Research Journal of Microbiology1816-4935xxxx-xxxxScience International10.3923/jm.2008.359.367Escherichia coli Isolated Using Molecular Approaches]]>ALHajNagi AhmedMarianaN.S. RahaA.R. IshakZ. 5200835Escherichia coli strains are among the major bacterial causes of diarrheal illness. There are now seven classes of diarrheagenic E. coli (DEC), namely enteropathogenic E. coli (EPEC), enterohaemorrhagic E. coli (EHEC), enteroinvasive E. coli (EIEC), enterotoxigenic E. coli (ETEC), enteroaggregative E. coli (EAEC), diarrhea-associated hemolytic E. coli (DHEC) and Cytolethal Distending Toxin (CDT)-producing E. coli. Due to the need for costly and labor-intensive diagnostic procedures, identification of DEC is difficult at standard laboratories. Therefore, Polymerase Chain Reaction (PCR) or dot blot has been used for genetic detection of DEC of 25 E. coli isolates from different sources. Amplification of eae (277 bp), bfp (266 bp), stx1 (154 bp), EAST (94 bp), stx2 (698 bp) and elt (450 bp)genes of a single product in separate reactions was produced. PCR showed ability to amplify and detected genes of the most common important categories of diarrheagenic E. coli isolates of different sources, it is possible implementation of this technique to diagnosis water, food-borne outbreaks related to E. coli. Dots blot and sequence analysis used to confirm the results of PCR.]]>Afset, J.E., K. Bergh and L. Bevanger, 2003Escherichia coli (EPEC) in Norwegian children with diarrhoea.]]>5210151019Aranda, K.R.S., U. Fagundes-Neto and I.C.A. Scaletsky, 2004Escherichia coli and Shigella sp.]]>4258495853Cerqueira-Campos, M.L., P.I. Peterkin and A.N. Sharpe, 1986Salmonella strains.]]>52124127Chakraborty, S., J.S. Deokule, P. Garg, S.K. Bhattacharya and R.K. Nandy et al.,2001Escherichia coli in an outbreak of cholera caused by Vibrio cholerae O1 and O139 in Ahmedabad, India.]]>3932413246Clarke, S.C., 2001Escherichia coli-an emerging problem?]]>419398Stoeckel, D.M. and V.J. Harwood,20077324052415Hart, C.A., R.M. Batt and J.R. Saunders, 1993Escherichia coli. ]]>13121131Johnson, J.R. and T.A. Russo, 2002Escherichia coli: The other bad E. coli.]]>139155162Kaper, J.B., 199627130133Kaper, J.B., J.P. Nataro and H.L.T. Mobley, 2004Escherichia coli.]]>2123140Levine, M.M., 1987Escherichia coli that cause diarrhea: Enterotoxigenic, enteropathogenic, enteroinvasive, enterohemorrhagic and enteroadherent.]]>155377379Nataro, J.P. and J.B. Kaper,1998Escherichia coli.]]>11142201Nguyen, R.N., L.S. Taylor, M. Tauschek and R. Robins-Browne, 2006Escherichia coli infection and prolonged diarrhoea in children.]]>12597603Nougayrede, J.P., P.J. Fernandes and M.S. Donnenberg, 2003Escherichia coli to host cells.]]>5359372Olasz, F., P.Z. Fekete, G. Blum-Oehler, Z. Boldogkoi and B. Nagy, 2005+ enterotoxigenic Escherichia coli strain from post weaning diarrhoea of swine and of its conjugative virulence plasmid pTC.]]>244281289Olivier, C., S. Bonacorsi and E. Bingen, 2004Escherichia coli strains causing neonatal meningitis.]]>4217701772Paton, A.W., P. Srimanote, U.M. Talbot, H. Wang and J.C. Paton, 2004Escherichia coli.]]>2003546Peterkin, P.I., E.S. Idziak and A.N. Sharpe, 1991Listeria monocytogenes by direct colony hybridization on hydrophobic grid-membrane filters by using a chromogen-labeled DNA probe.]]>57586591Russo, T.A. and J.R. Johnson,2003Escherichia coli: Focus on an increasingly important endemic problem.]]>5449456Samadpour, M., J. Liston, J.E. Ongerth and P.I. Tarr, 1990Escherichia coli in food and calf fecal samples.]]>5612121215Samadpour, M., J.E. Ongerth, J. Liston, N. Tran and D. Nguyen et al.,1994Escherichia coli in retail fresh seafood, beef, lamb, pork and poultry from grocery stores in Seattle, Washington.]]>6010381040Schmidt, H. and M. Hensel, 2004171456Stacy-Phipps, S.J., J. Mecca and J.B. Weiss, 1995Escherichia coli DNA during the course of infection.]]>3310541059Thorpe, C.M., 2004Escherichia coli infection.]]>3812981303Todd, E.C., R.A. Szabo, J.M. MacKenzie, A. Martin and K. Rahn et al
1999Escherichia coli.]]>6547754780