Research Journal of Microbiology1816-4935xxxx-xxxxScience International10.3923/jm.2008.136.142Aspergilli]]>LathaR. ManonmaniH.K. RatiE.R. 3200833Aflatoxins are potent secondary metabolites produced commonly by Aspergillus flavus, A. parasiticus and A. nomius. Primers were designed specifically for o-methyl transferase (omt) and aflatoxin regulatory gene (aflR) of aflatoxin biosynthetic pathway and also to detect the genus Aspergillus specific primers (18s rRNA genes) using NS. Experimental conditions were standardized for optimum multiplex PCR. DNA extracted from mycelia of toxigenic and non-toxigenic A. flavus, A. parasiticus, other Aspergilli and from other genera of fungi were subjected to multiplex PCR using these primers. The omt and aflR primer pairs gave specific PCR amplification for aflatoxigenic A. flavus and A. parasiticus. They did not give DNA amplification for non-aflatoxigenic A. flavus, A. oryzae, A. glaucus, Fusarium, Penicillium and Rhizopus spp.]]>Abarca, M.L., M.R. Bragular, M.T. Bruguera and F.J. Cabanes,19881047579Criseo, G., A. Bagnara and G. Bisignano,2001A. flavus group.]]>33291295Doohan, F.M., G. Weston, H.W. Rezanoor, D.W. Parry and P. Nicholson,1999Tri5 byFusarium species in vitro and in planta.]]>6538503854Geisen, R.,199619388392Jungkind, D., S. Direnzo, K.G. Beavis and N.S. Silverman,19963427782783Lee, S.B., M.G. Milgroom and J.W. Taylor,1998352324Liu, B.H. and F.S. Chu,1998aflR and its product, AFLR associated with aflatoxin biosynthesis.]]>6414661469Manonmani, H.K., A. Chandrashekar and E.R. Rati,2002Aspergilli.]]>2002Marasas, W.F.O., J.D. Miller, R.T. Riley and A. Visconti,2001pp: 219pp: 219Rati, E.R., V. Prema and T. Shantha,19871 in corn, groundnut and groundnut cake.]]>249091Sambrook, J. and D.W. Russell,20012001pp: 51pp: 51Scott, P.M.,19951995Smith, J.E., C.W. Lewis, J.G. Anderson and G.L. Solomons,1994pp: 300pp: 300Somashekar, D., E.R. Rati and A. Chandrashekar,2004aflR gene for differentiation and detection of A. flavus and A. parasiticus in maize.]]>93101107Uggozzoli, L.A., J.D. Lowery, A.A. Reyes, C.I. Lin and A. Re et al.,199859214222White, T.J., T.D. Bruns, S.B. Lee and J.W. Taylor,19901990pp: 315-322pp: 315-322Young, R.K. and M.A. Cousin,2001652738Yu, J., J.W. Cary, D. Bhatnagar, T.E. Cleveland, K.P. Keller and F.S. Chu,1993Aspergillus parasiticus encoding an O-methyltransferase involved in aflatoxin biosynthesis.]]>5035643571