Research Journal of Microbiology1816-4935xxxx-xxxxScience International10.3923/jm.2008.181.186ManijehMahdavi MohammadJalali RohaKasra Kermanshahi3200833The biofilm is consist of microbial cell clusters with a network of internal channels or voids in the Extracellular Polymeric Substances (EPS) and glycoprotein matrix. Biofilms due to special structure and EPS are more resistant to control measures. Biofilms can remain on various surfaces which may assist the survival of pathogenic and spoilage bacteria in the food processing environment, a contributing factor in foodborne disease outbreaks. In this research bacterial strains were isolated by swabbing method from surfaces of meat processing factory environments. More than 60 different species of bacteria isolated from various segment of meat processing plant and the hydrophobicity of isolates measured by Microbial Adhesion Test to Hydrocarbon (MATH) method for screening of isolates. The quantity of biofilm of isolates with high hydrophobicity was determined using microtiter plate assay method and ELISA reader machine. Results indicated Bacillus megaterium and Staphylococcus epidermidis with 45 and 33% of hydrophobicity have the highest potential in biofilm formation. Pathogenic S. aureus with 30% of hydrophobicity classified under moderately adherent. B. subtilis with 22% of hydrophobicity considered as weakly adherent. Micrococcus varians and M. roseus with 1 and 5% of hydrophobicity were non adherent. The result from this study highlighted the problems of spread of bacteria. In the development of cleaning and sanitization protocol in meat processing environments, an awareness of these biofilm forming bacteria is essential for the meat processing.]]>Bower, C.K., J. McGuire and M.A. Daeschel, 19967152157Bryers, D.J., 2000Carpentier, B. and O. Cerf, 199375499511Cerca, N. and B. Pier, 2005Staphylococcus epidermidis.]]>156506514Chmielewski, R.A.N. and J.F. Frank, 200322232Cunliffe, D., C.A. Smart, C. Alexander and E.N. Vulfson, 19996549955002Den Aantrakker, D., M. Boom, H. Zwietering and M. Schothorst, 200380117130Djordjevic, D., M. Wiedmann and L.A. McLandsborough, 2002Listeria monocytogenes biofilm formation.]]>6829502958Dorthe, B., H. Mette, J. Charlotte, H. Ingrid and G. Lone, 2001Shewanella putrefaciens adhesion and biofilm formation on food processing surfaces.]]>6723192325Faille, C., F. Fontaine and T. Benezech, 2001Bacillus spores in milk product processing lines.]]>90892900Flint, S.H., P.J. Bremer and J.D. Brooks,1997118197Frank, J.F., 200143319370Gilbert, P., A.J. McBain and A.H. Rickard,200351245248Gunduz, G.T. and G. Tuncel, 200689329336Harvey, J., K.P. Keenan and A. Gilmour, 2006Listeria monocytogenes strains.]]>24380392Jeong D.K. and J.F. Frank, 1994Listeria monocytogenes at 10 C in biofilms with microorganisms isolated from meat and dairy processing environments.]]>57576586Jessen, B. and L. Lammert,200351265269Joseph, B., S.K. Otta and I. Karunasagar,2001Salmonella spp. on food contact surfaces and their sensitivity to sanitizers.]]>64367372Krieg, N.R., S.G. Holt, P.H.A. Sneath, J.T. Staley and S.T. Williams, 1998Lee Wong, A.C., 19988127652770Midelet, G. and B. Carpentier, 2002Listeria monocytogenes from various materials to beef.]]>6840154024Mittelman, M.W., 19988127602764Mozes, N. and P.G. Rouxhet,1987699112Neil, H.M., 2001Salmonella and other wee beasties in foods.]]>556167Res, M.C., T.M. Cogan and S. Tobin, 199273331336Rosenberg, M., D. Gutnick and E. Rosenberg,198092933Sharma, M. and S.K. Anand, 200219627636Sharma, M. and S.K. Anand, 200213469477Stepanovic, S., D. Vukovic, I. Dakic, B. Savic and M. Svabic-Vlahovic,2000Staphylococcal biofilm formation.]]>40175179Tompkin, R.B., 2002Listeria monocytogenes in the food-processing environment.]]>65709725Trachoo, N.,200325807815Wirtanen, G., U. Husmark and T. Mattila-Sandholm, 1996Bacillus biofilms after rinsing and cleaning procedures in closed food-processing systems.]]>59727733Wirtanen, G. and S. Salo, 20032293306Zottola, E.A. and K.C. Sasahara, 199423125148