Journal of Entomology1812-56701812-5689Asian Network for Scientific Information10.3923/je.2016.72.83El-BassionyMohamed N. MahfouzHatem M. HusseinAhmed S. El-HamamyMahmoud M. DaimMohamed M. Abdel BufoSabino A. 32016133The present study aimed to evaluate the tumor growth inhibiting effects of bee venom in rats (in vivo) and in tumor cell cultures (in vitro). Growth inhibitory effect of bee venom (in vitro) administered in various concentrations (0, 0.01, 0.1, 1, 10 and100 μg mL1) to eight different types of cancer cell lines were estimated by counting their viable cell number after 72 h of treatment. Results indicated that the growth inhibition by bee venom was dose dependent in all cell lines. The highest death cell was observed in HEPG2 (liver cancer), A549 (lung cancer) and HEP-2C (larynx cancer) cell lines treated with bee venom, while the lowest one was occurred with HCT116 cell line (colon cancer). Intraperitoneally administration of bee venom (0.28 and 0.56 mg kg1) to rats (in vivo) significantly reduced the body weight, ascites tumor volume, Packed Cell Volume (PCV), viable tumor cell, tumor cell count number and increased Mean Survival Time (MST). Aspartate aminotransferease (AST) and alanine aminotransferease (ALT), alkaline phosphatase (ALP) and total proteinand albumin levels significantly (p≤0.05) decreased in ehrlich ascites carcinoma bearing rats treated groups with 0.28 and 0.56 mg kg1 of bee venom (G5, G6) and 5-fluoruracil (G4). Histopathological investigation of peritoneum, liver, kidney and lung was performed. The results indicated that the studied bee venom can be used as effective anticancer agent.]]>Abu-Zeid, M., H. Hori, H. Nagasawa, Y. Uto and S. Inayama,200023195198Alizadehnohi, M., M. Nabiuni, Z. Nazari, Z. Safaeinejad and S. Irian,201232227Bancroft, J.D. and A. Stevens,19964th Edn.,Pages: 766Pages: 766Baskar, R., K.A. Lee, R. Yeo and K.W. Yeoh,20129193199Coles, E.H.,19864th Edn.,Pages: 486Pages: 486Doumas, B.T., W.A. Watson and H.G. Biggs,1971318796Ellefson, R.D. and W.T. Caraway,19761976pp: 506-510pp: 506-510Frankenberg, L.,197913311312Gajski, G. and V. Garaj-Vrhovac,201336697705Gajski, G., T. Cimbora-Zovko, S. Rak, M. Rozman, M. Osmak and V. Garaj-Vrhovac,20143413321341Garrison, R.N., R.H. Galloway and L.S. Heuser,198742126132Grant, G.H., L.M. Silverman and R.H. Christenson,19871987pp: 312-316pp: 312-316Hong, S.J., G.S. Rim, H.I. Yang, C.S. Yin and H.G. Koh et al.,2005463945Jang, M.H., M.C. Shin, S. Lim, S.M. Han and H.J. Park et al.,20039195104Kind, P.R.N. and E.J. King,19547322326Liu, S., M. Yu, Y. He, L. Xiao and F. Wang et al.,20084719641973Merit, B.M.,20062006Orsolic, N., P. Josipovic and I. Basic,2009in vivo and in vitro.]]>6115Orsolic, N.,201231173194Park, J.H., Y.J. Jeong, K.K. Park, H.J. Cho and I.K. Chung et al.,201029209215Premratanachai, P. and C. Chanchao,20144337344Putz, T., R. Ramoner, H. Gander, A. Rahm, G. Bartsch and M. Thurnher,20065513741383Raghuraman, H. and A. Chattopadhyay,200727189223Reitman, S. and S. Frankel,1957285663Rock, R.C., W.G. Walker and C.D. Jennings,19871987pp: 669-704pp: 669-704Rybak, M., J. Muszynska, P. Skubida and J. Marcinkowski,199539223231SAS.,1996Siegel, R., D. Naishadham and A. Jemal,2012621029Ip, S.W., H.C. Wei, J.P. Lin, H.M. Kuo and K.C. Liu et al.,200828833842Skehan, P., R. Storeng, D. Scudiero, A. Monks and J. McMahon et al.,19908211071112Slaninova, J., V. Mlsova, H. Kroupova, L. Alan and T. Tumova et al.,2012331826Son, D.J., J.W. Lee, Y.H. Lee, H.S. Song, C.K. Lee and J.T. Hong,2007115246270Young, D.S.,19903rd Edn.,pp: 6-12pp: 6-12