Research Journal of Microbiology1816-4935xxxx-xxxxScience International10.3923/jm.2007.436.444Neisseria meningitidis Serogroup A and B: Effect of Immunization with Neisseria meningitidis Serogroup A Polysaccharide and Serogroup B Outer Membrane Vesicle Conjugate as a Bivalent Meningococcus Vaccine Candidate]]>SiadatS. D.BehzadiyannejadQ. TabaraieB. AhmadiH. NorouzianD. PirayeehS. NNejatiM. ZangenehM. 5200725Bacterial meningitis caused by different groups of Neisseria meningitidis is still one of the serious health problems world wide. The Serum Bactericidal Assay (SBA) to meningococci is the most important test in immunological evaluation of meningococcal infection after vaccination. The SBA has been adapted as the gold standard for immunity against different serogroups of Neisseria meningitidis after immunization. Capsular polysaccharide of serogroup A meningococci (CPSA) was conjugated to Outer Membrane Vesicle (OMV) from a serogroup B in order to test the possibility of obtaining a bivalent serogroup A and B meningococcus immunogen. The conjugate was prepared with adipic acid hydrazide (ADH) and 1-ethyl-3-(3-dimethylamino-propyl) carbodiimide (EDAC). The conjugate and control were injected intramuscularly into groups of five rabbit with boosters on days 14, 28 and 42 after the primary immunization. The following groups were used as control: 1-CPSA plus OMV; 2-CPSA; 3-OMV and 4-normal saline. The serum collected on days 0, 14, 28, 42 and 56 were tested by complement mediated bactericidal assay according to the world health organization protocol. The results of SBA in the glycoprotein conjugate group revealed a significant increase in serum bactericidal titer against serogroup A meningococci after 56 day in comparison with the CPSA and OMV control group. Bactericidal titer against serogroup B meningococci of the conjugate showed no significant difference in comparison with the OMV containing control. The results indicate that when polysaccharide A and OMV are in a covalent conjugate form, the complex is able to induce a high level of bactericidal antibody response. Therefore, this study shows that the CPSA--OMV conjugate could be a candidate for bivalent vaccine toward serogroup A and B meningococci.]]>Ada, G. and D. Isaacs, 200397985Anderson, E.L., T. Bowers, C.M. Mink, D.J. Kendy, R.B. Belshe, H. Harakeh, L. Pais, P. Holder and G.M. Carlone, 19946233913395Anonymous,19951995Arigita, C., W. Jiskoot, J. Westdijk, C. Ingen and W.E. Hennink et al.,200422629643Balmer, P. and R. Borrow,200437787Berry, D.C., A. Van Den Kaaden, C.E. Frasch and A.B. Leussink, 2002Neisseria meningitidis serogroup a capsular polysaccharide on development of functional immune response. ]]>7037073713Bethell, D. and A.J. Pollard,200217583Cartwright, K., R. Morris, H. Rumke, A. Fox, R. Borrow, N. Begg, P. Richmond and J. Poolman,19991726122619Cartwright, K., 20011943474356Claassen, I., J. Meylis, P. Ley, C. Peeters and H. Brons et al.,1996Neisseria meningitidis hexavalent class 1 outer membrane protein containing vesicles vaccine.]]>1410011008Costantino, P., F. Norelli, A. Giannozzi, S.D. Ascenzi and A. Bartoloni et al.,19991712511263Fukasawa, L.O., M.C. Gorla, R.P. Schekman, L.R. Garcia, S.M. Carneiro, I. Raw and M.M. Tanizaki,1999 Neisseria meningitidis serogroup polysaccharide and serogroup outer membrane vesicle conjugate as a bivalent meningococcus vaccine candidate. ]]>1729512958Fukasawa, L.O., W.O. Dias, R.P.F. Schenkman, I. Raw and M.M. Tanizaki,2004Neisseria meningitidis serogroup B/C in neonatal mice.]]>41205210Gudlavalleti, S.K., A.K. Datta, Y.L. Tzeng, C. Noble, R.W. Carlson and D.S. Stephens,2004Neisseria meningitidis serogroup A capsular polysaccharide O-3 and O-4 acetyltransferase.]]>2794276542773Gupta, R.K., W. Egan, D.A. Bryla, J.B. Robbins and S. Szu, 1995Escherichia coli 0111 O-specific polysaccharide prepared by treatment with acetic acid or hydrazin bound to tetanus toxoid by two synthetic schemes.]]>6328052810Hestrin, S., 1949180249261Holst, J., B. Feiring, J.E. Fuglesang, E.A. Hoiby, H. Nokleby, I.S. Aaberge and E. Rosenqvist, 2003Neisseria meningitidis serogroup B disease. ]]>21734737Hossany, R.B., M.A. Johanson, A.A. Eniade and B.M. Pinto, 20041237433754Jennings, H.J. and C. Lugowski, 19812710111018Jensen, C., B. Kuipers, J. Van Der Bienzen, H. Cock, P. Van Der Ley and J. Tommassen,2000in vitro folded outer membrane protein PorA of Neisseria meningitidis.]]>27227233Jin, Z., C. Chu, J.B. Robbins and R. Schneerson, 20037151155120Jodar, L., K. Cartwright and I.M. Feavers, 2000Neisseria meninngitidis serogroup A and C vaccines.]]>28193197Jodar, L., I.M. Feavers, D. Salisbury and D.M. Granof, 200235914991508Lesinski, G.B. and M.A. Westerlink, 200147135149Lindberg, A.A., 1999172836Liu, T.Y., E.C. Gotschlich, E.K. Jonssen and J.R. Wysocki, 197124628492858Milagres, L.G., M.C.A. Gorla and C.T. Sacchi,19986647554761Mond, J.J., A. Lees and C.M. Snapper,199513655692Norheim, G., A. Aase, D.A. Caugant, E.A. Hoiby and E. Eritzsonn et al.,2005Neisseria meningitids.]]>2337623774Peeters, C., I. Claassen, M. Schuller, G.F.A. Kersten, Van Der Voort and J.T. Poolman, 1999Neisseria meningitidis in mice. ]]>1727022712Perez-Melgosa, M., H.D. Ochs and P.S. Linsley,2001l 3123732381Peterson, G.L., 1977et al. Which is more generally applicable.]]>83346356Rosenstin, N.E., B.A. Perkins, D.S. Stephens, T. Popovic and J.M. Hughes, 200134413781388Ruggeberg, J.U. and A.J. Pollard, 20046251266Schmidt, S., D. Zhu, V. Barniak, K. Mason, Y. Zahang, R. Arumugham and T. Metcalf, 2001Neisseria meningitidis Pora specific immune sera reduces nasopharyngeal colonization of group B meningococcus in an infant rat nasal challenge model. ]]>1948514858Schneerson, R., O. Barrera, A. Sutton and J.B. Robbins, 1980Haemophilus influenzae type b polysaccharide protein conjugates. ]]>152361376Siadat, S.D., Q. Behzadiyannejad, B. Tabaraie, H. Ahmadi and M. Nejati, 2006neisseria meningitidis serogroup b outer membrane vesicle containing pora.]]>645764Vella, P.P., J.M. Staub, J. Armstrong, K.T. Dolan and C.R. Rusk et al.,1990Haemophilus influenzae type b conjugate vaccine (meningococcal protein conjugate) (PedvaxHIB).]]>85668675World Health Organization, 19765945075