Research Journal of Microbiology1816-4935xxxx-xxxxScience International10.3923/jm.2008.269.275Rhizobium Species F1]]>BoboyeB. AlaoA. 4200834Rhizobium species F1 was studied for its ability to grow in the presence of trehalose (Trehalose-Minimal Medium (TMM)) and absence of it (Nutrient Broth (NB)) as sole carbon and energy sources and form Trehalose-Catabolic-Enzyme (TCE). The organism was mutagenized with hydroxylamine. The resultant mutants and the parental strain were grown with and without trehalose. The supernatants of the grown culture alone and lysed cells in supernatants were assayed for the activity of TCE. Rhizobium species F1 and the mutants grew in TMM and NB. Many of the mutants grew better (OD670 = 0.36-1.0 in TMM and OD670 = 0.005-0.99 in NB) than the wild-type (OD670 = 0.51 in TMM and OD670 = 0.25 NB). All the strains constitutively and inducibly expressed the trehalose-catabolic enzyme with a range of 0.242-1.42 mg mL-1 glucose mg-1 mL-1 protein for the mutants and 1.025 mg mL-1 glucose mg-1 mL-1 protein for the parental type. In the absence of trehalose in the growth medium, the mutants synthesized higher amount of the TCE with the highest value of 1.091 mg mL-1 glucose mg-1 mL-1 protein and then the wild-type which exhibited enzyme activity of 0.321 mg mL-1 glucose mg-1 mL-1 protein. The enzyme was extracellularly and intracellularly expressed in the TMM and NB. Activity of the total trehalose-degrading enzyme was higher than that of the extracellular. Three classes of the mutants were identified. Low, normal and super-trehalose-catabolic-enzyme producers showed enzyme activity in the ranges of 0 to 30, 31 to 60 and above 60 mg mL-1 glucose mg-1 mL-1 protein, respectively.]]>Bhumiratana, A., R.L. Anderson and R.N. Costilow,1974Bacillus popillae.]]>119484493Boboye, B. and O. Shonukan,1993Xanthomonas campestris.]]>9240242Boboye, B.,2004Rhizobium species NGR234.]]>97256261Boos, W., U. Ehmann, H. Forkl, W. Klein, M. Rimmele and P. Postma,1990Escherichia coli.]]>17234503461Brock, T.D., D.W. Smith and M.T. Madigan,19844th Edn.,Broughton, W.J. and M.J. Dilworth,197112510751080Dardanelli, M.S., P.S. Gonzalez, M.A. Bueno and N.E. Ghittoni,200040149456Elbein, A.D.,197430227256Finar, I.L.,19865th Edn.,pp: 942pp: 942Fisbein, L., W.G. Flamin and H.L. Falk,1970pp: 364pp: 364Gornall, A.J., C.S. Bardawill and M.M. David,1949177751751Harley, J.L. and S.E. Smith,1983Jensen, J.B., T.V. Bhuvaneswari, O.Y. Ampomah, R. Darrah and N.K. Peters,2005Sinorhizobium meliloti-alfalfa interactions.]]>18694702Kadowaki, M.K., M. de Lourdes, T.M. Polizeli, H.F. Terenzi and J.A. Jorge,1995Scytalidium thermophilum.]]>1291199205Karim, S., H. Aronsson, H. Fricson, M. Pirthonen and B. Leyman et al.,200764371386Lopez, M.F. and J.G. Torrey,1985Frankia ArI3.]]>143209215Louis, P., H.P. Truper and E.A. Galinski,1994Escherichia coli during drying and storage in the presence of compatible solutes.]]>41684688Madigan, M., P. Martino and J. Parker,20019th Edn.,pp: 204-208pp: 204-208Martinez-Esparza, M., A. Aguinaga, P. Gonzalez-parraga, P. Garcia-penarrubia, T. Jouault and J.C. Arguelles,2007Candida albicans.]]>13384394Moran, F. and M.P. Starr,1969Erwinia.]]>11291295Muller, J., R.A. Aeschbacher, A. Wingler, T. Boller and A. Wiemken,2001Arabidopsis.]]>12510861093Parkinson, J.S.,1976CheA, CheB and CheC genes of Escherichia coli and their role in chemotaxis.]]>126758770Pedreno, Y., P. Gonzalez-parraga, M. Martinez-esparza, R. Sentandreu, E. Valentin and J.C. Arguelles,2007Candida albicans ATC1 gene encoding a cell-linked acid trehalase decreases hypha formation and infectivity without affecting resistance to oxidative stress,]]>15313721381Postma, P.W., H.G. Keizer and P. Koolwijk,1986Salmonella typhimurium.]]>16811071111Sampedro, J.G., Y.G. Ruiz-granados, H. Najera, A. Tellez-valencia and S. Uribe,2007+-ATPase from Kluyveromyces lactis.]]>4656165622Streeter, J.G.,1985Rhizobium bacteria and bacteroids.]]>1647884Streeter, J.G. and M.L. Gomez,2006Bradyrhizobium cultured bacteria and in bacteroids from soybean nodules.]]>7242504255Veluthambi, V., S. Mahaderan and E. Zimmermann,1981Cuscuta reflexa: Correlation with low trehalase activity.]]>6813691374Willis, L.B. and G.C. Walker,1999Sinorhizobium meliloti operon encodes an -glucosidase and a periplasmic-binding-protein-dependent transport system for -glucosides.]]>18141764184Wingler, A., T. Fritzius, A. Wiemken, T. Boller and R.A. Aeschbacher,2000124105114Wolf, A., R. Krämer and S. Morbach,2003Cornebacteriuim glutamicum ATCC 13032 and their significance in response to osmotic stress.]]>4911191134