Journal of Biological Sciences1727-30481812-5719Asian Network for Scientific Information10.3923/jbs.2019.137.142Hibiscus sabdariffa L.) Calyx Extracts against Jurkat T-Lymphoblastic Leukaemia Cells]]>AwangNormah AzizNurul Amalina Abd MengChan Kok KamaludinNurul Farahana MohamadRapidah YousofSyaidatul Akmal Mohd 22019192Background and Objective: Plant derivative compounds have been widely used in in vivo and in vitro studies as potential anticancer agents. Several studies demonstrated that Roselle (Hibiscus sabdariffa L.) possessed an anticancer effect against few cancer cell lines. However, study on their cytotoxicity against lymphoblastic leukaemia cell lines still remains unclear. Therefore, this study was conducted to assess the cytotoxic effect of Hibiscus sabdariffa L. (Roselle) calyx water and ethanol extracts against Jurkat T-lymphoblastic leukaemia cell lines. Materials and Methods: A powder of Roselle calyx was extracted using different types of solvent via ultra-sonication extraction method. The extracts obtained were then assessed for their cytotoxicity against Jurkat cells using (3-[4, 5-dimethylthiazol-2-yl]-2, 5-diphenyl tetrazolium bromide) (MTT) assay upon 24, 48 and 72 h treatment. Results: Water solvent gave the highest percentage of yield followed by ethanol solvent, with the least extract came from hexane solvent. The water and ethanol extracts of Roselle calyx gave no IC50 values in MTT assay yet demonstrated a decrease in cell viability of Jurkat cells in dose and time-dependant manner. The viability of treated cells at concentrations of 1.0, 0.5, 0.25 and 0.125 mg mL1 of water extract gave a statistical difference (p<0.05) compared to untreated cells. Meanwhile, no statistical differences (p = 0.610) between cell treated with Roselle ethanol extract at any concentrations with negative control. Conclusion: This study indicated that water and ethanol extracts of Roselle calyx were not-toxic towards Jurkat cells. This could be due to the selectivity of phytochemical in which their cytotoxic effect depends on human cancer types. However, those extracts can be further studied for their other potential such as chemo-preventive agent towards Jurkat cell line.]]>National Cancer Institute,20172017Inaba, H., M. Greaves and C.G. Mullighan,201338119431955Huang, T.T. and K.K. Ness,20112011Adams, M.J. and S.E. Lipshultz,200544600606Printz, C.,201011625072507Roja, G. and P.S. Rao,2000771102Ochani, P.C. and P. D'Mello,2009Hibiscus sabdariffa Linn. leaves and calyces extracts in rats.]]>47276282Ali, B.H., N. Al Wabel and G. Blunden,2005Hibiscus sabdariffa L.: A review.]]>19369375Da-Costa-Rocha, I., B. Bonnlaender, H. Sievers, I. Pischel and M. Heinrich,2014Hibiscus sabdariffa L.-a phytochemical and pharmacological review.]]>165424443Chang, Y.C., H.P. Huang, J.D. Hsu, S.F. Yang and C.J. Wang,2005205201212Hou, D.X., X. Tong, N. Terahara, D. Luo and M. Fujii,2005Hibiscus anthocyanin, induces apoptosis in human leukemia cells through reactive oxygen species-mediated mitochondrial pathway.]]>440101109Lin, H.H., J.H. Chen, W.H. Kuo and C.J. Wang,2007Hibiscus sabdariffa L. on human gastric carcinoma cells through apoptosis induction and JNK/p38 MAPK signaling activation.]]>1655975Nafizah, A.H.N., S.B. Budin, A.H. Zaryantey, A.R. Mariati and R.L. Santhana et al.,2017Hibiscus sabdariffa Linn supplementation improves liver morphology in streptozotocin induced diabetic rats.]]>181320Mohamed, J., S.W. Shing, M.H.M. Idris, S.B. Budin and S. Zainalabidin,2013Hibiscus sabdariffa L. UKMR-2) against red blood cell membrane oxidative stress in rats with streptozotocin-induced diabetes.]]>6813581363Ramalingam, A., S.B. Budin, Y.C. Lim, Y.N.L. Si and S. Zainalabidin,20164511311137Si, L.Y.N., S.A.M. Ali, J. Latip, N.M. Fauzi, S.B. Budin and S. Zainalabidin,2017191157165Abdul Hamid, Z., W.H.L. Lin, B.J. Abdalla, O.B. Yuen and E.S. Latif et al.,2014Hibiscus sabdariffa L. (Roselle) in maintenance of Ex Vivo murine bone Marrow-derived hematopoietic stem cells.]]>2014Annegowda, H.V., R. Bhat, L. Min-Tze, A.A. Karim and S.M. Mansor,201249510514Khaghani, S., F. Razi, M.M. Yajloo, M. Paknejad, A. Shariftabrizi and P. Pasalar,2011Hibiscus sabdariffa aqueous extract against MCF-7 human breast cancer cell line.]]>2394400Snyder, L.R.,197492223230Khalaphallah, R. and W.S. Soliman,20144292296Pin, K.Y., A.L. Chuah, A.A. Rashih, M.P. Mazura, J. Fadzureena, S. Vimala and M.A. Rasadah,2010Piper betle) from solvents with different polarities.]]>22448455Abdul Razak, M.F.B., P.K. Yong, ZM. Shah, L.C. Abdullah, S.S. Yee and I.T.C.S. Yaw,2012Orthosiphon stamineus leaves.]]>1212071210Liu, T.Z., J.T. Cheng, S.J. Yiin, C.Y. Chen, C.H. Chen, M.J. Wu and C.L. Chern,200846321327Crawford, D.R. and K.J. Davies,19941022528Bonilla-Porras, A.R., M. Jimenez-Del-Rio and C. Velez-Pardo,20112011Sak, K.,20148122146Mohd-Esa, N., F.S. Hern, A. Ismail and C.L. Yee,2010Hibiscus sabdariffa L.) extracts and potential exploitation of the seeds.]]>12210551060Rajab, N.F., S.M. Musa, M.A. Munawar, L.L. Mun, H.K. Yen, F.W. Ibrahim and C.K. Meng,2016Hibiscus sabdariffa Linn. (Roselle) on lipopolysaccharides-induced microglia and neuroblastoma cells.]]>14111117D'Andrea, G.M.,200555319321Lawenda, B.D., K.M., Kelly, E.J. Ladas, S.M. Sagar, A. Vickers and J.B. Blumberg,2008100773783Salganik, R.I.,200120464472