Research Journal of Microbiology1816-4935xxxx-xxxxScience International10.3923/jm.2008.482.488Rhodococcus sp.HJ1]]>Hong JingNg Ab. WahabRoswanira Mohd TahaAishah Abdul RashidNoor Aini HuyopFahrul 6200836 The main aim of the present study is to further characterize
a new dehalogenase enzyme found in the crude extracts from Rhodococcus
sp. The ability of the enzyme to catalyze the dehalogenation
of various halogen-substituted organic acids was investigated and the
highest activity was found with 3-chloropropionic acid as a sole carbon
source in the growth medium. The enzyme followed Michaelis-Menten kinetics
and the Km for 3-chloropropionic acid was 0.2 mM. Maximum activity was
found at pH 7.6 at 30°C. The enzyme activity in the cell-free extract
was unaffected by diaminoethane tetraacetic acid (EDTA), dithiothreitol
(DTT) or by Mn and Zn ions but was reduced by HgCl2 (70%) and
Pb(NO3)2 (80%). The enzyme removed the chlorine
atom present on a number of 3- and 4-carbon alkanoic acids if the halogen
was on the β-position.]]>Bergman, J.G. and J. Sanik,195729241243Hardman, D.J. and J.H. Slater,1981123117128Hareland, W.A., R.L. Crawford, P.J. Chapman and S. Dagley,1975Pseudomonas acidovorans.]]>121272285Holt, J.G., N.R. Kreig, P.H.A. Sneath, J.T. Staley and S.T. Williams,19949th Edn.,Pages: 787Pages: 787Iwasaki, I., S. Utsumi, K. Hagino and T. Ozawa,195629860864Janssen, D.B., J.E. Oppentocht and G.J. Poelarends,200112254258Jing, N.H., A.M. Taha, R.V. Pakingking, Jr., R.A.B. Wahab and F. Huyop,2008Methylobacterium sp. HJ1 induced by the herbicide 2,2-dichloropropionate (Dalapon).]]>23236Kawasaki, H., H. Tone and K. Tonomura,1981Moraxella sp. strain.]]>453542Kerr, L.M. and J.R. Marchesi,200664848855Marchesi, J.R. and A.J. Weightman,200354854Motosugi, K., N. Esaki and K. Soda,1982Pseudomonas sp.]]>150522527Olaniran, A.O., G.O. Babalola and A.I. Okoh,2001454550Olaniran, A.O., D. Pillay and B. Pillay,2004552733Park, C., T. Kurihara, T. Yoshimura, K. Soda and N. Esaki,200323329336Schwarze, R., A. Brokamp and F.R.J. Schmidt,199734103109Song, J.S., D.H. Lee, K. Lee and C.K. Kim,200341277283Torz, M. and V. Beschkov,2005Xanthobacter autotrophicus GJ10 strain in batch and continuous culture.]]>16423433Van Pee, Karl-Heinz and S. Unversucht,200352299312Weightman, A.J., A.L. Weightman and J.H. Slater,1982Pseudomonas putida PP3: Evidence of two different dehalogenation mechanisms.]]>12817551762