Journal of Fisheries and Aquatic Science1816-49271996-0751Academic Journals Inc.10.3923/jfas.2015.35.44Panulirus polyphagus]]>FatihahS.N. SafiahJ. Abol-MunafiA.B. IkhwanuddinM. 12015101The study was aimed to determine the biochemical changes of total protein, glucose,
lactate dehydrogenase (LDH) and total lipid in the cryopreserved sperm of mud
spiny lobster, Panulirus polyphagus. The mean sperm viability, total protein,
glucose, LDH and lipid for control and cryopreserved sperm that stored in liquid
nitrogen from control, 6, 12, 24 h, 7, 30 and 60 days were 94.91±2.17%
(control), 80.58±1.33, 77.08±2.53, 76.49±3.10, 63.32±7.08,
62.93±3.72 and 60.91±10.78% (sperm viability), 21.44±3.52,
18.82±0.23, 17.79±0.80, 16.98±0.48, 16.65±0.07, 15.03±2.53
and 12.16±2.02 mg mL-1 (total protein), 0.28±0.012, 0.26±0.004,
0.20±0.035, 0.15±0.062, 0.11±0.036, 0.07±0.036 and
0.04±0.013 mg mL-1 (glucose), 72.65±7.88, 64.38±6.40,
64.24±4.80, 62.84±7.20, 62.11±3.84, 49.94±2.78, 38.31±3.29
IU L-1 (LDH) and 8.77±0.05, 4.87±0.25, 4.69±0.29,
4.37±0.02, 4.16±0.17, 2.87±0.29 and 2.83±0.26 mg mL-1
(total lipid). There were significant differences in sperm viability, total protein,
glucose, LDH and total lipid between cryopreserved periods (p<0.05). As a conclusion,
the sperm viability and biochemical changes of total protein, glucose, LDH and
total lipid were determined in the cryopreserved sperm for control, 6, 12, 24
h, 7, 30 and 60 days. The storage duration in P. polyphagus cryopreserved
sperm was also determined until 60 days and suitable for further breeding program.]]>Amaral, A., C. Paiva, M. Baptista, A.P. Sousa and J. Ramalho-Santos,201196848850Anchordoguy, T., J.H. Crowe, F.J. Griffin and W.H. Clark Jr.,1988Sicyonia ingentis.]]>25238243Aramli, M.S., M.R. Kalbassi and R.M. Nazari,2014Acipenser persicus (Borodin, 1897) semen.]]>65454Babiak, I., J. Glogowski, K. Goryczko, S. Dobosz, H. Kuzminski, J. Strzezek and W. Demianowicz,200156177192Bailey, J.,20122012Barnes, H. and J. Blackstock,197312103118Behlmer, S.D. and G. Brown,1984 Limulus polyphemus.]]>7193199Bhavanishankar, S. and T. Subramoniam,1997Scylla serrata (Forskal).]]>277326336Billard, R.,197814187198Blesbois, E., I. Grasseau and F. Seigneurin,2005129371378Ciereszko, A. and K. Dabrowski,199412357367Donoghue, A.M. and G.J. Wishart,200062213232Fatima, H., Z. Ayub, S.A. Ali and G. Siddiqui,2013Fenneropenaeus merguiensis and F. penicillatus (Crustacea: Decapoda).]]>37334347Fatihah, S.N., J. Safiah and A.B. Abol-Munafi and M. Ikhwanuddin,2014Panulirus polyphagus.]]>17937941Fatihah, S.N., J. Safiah, A.B. Abol-Munafi and M. Ikhwanuddin,2014Panulirus polyphagus).]]>1711241129Fraser, L.R. and P.J. Quinn,1981612535Garner, D.L. and M.H. Ehlers,1971°C on the disc electrophoretic patterns of ovine and bovine seminal proteins.]]>274352Hoppe, P.C.,1976in vitro.]]>153945Hughes, J.B.,1973Crassostrea virginica.]]>10342344Ikhwanuddin, M., S.N. Fatihah, A.H. Nurfaseha, M. Fathiah and M. Effenddy et al.,2014Panulirus polyphagus.]]>9113Jeyalectumie, C. and T. Subramoniam,1986Paratelphusa hydrodromous (Herbst).]]>46231236Jeyalectumie, C. and T. Subramoniam,1989Scylla serrata.]]>177247253Li, P., M. Hulak, P. Koubek, M. Sulc and B. Dzyuba et al., 2010Cyprinus carpio L.]]>74413423Lowry, O.H., N.J. Rosebrough, A.L. Farr and R.J. Randall,1951193265275Memon, A.J., A.D. Talpur, M.I. Khan, M.O. Fariddudin, J. Safiah, A.B. Abol-Munafi and M. Ikhwanuddin,2012Penaeus merguiensis).]]>1116881704Miki, K.,200765309325Niwa, K. and A. Iritani,1978in vitro.]]>53267271Nynca, J., H. Kuzminski, G.J. Dietrich, P. Hliwa and S. Dobosz et al.,2012Oncorhynchus mykiss, Walbaum).]]>77174183Partyka, A., W. Nizanski and E. Lukaszewicz,20107410191027Pickett, B.W. and R.J. Komarek,196447905908Sasikala, S. and B. Meena,2009Panulirus homarus.]]>919992003Stoss, J.,19831983pp: 305-350pp: 305-350Talbot, P. and S. Helluy,19951995pp: 528pp: 528Tsunoda, Y. and M.C. Chang,1975In vitro fertilization of rat and mouse eggs by ejaculated sperm and the effect of energy sources on in vitro fertilization of rat eggs.]]>1937986Urner, F. and D. Sakkas,199655917922Wang, Q., M. Misamore, C.Q. Jiang and C.L. Browdy,1995Penaeus vannamei: Dietary effects on sperm quality.]]>26261271Watson, P.F.,19957871891Williams, K.C.,2007202537Williams, A.C. and W.C.L. Ford,200122680695Yoo, B.Y., M.A. Ryan and A.J. Wiggs,1987Salmo salar L.) because of cryopreservation.]]>65913