Research Journal of Microbiology1816-4935xxxx-xxxxScience International10.3923/jm.2007.509.515SolomonR.D. JebakumarSanthiV. Satheeja6200726The aim of present study was to compare the biological activity of plasmid purified by CsCl2 buoyant density gradient centrifugation and anion exchange HPLC elution from alkaline cell lysates. The recombinant plasmids were purified from E. coli clones by anion exchange HPLC and CsCl2 density gradient centrifugation. Purified samples were digested with restriction endonuclease and the ligated recombinant plasmids were used to transform E. coli HB101 strain. The CsCl2 purified plasmid DNA exhibited higher degree of purity than HPLC purified plasmid. However, transformation efficiency was higher for HPLC purified samples; recombinant pUC8 in VE144 varied between 2x103 to 3.5x103 for crude sample, 2.52x103 to 4.2x103 for CsCl2 density gradient purified sample and 7.5x104 to 1.15x105 transformants/μg of HPLC purified sample. The pBR322 in VE145 also showed transformation efficiency as 3.9x103 to 1.2x104 for crude sample, 5.6x104 to 6.3x105 transformants/μg of CsCl2 purified and 4.5x105 to 5.04x106 transformants/μg of HPLC purified plasmid. Due to the higher transformation efficiency and less time consumption, plasmid purification by HPLC can imply major impact on recombinant DNA technology.]]>Cohen, S.N., A.C.Y. Chang and L. Hsu,1972Escherichia coli by R factor DNA.]]>6921102115Corneau, N., C. Dube, G. LaPointe and E. Emond,2001Lactococcus lactis.]]>33711Diogo, M.M., J.A. Queiroz, G.A. Monteiro, S.A. Martins, G.N. Ferreira and D.M. Prazeres,200068576583Eon-Duval, A. and G. Burke,2004804327335Hines, R.N., K.C. O`Connor, G. Vella and W. Warren,199212430435Sambrook, J., E.F. Fritsch and T.A. Maniatis,19892nd Edn.,Pages: 397Pages: 397Moreira, K.A., M.M. Diogo, D.M.F. Prazeres, J.L. Lima Filho and A.L.F. Porto,200548113117Schluep, T. and C.L. Cooney,19982645244528Stadler, J., R. Lemmens and T. Nyhammar,20041S54S66Urthaler, J., R. Schlegl, A. Podgornik, A. Strancar, A. Jungbauer and R. Necina,2005106593106Urthaler, J., W. Buchinger and R. Necina,200552703711Weiner, M.P., T.W. Thannhauser, J.H. Laity, M.E. Benning, D.P. Lee and H.A. Scheraga,19881681858185Whisenant, E.C., B.K.A. Rasheed and Y.M. Bhatnagar,19881652025202