Journal of Biological Sciences1727-30481812-5719Asian Network for Scientific Information10.3923/jbs.2014.327.331Nur AkmalM.R. Intan ZarinaZ.A. RohayaM.A.W. SahidanS. ZaidahZ.A. Shahrul HishamZ.A. 42014144Studies have proven that dental pulp contains cells with stem cell activity. Although mice are widely used as a model organism, murine dental pulp stem cells (mDPSCs) are still poorly characterized. In this study, we aim to provide a modified and cost-effective method for isolating mDPSCs and to characterize the isolated cells using molecular markers. Mice incisors dental pulps were digested in collagenase 1A and the cell suspensions cultured in α-MEM supplemented with 20% (v/v) fetal bovine serum and 1% (v/v) penicillin-streptomycin. The morphology of the cells was observed and passage 4 cells were subjected to molecular characterization using RT-PCR. We found that the freshly isolated dental pulp cells through enzymatic dissociation method showed a heterogeneous cell morphology which became more homogeneous following cell passage. Semi-quantitative RT-PCR showed analysis that the murine incisors DPSCs are Cd105+, Cd13+, Cd29+, Cd146+, Cd166+, Cd90low, Cd73low, Cd105¯ and Cd34¯. Based on these findings, we concluded that dental pulp stem cells from murine incisor could be easily isolated through the method described and the incisors dental pulp could be a source of mesenchymal stem cells. Further studies are required to explore the differentiation potential of the isolated mDPSC in vitro.]]>Atari, M., M. Barajas, F. Hernandez-Alfaro, C. Gil and M. Fabregat et al.,20112610571070Bakopoulou, A., G. Leyhausen, J. Volk, A. Tsiftsoglou, P. Garefis, P. Koidis and W. Geurtsen,2011in vitro osteo/odontogenic differentiation potential of human dental pulp stem cells (DPSCs) and stem cells from the apical papilla (SCAP).]]>56709721Balic, A., H.L. Aguila, M.J. Caimano, V.P. Francone and M. Mina,20104616391651Huang, G.T.J., W. Sonoyama, J. Chen and S.H. Park,2006In vitro characterization of human dental pulp cells: Various isolation methods and culturing environments.]]>324225236Iohara, K., L. Zheng, M. Ito, A. Tomokiyo, K. Matsushita and M. Nakashima,20062424932503Kaneko, T., U. Arayatrakoollikit, Y. Yamanaka, T. Ito and T. Okiji,2013351425432Korbie, D.J. and J.S. Mattick,2008314521456Lee, J.K. and J.H. Lee,201036715Mao, J.J. and D.J. Prockop,201211291301Morsczeck, C., F. Vollner, M. Saugspier, C. Brandl, T.E. Reichert, O. Driemel and G. Schmalz,2010in vitro.]]>14433440Snyder, B., P.H. Cheng, J. Yang, S.H. Yang, A. Huang and A. Chan,20112011Sonoyama, W., Y. Liu, D. Fang, T. Yamaza and B.M. Seo et al.,20062006Souza, L.M.D., J.D. Bittar, I.C.R.D. Silva, O.A.D. Toledo, M.D.M. Birigido and M.J. Pocas-Fonseca,20109427433Tecles, O., P. Laurent, S. Zygouritsas, A.S. Burger, J. Camps, J. Dejou and I. About,200550103108Tziafas, D. and K. Kodonas,201036781789Volponi, A.A., Y. Pang and P.T. Sharpe,201020715722Waddington, R.J., S.J. Youde, C.P. Lee and A.J. Sloan,2008189268274Yahyouche, A., X. Zhidao, J.T. Czernuszka and A.J.P. Clover,20117278286Yildirim, S.,20132013pp: 17-24pp: 17-24