Journal of Fisheries and Aquatic Science1816-49271996-0751Academic Journals Inc.10.3923/jfas.2009.103.110Oncorhynchus mykiss) Eggs]]>MousaviS.M. MirzargarS.S. Zadeh MousaviH. Ebrahim BaigiR. Omid KhosraviA. BahonarA. AhmadiM.R. 2200942The aim of this study was introducing a new herbal constitution
for malachite green in hatcheries. In this study, antifungal activities
of a new Combination of Essential Oils (CEO) from the herbs; Thymus
vulgaris, Salvia officinalis, Eucalyptus globulus and
Mentha piperita in Oncorhynchus mykiss eggs and its effects
on hatching rate in comparison with malachite green (a specific treatment
for the control of saprolegniasis) were studied. After fertilization,
eggs were transferred to incubators and then treated with five concentration
of the combined essential oils with concentrations: 10, 50, 100, 150 and
200 ppm, respectively and malachite green (1 ppm) using constant flow
treatment method for incubation period. One incubator was as control without
any treatment The water quality factors were controlled over the study
period. In the end of hatching rate, the mold infection and hatching rate
were calculated. The hatching percentage in five treatment groups of CEO
were 69.99% at 10 ppm, 63.61% at 50 ppm, 62.1% at 100 ppm, 60.53% at 150
ppm, 54.63% at 200 ppm, 60%. 83 at 1 ppm of malachite green group and
53.48% in control group, respectively. The results revealed significant
antifungal effects of the combined essential oils in comparison with malachite
green on fish eggs so that it could decrease mold infection and increase
hatching rate on concentration 10 ppm (p < 0.05). This indicated that
this combination of essential oils may be a promising antifungal agent
in aquaculture.]]>Adam, K., A. Sivropoulou, S. Kokkini, T. Lanaras and M. Arsenakis,1998Origanum vulgare subsp. hirtum, Mentha spicata, Lavandula angustifolia and Salvia fruticosa essential oils against human pathogenic fungi.]]>4617391745Bajpai, V.K., A. Rahman and S. Ch. Kang,2007262835Barnes, M.E., D.E. Ewing, R.J. Cordes and G.L. Young,1998Saprolegnia spp. during rainbow trout egg incubation.]]>606770Braga, P.C., M. Alfieri, M. Culici and M. Dal-Sasso,2007Candida albicans hyphae.]]>50502506Burt, S.,200494223253Carral, J.M., J.R. Perez, J.D. Celada, M. Saez-Royuela, P.M. Melendre and A. Aguilera,2004Austropotamobius pallipes Lereboullet.]]>372-373424430Cowan, M.M.,199912564582Davidson, P.M. and M.E. Parish,198943148155Denyer, S.P. and W.B. Hugo,19911991pp: 331-334pp: 331-334Duarte, M.C.T., G.M. Figueira, A. Sartoratto, V.L.G. Rehder and C. Delarmelina,2005Candida activity of Brazilian medicinal plants.]]>97305311Forneris, G., S. Bellardi, G.B. Palmegiano, M. Saroglia, B Sicuro., L. Gasco and I. Zoccarato,2003221157166Gill, A.O., P. Delaquis, P. Russo and R.A. Holley,2002738392Gonzales, A.G., T. Abad, I.A. Jiminez, A.G. Ravelo and J.G.L.Z. Aguiar et al.,1989Salvia Species (Lamiaceae).]]>17293296Hussein, M.M.A. and K. Hatai,2002Saprolegnia species associated with outbreaks of salmonid saprolegniosis in Japan.]]>6810671072Iscan, G., N. Kirimer, M. Kurkcuoglu, K.H.C. Baser and F. Demirci,2002Mentha piperita essential oils.]]>5039433946Kitancharoen, N., A. Yamamoto and K. Hatai,199738375378Lee, S.H., K.S. Chang, M.S. Su, Y.S. Huang and H.D. Jang,20061815471554Lilley, I.H. and V. Inglis,1997Aphanomyces invaderis and other saprolegniaceous fungi.]]>28461469Lima, C.F., F. Carvalho, E. Fernandes, M.L. Bastos, P.C. Santos-Gomes, M. Fernandes-Ferreira and C. Pereira-Wilson,2004Salvia officinalis on freshly isolated rat hepatocytes.]]>18457465Marino, M., C. Bersani and G. Comi,2001Lamiaeae and Compositae.]]>67187195Mishra, A.K. and N.K. Dubey,19946011011105Nilsen, P.V. and R. Rios,200060219229Noga, E.J.,19961st Edn.,pp: 163-164pp: 163-164Olah, J. and J. Farkas,1978Saprolegnia and Achlya parasiticav on fish.]]>13273288Olesen, P.T., J.C. Larsen and A. Schnipper,2007172S198S199Pinto, E., L. Riberio-Saluerio, C. Cavaleiro, A. Palmeria and M. Jose-Goncalves,2007In vitro susceptibility of some species of yeasts and filamentous fungi to essential oils of Salvia officinalis.]]>26135141Pina-Vaz, C., A.G. Rodfigues, E. Pinto, H.S. Costa-de-Oiiveira and C. Tavares et al.,2004Thymus oils and their major compounds.]]>187378Post, G.,1987pp: 288pp: 288Schulz, H., M. Baranska, H.H. Belz, P. Rosch, M.A. Strehle and J. Popp,2004358186Rohani, M.S., H.A. Ebrahim-Zadeh-Mousavi, A.R. Khosravi, B. Mokhayer, S.S. MIR-Zargar and Y. Mehrabi,2006Geranium herbarum escence application in control of fungal contamination in rainbow trout eggs.]]>61269272Smith-Palmer, A., J. Stewart and L. Fyfe,199826118122Stammati, A., C. Nebbia, I. Angelis, A. Giuliano-Albo and M. Carletti et al.,200519853858Stenhagen, E., S. Abrahamson and F. Mclafery,19741st Edn.,Sudova, E., J. Machova, Z. Svobodova and T. Vesely,200752527539Summerfelt, S.T., J.A. Hankins, A.L. Weber and M.D. Durant,19971585767Tassou, C., K. Koutsoumanis and G.J.E. Nychas,2000Salmonella enteritidis and Staphylococcus aureus in nutrient broth by mint essential oil.]]>33273280Valero, M. and M.C. Salmeron,2003Bacillus cereus in tyndallized carrot broth.]]>857381