Journal of Biological Sciences1727-30481812-5719Asian Network for Scientific Information10.3923/jbs.2014.134.141NaveenK.A. SinghS.D. KatariaJ.M. BarathidasanR. DhamaK. 22014142Newcastle Disease (ND) is a highly contagious infection of
poultry which manifest in a wide range of severity from subclinical infection
to lethal disease. In the past, a number of Newcastle disease outbreaks in poultry
and other bird species have been ascribed to pigeon paramyxovirus type-1 (PPMV-1)
infection. The conventional in vivo pathogenicity tests to assess the
pathogenicity of PPMV-1 viruses have provided equivocal results. Lately, restriction
enzyme analysis technique has been employed for unequivocal identification of
individual strains of Newcastle Disease Virus (NDV) in poultry. In this study,
sequence analysis of the F1/F2 cleavage site of the F gene of APMV-1 isolated
from pigeons in India was attempted for pathotype prediction and determination
of molecular epidemiology. Six pigeon origin NDV isolated in India between 1991
and 2001 were selected for this study. A portion of NDV F gene including the
cleavage site was amplified by Polymerase Chain Reaction (PCR) and sequenced
directly. The total number of nucleotide substitution among all six isolates
ranged from 6 to 20; whereas, only four amino acid substitutions were observed.
Nucleotides at position 304 and 357 were unique to all the pigeon isolates.
The cleavage-activation site (380-397) had no nucleotide substitution and all
the six pigeon isolates shared the amino acid sequence 112RRQKRF117
as that of velogenic viruses. The results of this molecular characterization
study of Indian PPMV-1 isolates would help design better prevention and control
measures for this important pathogen.]]>Alexander, D.J.,19971997pp: 541-570pp: 541-570Alexander, D.J.,200142522Alexander, D.J., E.W. Aldous and C.M. Fuller,201241329335Alexander, D.J. and G. Parsons,1984114466469Alexander, D.J., P.H. Russell and M.S. Collins,1984114444446Alexander, D.J., R.J. Manvell, P.A. Kemp, G. Parsons and M.S. Collins et al.,198716553565Cattoli, G., L. Susta, C. Terregino and C. Brown,201123637656Collins, M.S., J.B. Bashiruddin and D.J. Alexander,1993128363370Collins, M.S., I. Strong and D.J. Alexander,1994134403411Collins, M.S., I. Strong and D.J. Alexander,1996141635647Deb, R., S. Chakraborty, B. Veeregowda, A.K. Verma, R. Tiwari and K. Dhama,201345062Dhama, K., M. Mahendran, P.K. Gupta and A. Rai,200832341356Dhama, K., P.M. Sawant, D. Kumar and R. Kumar,201163240Dhama, K., A.K. Verma, R. Tiwari, S. Chakraborty and K. Vora et al.,201311424Dhama, K., K. Karthik, S. Chakraborty, R. Tiwari, S. Kapoor, A. Kumar and P. Thomas,201417151166Dhama, K., M.Y. Wani, R. Deb, K. Karthik and R. Tiwari et al.,20132013Dhama, K., S. Chakraborty, Mahima, M.Y. Wani and A.K. Verma et al.,201316101111Dhama, K., S. Chakraborty, M.Y. Wani, R. Tiwari and R. Barathidasan,20133195208Dhama, K., S. Chakraborty, S. Kapoor, R. Tiwari and A. Kumar et al.,20131513Kataria, J.M., K. Dhama, S. Rahul, S.D. Singh and R.L. Sah,2004255356Kataria, J.M., C.M. Mohan, S. Dey, B.B. Dash and K. Dhama,200575555567Kataria, J.M., K. Dhama, S. Dey and M.C. Madhan,200611618Kant, A., G. Koch, D. Van Roozelaar, F. Balk and A.T. Huurne,199726837849King, D.J.,1996Avian paramyxovirus type 1 from pigeon: Isolate characterization and pathogenicity after chicken or embryo passage of selected isolates.]]>40707714Kommers, G.D., D.J. King, B.S. Seal and C.C. Brown,200145906921Kumanan, K., B. Mathivanan, K. Vijayarani, A. Arul Gandhi, K. Ramadass and K. Nachimuthu,200549105109Mahima, A. Rahal, R. Deb, S.K. Latheef and H.A. Samad et al.,201215754774Mangat, A.P., G. Singh and B.S. Gill,19881988McGinnes, L.W. and T.G. Morrison,19865343356Meulemans, G., M. Gonze, M.C. Carlier, P. Petit, A. Burny and L. Long,1987925562Meulemans, G., T.P. van den Berg, M. Decaesstecker and M. Boschmans,200231515519Miller, P.J., E.L. Decanini and C.L. Afonso,2010102635Mohanty, S.K., J.M. Kataria, S. Mishra, K. Dhama, B.B. Dash and K.C. Verma,200122109112Nanthakumar, T., R.S. Kataria, A.K. Tiwari, G. Buchaiah and J.M. Kataria,200024275286Nanthakumar, T., A.K. Tiwari, R.S. Kataria, G. Butchaiah, J.M. Kataria and P.P. Goswami,200029603607Naveen, K.A. and S.D. Singh,200428150150Naveen, K.A., S.D. Singh and T.R. Mohanty,2009Paramyxovirus type-I.]]>335861Naveen, K.A., S.D. Singh, J.M. Kataria, R. Barathidasan and K. Dhama,20134512311236Oberdorfer, A. and O. Werner,199827237243Pearson, G.L. and M.K. McCann,1975167610614Pearson, J.E., D.A. Senne, D.J. Alexander, W.D. Taylor, L.A. Peterson and P.H. Russel,1987Avian paramyxovirus-1) isolated from pigeons.]]>31105111Roy, P., A.T. Venugopal and A. Koteeswaran,200032183188Seal, B.S., D.J. King and J.D. Bennett,19953326242630Senthilkumar, N., J.M. Kataria, K. Dhama and R. Toroghi,200324191193Singh, G., A.P.S. Mangat and B.S. Gill,198958154158Singh, G., B.S. Gill and M.S. Kwatra,1991Avian paramyxovirus type-1 virus disease in pigeons: A preliminary report.]]>61154155Stewart, G.H.,1971Columbia livia).]]>89225226Sulochana, S. and E.S. Mathew,199172160162Tiwari, R., S. Chakraborty, K. Dhama, M.Y. Wani, A. Kumar and S. Kapoor,20132013Toyoda, T., T. Sakaguchi, K. Imai, N.M. Inocencio, B. Gotoh, M. Hamaguchi and Y. Nagai,1987158242247Utterbak, W.W. and J.H. Schwartz,197316310801088Yang, C.Y., H.K. Shieh, Y.L. Lin and P.C. Chang,199943125130