Journal of Biological Sciences1727-30481812-5719Asian Network for Scientific Information10.3923/jbs.2017.1.10Pseudomonas sp., LS3K Isolated from Odorous Region of Poultry Farm]]>Agus FitriyantoNanung WinartiAtik Akbar ImaraFatih ErwantoYuny HayakawaTakashi NakagawaTomoyuki 12017171Background and Objective: A proper management processes for safe and efficient handling of animal manure is important to minimize the possibilities of global environmental problem due to the emission of unpleasant odor. This study was performed to identify strains bacteria screened from the soil in the odorous region of apoultry format Yogyakarta city, Indonesia, to evaluate their capability for growing in ammonium and animals urine high content medium and also to investigate the ability to reduce ammonium concentration from the liquid medium. Materials and Methods: The strain is originally obtained from soil sample collected at ammonia high-emitted of chicken hen production area at tropical country Indonesia. The isolation was using a 1/100 nutrient agar with high concentration of (NH4)2SO4 as an organic ammonium stressor. Selected strain was then identified based on morphology and biochemical identifications, as well as molecular identification. The reduction of ammonium was measured from cultured medium using nessler reagent spectroscopically. Results: High ammonium sulfate-responsive, strain LS3K was successfully isolated and identified based on morphology, physiology and biochemical identifications, as well as molecular identification based on 16S rRNA sequence. Based on the morphology, physiological observation and the 16S rRNA gene sequence, strain LS3T was identified as Pseudomonas spp. The content of DNA G+C of the strain was 55.2%. The 16S rRNA gene sequence analysis demonstrated that the isolate is showing high similarity to species of Pseudomonas tolaasii. The cells of strain LS3K are short rods, aerobic and motile. Colonies (l-2 mm in diameter) on plate culture are yellowish, circular and smooth. This bacterium has an optimal growth temperature 30°C. Conclusion: Strain LS3K can tolerate ammonium at 756.77 mM and has the ability for growing in animals manure contained a medium that should make it applicable for use in animal waste handling systems as well as for enhancing the capability of deodorization. Ammonium was oxidized 5.648 mg L1 by strain LS3K during 30 h cultivation period.]]>Chadwick, D., S. Sommer, R. Thorman, D. Fangueiro, L. Cardenas, B. Amon and T. Misselbrook,2011166514531Posmanik, R., A. Gross and A. Nejidat,201462140147Elwinger, K. and L. Svensson,199664197208Xie, K., X. Jia, P. Xu, X. Huang and W. Gu et al.,20121207077Adrizal, P.H. Patterson, R.M. Hulet, R.M. Bates and D.A. Despot et al.,200817398411McCrory, D.F. and P.J. Hobbs, 200130345355Satoh, K., R. Takizawa, M. Sarai, N. Sato, R. Takahashi and T. Tokuyama,200498207210Zeng, Y., A. De Guardia, C. Ziebal, F.J. De Macedo and P. Dabert,2012110144152Ndegwa, P.M., A.N. Hristov, J. Arogo and R.E. Sheffield,2008100453469Zhang, J., P. Wu, B. Hao and Z. Yu,2011Pseudomonas stutzeri YZN-001.]]>10298669869Zeng, G., J. Zhang, Y. Chen, Z. Yu and M. Yu et al.,201110290269032Ma, Y., L. Wang and L. Qian,200818679684Sauder, L.A., K. Engel, J.C. Stearns, A.P. Masella, R. Pawliszyn and J.D. Neufeld,20112011Zhang, Q.L., Y. Liu, G.M. Ai, L.L. Miao, H.Y. Zheng and Z.P. Liu,2012Bacillus methylotrophicus strain L7.]]>1083544Joo, H.S., M. Hirai and M. Shoda,2005Alcaligenes faecalis.]]>27773778Hooijmans, C.M., S.G.M. Geraats, E.W.J. van Niel, L.A. Robertson, J.J. Heijnen and K.C.A.M. Luyben,1990Thiosphaera pantotropha using measurement and modeling of oxygen profiles.]]>36931939Zhao, B., Q. An, Y.L. He and J.S. Guo,20122O and N2 production during heterotrophic nitrification by Alcaligenes faecalis strain NR.]]>116379385Selvam, A., Z. Zhao, Y. Li, Y. Chen, K. Leung and J. Wong,20133424332441Sonoki, T., T. Furukawa, K. Jindo, K. Suto, M. Aoyama and M.A. Sanchez‐Monedero,201353617621Lin, S.Y., Y.C. Liu, A. Hameed, Y.H. Hsu, W.A. Lai, F.T. Shen and C.C. Young,2013Azospirillum fermentarium sp. nov., a nitrogen-fixing species isolated from a fermenter.]]>6337623768Kuykendall, L.D.,20052005pp: 438-442pp: 438-442Ausubel, F.M., R. Brent, R.E. Kingston, D.D. Moore, J.G. Seidman, J.A. Smith and K. Struhl,2003Vol. 1,Mackie, R.I., O.G. Stroot and V.H. Varel,19987613311342Ohta, Y. and M. Ikeda,197836487491Low, E.W. and H.A. Chase,19993311191132Sund, J.L., C.J. Evenson, K.A. Strevett, R.W. Nairn, D. Athay and E. Trawinski,200130648655Spieck, E., E. Bock and H.P. Koops,20052005Liao, C.M. and D.S. Bundy,19942912191249Bourque, D., J.G. Bisaillon, R. Beaudet, M. Sylvestre, M. Ishaque and A. Morin,198753137141Jolicoeur, P. and A. Morin,1987Acinetobacter calcoaceticus strains degrading the volatile fatty acids of swine wastes.]]>19133140Zhu, J.,20007893106