Research Journal of Microbiology1816-4935xxxx-xxxxScience International10.3923/jm.2006.152.159Debaryomyces nepalensis Isolated from Apple]]>GummadiSathyanarayana N.KumarD. Sunil2200612Yeast isolates GSP 101, 102 and 103 capable of producing both pectin lyase and pectate lyase were isolated from rotten apple. Among this, isolate GSP 101 exhibited higher levels of pectin lyase and pectate lyase. Based on 26S rDNA D1/D2 sequence analysis, the isolate was identified as Debaryomyces nepalensis (99.8% sequence identity). The isolate produced maximum 3.2 U mL-1 of pectin lyase and 2.3 U mL-1 of pectate lyase when grown in basal medium supplemented with pectin (5 g L-1). The presence of glucose in medium did not show any negative effect on production of pectic lyases suggesting that there is no catabolite repression. Among the tested naturally available pectic substances, lemon peel was best inducer and carbon source for the production of PL and PGL. This is the first report on production of pectin lyase and pectate lyase by Debaryomyces nepalensis.]]>Alana, A., I. Alkorta, J.B. Dorminguez, M.J. Llama and J.L. Serra,1990Penicillium italicum strain.]]>5637553759Albersheim, P.,19668628631Bruhlmann, F., K.S. Kim, W. Zimmerman and A. Fiechter,1994Actinomycetes for the degumming of ramie bast fibres.]]>6021072112Dave, B.A. and R.H. Vaughn,1971Bacillus pumilus.]]>108166174Gummadi, S.N. and D.S. Kumar,200527451458Satyanarayana, N.G. and T. Panda,200338987996Hayashi, K., Y. Inoue, M. Siga, S. Sato and R. Takano et al.,1997Pseudomonas marginalis MAFF 03-01173.]]>4513591363Henrissat, B., S.E. Heffron, M.D. Yoder, S.E. Lietzke and F. Jurnak,1995107963976Hepler, P.K. and R.O. Wayne,198536397439Hoondal, G., R. Tiwari, R. Tewari, N. Dahiya and Q. Beg,200259409418Kashyap, D.R., P.K. Vohra, S. Chopra and R. Tewari,200177215227Kashyap, D.R., S. Chandra, A. Kaul and R. Tewari,2000Bacillus sp. DT7.]]>16277282Manachini, P.L., C. Parini and M.G. Fortina,1998Aureobasidium pullalans LV 10.]]>10682685McCarthy, R.E., S.F. Kotarski and A.A. Salyers,1985Bacteroides thetaiotaomicron.]]>161493499Naidu, G.S.N. and T. Panda,199819355361Nakagawa, T., T. Miyaji, H. Yurimoto, Y. Sakai, N. Kato and M.A. Tomizuka,2000Candida boidinii.]]>6642534257Panda, T. and G.S.N. Naidu,2000234749Piccoli-Valle, R. H., F.J.V. Passos, I.V. Brandi, L.A. Peternelli and D.O. Silva,2003Penicillium griseoroseum.]]>38849854Singh, S.A., H. Plattnera and H. Diekmann,1999Bacillus sp.]]>25420425Soriano, M., P. Diaz and F.I.J. Pastor,200550114118Tanabe, H., I. Kobayashi and I. Akamatsu,1988Bacillus sp.]]>5218551856Whittaker, J.R.,19901990pp: 133-176pp: 133-176