Research Journal of Microbiology1816-4935xxxx-xxxxScience International10.3923/jm.2007.260.265Toxoplasma gondii Heat Shock Protein 90]]>KazemiBahram HajjarzadehReza BandehpourMojgan HaghighiAli KhazanHooshang Seyed TabaeeSeyed Javad3200723In the present study we have isolated Toxoplasma tachyzoites from infected mice, RNA was extracted and RT-PCR reaction was conducted to obtain the Toxoplasma gondii heat shock protein 90 cDNA and gene was amplified using PCR. A 2126 bp fragment as PCR product was cloned in pGEMEX-1 expression vector and confirmed by restriction analysis.]]>Ahn, H.J., S. Kim and H.W. Nam,2003Toxoplasma gondii associated with the entry into and growth in host cells.]]>311654659Blachere, N.E., H. Udono, S. Janetzki, Z. Li, M. Heike and P.K. Srivastava,199314352356Boffy, S.A.,19841984pp: 43-50pp: 43-50Bothwell, A.L., G.D. Yancopulos and F.W. Alt,1990pp: 247-260pp: 247-260Cox, M.B. and C.A. Miller,20036415491556Echeverria, P.C., M. Matrajt, O.S. Harb, M.P. Zappia and M.A. Costas et al.,2005Toxoplasma gondii Hsp90 is a potential drug target whose expression and subcellular localization are developmentally regulated.]]>350723734Eun, H.M.,1996pp: 345-489pp: 345-489Feliciello, I. and G. Chinali,1993Escherichia coli.]]>212394401Gaastra, W. and P. Klemm,19841984pp: 269-271pp: 269-271Gaastra, W. and K. Hansen,19841984pp: 225-230pp: 225-230Hanaham, D.,1983E. coli with plasmids.]]>98503517Heike, M., B. Noll and K.H.Z.B. Meyer,199660153158Jones, J., A. Lopez and M. Wilson,20036721312138Markel, K., M. Voge and D. John,1996pp: 131-138pp: 131-138Pfeffer, U.,19981998pp: 143-151pp: 143-151Pherson, M.C. and M.J. Moller,2000Chapter 2,pp: 9-21pp: 9-21Raska, M., J. Belakova, N.K. Wudattu, L. Kafkova, K. Ruzickova, M. Sebestova, Z. Kolar and E. Weigl,2005507782Rojas, P.A., V. Martin, M. Nigro, P.C. Echeverria, E.A. Guarnera, V. Pszenny and S.O. Angel,2000Toxoplasma gondii protein belonging to the heat-shock 90 family and analysis of its antigenicity.]]>190209213Sreedhar, A.S., E. Kalmar, P. Csermely and Y.F. Shen,20045621115Tomavo, S., B. Fortier, M. Soete, C. Ansel, D. Camus and J.F. Dubremetz,1991Toxoplasma gondii.]]>5937503753Uckert, W., W. Walther and U. Stein,19981998pp: 7-14pp: 7-14Zugelm, U. and S.H.E. Kaufmannm,1999121939