Journal of Biological Sciences1727-30481812-5719Asian Network for Scientific Information10.3923/jbs.2013.422.426NaghaviNafiseh SadatGolgoljamMahdieh AkbariMojgan 52013135Bacteriophages have been proposed as specific alternative for chemotherapy against multidrug resistant bacterial infections. The aim of the present study was the isolation and purification of bacteriophages from sewage, which were effective against multidrug resistant bacterial isolates and detection of their morphological characteristics. Sewage and clinical samples were used for the isolation of antibiotic resistant pathogenic bacteria. The bacteriophages were isolated and purified from sewage samples by overlay cultures of the isolated bacteria after centrifugation and filtration procedures. Three isolates of the most multidrug resistant Escherichia coli, Klebsiella pneumonia and Pseudomonas aeruginosa selected for bacteriophage isolation. Most of the isolates were resistant to different classes of antibiotics in the aminoglycosides, cephalosporins, macrolides, tetracyclines and β-lactam antibiotic groups. The isolated bacteriophges showed different plaque morphologies on the bacterial cultures. The three purified bacteriophages had icosahedral or hexagonal heads. Long tails observed in Escherichia coli bacteriophages, with the total length of about 170 nm for the phages particles; short tails observed in Klebsiella pneumonia phages particles, with the approximate total lengths of 150 nm; and short tails with the approximate 35 nm total particles length observed in Pseudomonas aeruginosa bacteriophages.]]>Begum, Y.A., S. Chakraborty, A. Chowdhury, A.N. Ghosh and G.B. Nair et al.,2010Escherichia coli.]]>59266272Boule, J., P.L. Sholberg, S.M. Lehman, D.T. Ogorman and A.M. Svircev,2011Erwinia amylovora and their potential as biological control agents in British Columbia, Canada.]]>33308317Brooks, G.F., J.S. Butel and S.A. Morse,201024th Edn.,Chan, B.K. and S.T. Abedon,201278123Chhibber, S., S. Kaur and S. Kumari,2008Klebsiella pneumoniae B5055-mediated lobar pneumonia in mice.]]>5715081513 Foddai, A., C.T. Elliott and I.R. Grant,2009Mycobacterium avium subsp. paratuberculosis cells.]]>7538963902Garbe, J., B. Bunk, M. Rohde and M. Schobert,2011Pseudomonas aeruginosa phage JG004.]]>2011Hershey, A.D. and W. Dove,19831983pp: 3-20pp: 3-20Knipe, D.M., P.M. Howley and D.E. Griffin,20014th Edn.,Kochetkova, V.A., A.S. Mamontov, R.L. Moskovtseva, E.I. Erastova, E.I. Trofimov, M.I. Popov and S.K. Dzhubalieva,198962326Mahon, M.R., D.C. Lehman and G. Manuselis,20104th Edn.,Matsuzaki, S., M. Yasuda, H. Nishikawa, M. Kuroda and T. Ujihara et al.,2003Staphylococcus aureus infection by novel bacteriophage phi MR11.]]>187613624Meladze, G.D., M.G. Mebuke, N.S. Chkhetia, N.I. Kiknadze and G.G. Koguashvili et al.,198215356Sambrook, J. and D.W. Russell,20013rd Edn.,Sulakvelidze, A., Z. Alavidze and J.G. Morris Jr.,200145649659Wills, Q.F., C. Kerrigan and J.S. Soothill,2005Staphylococcus aureus abscesses in a rabbit model.]]>4912201221