Journal of Environmental Science and Technology1994-78872077-2181Asian Network for Scientific Information10.3923/jest.2013.130.137PantGeetika MistrySandeep Kumar SibiG. 3201363DDT (dichlorodiphenyltrichloroethane), one of the organochlorine pesticides
was widely used in agriculture and healthcare. Though its usage is banned in
most of the countries, DDT residues cause varying negative effects through bioaccumulation
and bioamagnification. Biodegradation is an potential method to detoxify the
recalcitrant compounds and this study is an investigation to isolate and characterize
the p, p-DDT degrading bacteria from DDT contaminated soil. An p, p-DDT [1,1,1-trichloro-2,2-bis(4-chlorophenyl)ethane]
degrading bacterium was isolated and identified by 16S rRNA studies. The role
of temperature, pH and DDT concentration in the degrading ability of the isolate
has also been investigated. Based on the analysis of the phenotype, biochemical
characteristics and 16S rRNA, the strain was identified to belong to the bacterial
genera Bacillus and was named as Bacillus strain GSS. The isolate
had an optimum pH of 7.0 at 35°C and was able to degrade DDT at a wide range
of concentrations with complete degradation of the DDT (10 mg L-1)
and 89.3% of 15 mg L-1 in 120 h, whereas 100% degradation of 5 mg
L-1 concentration was observed within 48 h. Significant degradation
was observed at 72 h and 96 h for 15 and 20 mg L-1, respectively.
At the end of 120 h, 73% of 20 mg L-1 and 34% of 25 mg L-1
was recorded by the isolate Bacillus strain GSS.]]>Ahuja, R. and A. Kumar,2004Alcaligenes denitrificans ITRC-4 under aerobic and anaerobic conditions.]]>466569Singh, A. and O.P. Ward,2004pp: 36-37pp: 36-37Baker, G.C., J.J. Smith and D.A. Cowan,200355541555Corona-Cruz, A., G. Gold-Bouchot, M. Gutierrez-Rojas, O. Monroy-Hermosillo and E. Favela,199963219225Cutright, T.J. and Z. Erdem,201293945Diaz, E.,2004711391143Dua, M., A. Singh, N. Sethunathan and A. Johri,200259143152Fang, H., B. Dong, H. Yan, F. Tang and Y. Yu,2010184281289Frank, J.A., C.I. Reich, S. Sharma, J.S. Weisbaum, B.A. Wilson and G.J. Olsen,20087424612470Gao, B., W.B. Liu, L.Y. Jia, L. Xu and J. Xie,2011Alcaligenes sp. strain DG-5 capable of degrading DDTs under aerobic conditions.]]>46257263Howard, P.H.,19911991pp: 684pp: 684Barragan-Huerta, B.E., C. Costa-Perez, J. Peralta-Cruz, J. Barrera-Cortes, F. Esparza-Garcia and R. Rodriguez-Vazquez,200759239244Juhasz, A.L. and R. Naidu,2000a]pyrene.]]>455788Kamanavalli, C.M. and H.Z. Ninnekar,2004Pseudomonas species.]]>481013Kannan, K., S. Tanabe, A. Ramesh, A. Subramanian and R. Tatsukawa,199240518524Kumar, S., K.G. Mukerji and R. Lal,199622126Menone, M.L., J.E.A. de Moreno, V.J. Moreno, A.L. Lanfranchi, T.L. Metcalfe and C.D. Metcalfe,200140355362Mwangi, K., H.I. Boga, A.W. Muigai, C. Kiiyukia and M.K. Tsanuo,20104185196Nadeau, L.J., F.M. Menn, A. Breen and G.S. Sayler,1994Alcaligenes eutrophus A5.]]>605155Nadeau, L.J., G.S. Sayler and J.C. Spain,1998Alcaligenes eutrophus A5.]]>1714449Nawab, A., A. Aleem and A. Malik,2003Pseudomonas strains.]]>884146Patnaik, P.,2003Pages: 1059Pages: 1059Kuhad, R.C., A.K. Johri, A. Singh and O.P. Ward,20042004pp: 35-54pp: 35-54Schmidt, T.M., E.F. Delong and N.R. Pace,199117343714378Singh, B.K., R.C. Kuhad, A. Singh, R. Lal and K.K. Tripathi,199919197225Singh, D.K.,2008483540Smith, A.G.,19911991pp: 731-915pp: 731-915Thomas, J.E., L.T. Ou and A. All-Agely,20082008pp: 55-69pp: 55-69Vaccari, D.A., P.F. Strom and J.E. Allema,2006Wang, G., J. Zhang, L. Wang, B. Liang, K. Chen, S. Li and J. Jiang,2010Pseudoxanthomonas sp. Wax.]]>41431438Wang, T.Y., Y.L. Lu, R.W. Dawson, Y.J. Shi, H. Zhang and Y. Xing,200641309321Zhang, C. and G.N. Bennett,200567600618