Research Journal of Microbiology1816-4935xxxx-xxxxScience International10.3923/jm.2007.705.716Serratia marcescens DT-1P]]>BidlanRajkumar DeepthiN. RastogiN.K. ManonmaniH.K. 102007210The cultural conditions for the production of biosurfactant by Serratia marcescens DT-1P were optimised by Response Surface Methodology (RSM). A study of complex interactions among glycerol (1-5% w/v), inoculum (1-10 OD600), pH (4-8), temperature (20-40°C) and (NH4)2SO4 (0.5-2.0%) were carried out using central composite rotatable design of experiments. The optimum conditions, which were obtained i.e., glycerol (3.4% w/v), inoculum (1.0 OD600), pH (5.8), temperature (26.6°C) and (NH4)2SO4 (0.2%) yielded 6.0 mg L-1 biomass and glycerol (5% w/v), inoculum (10.0 OD600), pH (8.0), temperature (20.0°C) and (NH4)2SO4 (0.05%), yielded 24.48 mg % of biosurfactant. The surfactant was identified as arabinolipid.]]>Arino, S., R. Marchal and J.P. Vandecasteele,1996Pseudomonas sp.]]>45162168Babu, S., A.N. Vaidya, A.S. Bal, E. Kapur, A. Jueo and P. Khanna,1996Pseudomonas aeruginosa strain BS2 from industrial wastes.]]>18263268Begley, J., B. Croft and R.J. Swannel,19964199208Brandl, H., R.A. Gross, R.W. Lenz and R.C. Fuller,1988Pseudomonas oleovorans as a source of poly(β-Hydroxyalkanoates) for potential applications as biodegradable polyesters.]]>5419771982Benat, I.M., R.S. Makkar and S.S. Cameotra,200053495508Cochran, W.G. and G.M. Cox,19572nd Edn.,DuBois, M., K.A. Gilles, J.K. Hamilton, P.A. Rebers and F. Smith,195628350356Duvnjak, Z., D.G. Cooper and N. Kosaric,1983Arthrobacter paraffineus ATCC 19558.]]>24165175Head, I.M.,1998144599608Joglekar, A.M. and A.T. May,198732857868Kairs, J.M. and M.I. Khan,199416170172Kairs, J.M. and M.I. Khan,199416258259Khuri, A.L. and J.A. Cornell,1987pp: 21-45pp: 21-45Kosaric, N.,1984pp: 659-717pp: 659-717Laycok, M.V., P.D. Hildebrand, P. Thibault, J.A. Walter and J.L.C. Wright,1991Pesudomonas flourescens.]]>39483489Layman, P.L.,1985232348Patel, R.M. and A.J. Desai,1997Pseudomonas aeruginosa GS3 from molasses.]]>259194Sawardekar, J.S., J.H. Slonekar and A. Jeanes,19673716021604Selvendran, R.R. and M.A. O`Neill,198732125153Syldatk, C., S. Lang, U. Matulovic and F.Z. Wagner,1974Pseudomonas sp.]]>40C5160Syldatk, C. and F. Wagner,19871987pp: 95-100pp: 95-100Van Dyke, M.I., H. Lee and J.T. Trevors,19919241252Yamaguchi, M., A. Sato and A. Yukayama,197617741742Georgiou, G., S. Lin and M.M. Sharma,1992106065