Journal of Biological Sciences1727-30481812-5719Asian Network for Scientific Information10.3923/jbs.2014.149.153Abu-ZaitonAhmed Saber22014142Metformin is a commonly used anti-diabetic chemical. Underlying mechanisms are
not fully understood if its actions are directed towards insulin receptors downstream
events. In vitro expansion and proliferation of fibroblast stem cells depend on
the presence of certain environmental factors among which is oxygen. The major
goal of this study was to investigate the effect of Metformin on the proliferation
of skin derived stem cells in case of normoxia and hypoxia. The results suggest
that fibroblast stem cells proliferation is largely affected by high dose (100
μg mL-1) of Metformin. Obtained data indicated that Metformin
significantly inhibited cell survival by a dose-dependent manner in three doses:
Metformin 10, 50 and Metformin 100 μg mL-1. Metformin decreased
cell survival in dose 100 μg mL-1 more than other two doses, also
the experiment pointed to a significant effect of short-term low oxygen (5% O2)
treatment on fibroblast proliferation combined with data that fibroblast exposed
to 20% oxygen tension exhibited enhanced fibroblast proliferation and viability.
By MTT assay results, it has been noticed that no significant changes in the proliferation
of fibroblast during the cell culturing both on the first and the third day after
metformin administration.]]>Bao, B., Z. Wang, S. Ali, A. Ahmad and A.S. Azmi et al.,20125355364Csete, M.,2005104918Domm, C., M. Schunke, K. Christesen and B. Kurz,2002101322Evans, J.M., L.A. Donnelly, A.M. Emslie-Smith, D.R. Alessi and A.D. Morris,200533013041305Fries, K.M., T. Blieden, R.J. Looney, G.D. Sempowski, M.R. Silvera, R.A. Willis and R.P. Phipps,199472283292Grayson, W.L., F. Zhao, B. Bunnell and T. Ma,2007358948953Grimshaw, M.J. and R.M. Mason,2000in vitro depends upon oxygen tension.]]>8386392Hirsch, H.A., D. Iliopoulos, P.N. Tsichlis and K. Struhl,20096975077511Kurz, B., C. Domm, M. Jin, R. Sellckau and M. Schunke,20041012771286Haniffa, M.A., M.P. Collin, C.D. Buckley and F. Dazzi,200994258263Mizuno, S. and J. Glowacki,2005in vitro.]]>180151158Murphy, C.L. and J.M. Polak,2004199451459Sekiya, I., B.L. Larson, J.R. Smith, R. Pochampally, J.G. Cui and D.J. Prockop,200220530541Da Silva Meirelles, L., P.C. Chagastelles and N.B. Nardi,200611922042213Smoak, I.W.,1999in vitro.]]>132733Holland, W., T. Morrison, Y. Chang, N. Wiernsperger and B.J. Stith,20046720812091Zscharnack, M., C. Poesel, J. Galle and A. Bader,20081908193