Journal of Entomology1812-56701812-5689Asian Network for Scientific Information10.3923/je.2021.19.28Mentha piperita Extract on Culex quinquefasciatus Larvae (Diptera: Culicidae)]]>SubaharRizal Kamil Putra KadarusmanAdib Fatmawaty SaharNurhuda WinitaRawina SusantoLisawati Yulhasri LubisNadar S. Mulyati Eko FirmansyahNurhadi 12021181Background and Objective:Mentha piperita (peppermint) contains terpenoids (essential oils) that can destroy the digestive tracts and nervous systems of insects. The objective of the study was to evaluate the effect of low concentrations of the crude leaf extract of M. piperita on C. quinquefasciatus larvae through histopathological midgut changes and decreased immunoreactivity of octopamine and tyramine. Materials and Methods: The phytochemical analysis of the extract was performed using Gas Chromatography-Mass Spectrometry (GC-MS). Culex quinquefasciatus larvae were exposed to different concentrations of the extract. Histopathological changes in the midguts of the larvae were tested by histopathological examination and the immunoreactivity of octopamine and tyramine was measured using an immunohistochemical method. Results: Terpenoids were major components of the M. piperita crude leaf extract. At 24 hrs, the LC50 and LC90 values were 2.56 and 6.64 ppm, respectively. The extract caused several histopathological midgut changes, including fragmented food boluses, deformed epithelial cells, disintegrated epithelial layers, damaged microvilli and broken peritrophic membranes. Octopamine and tyramine were detected in the midgut, but their immunoreactivity had decreased. Conclusion:Mentha piperita has the potential to eradicate the population of C. quinquefasciatus as an alternative insecticide.]]>Müller, R., F. Reuss, V. Kendrovski and D. Montag,20192019pp: 67-90pp: 67-90Ramirez, B.,20172017Soh, S. and J. Aik,2021Culex mosquito vectors for West Nile Virus and other flaviviruses: A time-series analysis of rainfall and temperature dependence in Singapore.]]>2021Negi, C., P. Verma,2018Culex quinquefasciatus: Southern house mosquito.]]>415631566Impoinvil, D.E., S. Ahmad, A. Troyo, J. Keating and A.K. Githeko et al.,200783196212Hierlihy, C., L. Waddell, I. Young, J. Greig, T. Corrin and M. Mascarenhas,20192019Antonio, C.A.T., A.N.C. Bermudez, K.L. Cochon, M.S.G.L. Reyes and C.D.H. Torres et al.,2020222S726S731Moyes, C.L., J. Vontas, A.J. Martins, L.C. Ng and S.Y. Koou et al.,2017Aedes vectors of arboviruses infecting humans.]]>2017Delannay, C., D. Goindin, K. Kellaou, C. Ramdini, J. Gustave and A. Vega-Rúa,2018Culex quinquefasciatus populations from Guadeloupe (French West Indies) and associated mechanisms.]]>2018Rai, P., M. Bharati, A. Subba and D. Saha,2019Culex quinquefasciatus Say from northern region of West Bengal, India.]]>2019Dias, C.N. and D.F.C. Moraes,2014Aedes aegypti L. (Diptera: Culicidae) larvicides: Review.]]>113565592Singh, P. and A.K. Pandey,2018Mentha as biopesticide: A review.]]>2018Kalaivani, K., S. Senthil-Nathan and A.G. Murugesan,2012Aedes aegypti L. (Diptera: Culicidae).]]>11012611268Plata-Rueda, A., G. Da Silva Rolim, C.F. Wilcken, J.C. Zanuncio, J.E. Serrão and L.C. Martínez,2020Sitophilus granarius.]]>2020Kumar, S., N. Wahab and R. Warikoo,2011Mentha piperita essential oil against dengue fever mosquito Aedes aegypti L.]]>18588Pathak, N., P.K. Mittal, O.P. Singh, D.V. Sagar and P. Vasudepan,2000Anopheles stephensi (Liston), Culex quinquefsaciatus (Say) and Aedes aegypti (L).]]>425355Kostyukovsky, M., A. Rafaeli, C. Gileadi, N. Demchenko and E. Shaaya,20025811011106Jankowska, M., J. Rogalska, J. Wyszkowska and M. Stankiewicz,20182018Shahzad, R., A.L. Khan, M. Waqas, I. Ullah and S. Bilal et al.,2019Bacillus amyloliquefaciens RWL-1 during methanol utilization.]]>2019Subahar, R., A. Achmadsyah, M. Yasmine, L. Susanto, Fatmawaty and R. Winita,2019Aedes aegypti (Diptera: Culicidae) mosquitoes, dengue vector.]]>41520Sharma, G., K. Kumar, A. Sharma and V. Agrawal,2012Artemisia annua leaf extracts and artemisinin against larvae of Culex quinquefasciatus and Culex tritaeniorhynchus.]]>28317319Lemos, A., F. Adam, K. Moura, L. Moraes and O. Silva,201822115Ramos-Vara, J.A.,20172017pp: 115-128pp: 115-128Fan, C., D. Zhang and C.H. Zhang,201167213224Huang, Y.T. and T. Cai,201672563574Kalemba, D. and A. Synowiec,2019Mentha piperita and Mentha arvensis.]]>2019Samber, N., A. Khan, A. Varma and N. Manzoor,2015Mentha piperita essential oil and its major components.]]>5314961504Saeidi, K. and S. Mirfakhraie,2017Mentha piperita L. essential oil against the cowpea seed beetle Callosobruchus maculatus F. (Coleoptera: Bruchidae).]]>49127134Tong, W.Y.,20132013pp: 2733-2759pp: 2733-2759Isah, M.B., N. Tajuddeen, M.I. Umar, Z.A. Alhafiz, A. Mohammed and M.A. Ibrahim,201859227250Singh, R., M.A. Shushni and A. Belkheir,2015Mentha piperita L.]]>8322328Sies, H.,199782291295Yu, X., Q. Sun, B. Li, Y. Xie and X. Zhao et al.,2015Bombyx mori.]]>30452460Roeder, T.,200550447477Damrau, C., N. Toshima, T. Tanimura, B. Brembs and J. Colomb,2018Drosophila.]]>2018Maddala, V.K.S.,2019102141152Saeidi, K. and B. Hassanpour,2014Mentha piperita L. and Mentha pulegium L. essential oils on nutritional indices of Plodia interpunctella Hübner (Lepidoptera: Pyralidae).]]>461317Iwuagwu, M.O., P.E. Etusim, N.C. Emmanuel, J.C. Igwe, V.O. Nwaugo and R.A. Onyeagba,20208721