerney,200456339347