Journal of Biological Sciences1727-30481812-5719Asian Network for Scientific Information10.3923/jbs.2013.283.287Sargasam wightii Greville]]>SaravananR. EbenezarK. Kumar RajasekaranS. ThamaraiselvanR. 42013134The aim of the present study is to isolate the sulfated polysaccharides from marine macro algae S. wightii. The crude sulfated polysaccharides were fractionated by anionic resin (Amberlite IRA-900) and the separated active fractions were confirmed by agarose gel electrophoresis. The active fractions were pooled, dialyzed and purified by molecular sieve (Sephadex G-100) chromatography. The molecular weight of fractionated as well as purified sulfated polysaccharides was determined through gradient polyacrylamide gel electrophoresis (PAGE) and the disaccharide profile of purified sulfated polysaccharides was also analyzed. The structure of purified sulfated polysaccharides was dogged by 1H-NMR spectrum. The elements such as Carbon, Hydrogen and Nitrogen (CHN) and Activated Partial Thromboplastin Time (APTT) were observed from the purified sulfated polysaccharides. The hexosamine, uronic acid and the CHN content in the purified sulfated polysaccharides were found to be low (except molecular weight) when compared to standard heparan sulfate. The molecular weight of purified sulfated polysaccharides have 6,500 Da and the concentration of disaccharide profile such as 5.52% of uronic acid, 3.47% hexosamine and 5.4% of sulfate. APTT of fractionated and purified sulfated polysaccharides was found to be 24.6 and 31.1 IU mg-1, respectively. These results suggest that the sulfated polysaccharides from S. wightii could be an alternative source of anticoagulant.]]>Bitter, T. and H.M. Muir,19624300334Cassaro, C.M. and C.P. Dietrich,197725222542261Edens, R.E., A. Al-Hakim, J.M. Weiler, D.G. Rethwisch, J. Fareed and R.J. Linhardt,199281823827Ibtissam, C., R. Hassane, M.J. Jose, D.S.J. Francisco, G.V.J. Antonio, B. Hassan and K. Mohamed,2009812581262Larsen, B., A. Haug and T.J. Painter,199620219230Leite, E.L., M.G.L. Medeiros, H.A.O. Rocha, G.G.M. Farias and L.F. da Silva et al.,1998Spatoglossum schroederi.]]>132215228Mao, W., X. Zang, Y. Li and H. Zhang,2006Ulva conglobata and their anticoagulant activity.]]>18914Medcalf, E.G. and B. Larsen,1977Ascophyllum nodosum and Fucus vesiculosus.]]>59531537Mishra, V.K., F. Temelli, B. Ooraikul, P.F. Shacklock and J.S. Craigie,1993Palmaria palmate.]]>36169174Nardella, A., F. Chaubet, C. Boisson-Vidal, C. Blondin, P. Durand and J. Jozefonvicz,1996Ascophyllum nodosum.]]>289201208Painter, T.J.,19831983pp: 195-285pp: 195-285Pugazhendi, T.,2002Acanthopora spicifera (Vahl.) Boegesen.]]>2002Saravanan, R. and A. Shanmugam,2010Amussium pleuronectus (Linne) using chromatography.]]>160791799Saravanan, R. and A. Shanmugam,2011Amussium pleuronectus (Linne.) an alternative source of heparin?]]>8610821084Saravanan, R., S. Sambasivam, A. Shanmugam, D.S. Kumar, T.T. Vanan and R.A. Nazeer,2009Conus figulinus Linneaus (1758).]]>8266271Smit, A.J.,200416245262Sudo, M., K. Sato, A. Chaidedgumjorn, H. Toyoda, T. Toida and T. Imanari,20011H nuclear magnetic resonance spectroscopic analysis for determination of glucuronic and iduronic acids in dermatan sulfate, heparin and heparan sulfate.]]>2974251Terho, T. and K. Hartiala,197141471776Toida, T., H. Yoshida, H. Toyoda, I. Koshiishi, T. Imanari, R.E. Hileman, J.R. Froman and R.J. Linhardt,1997322499506Wagner, W.D.,197994394398Yasuda, S., T. Atsumi, M. Leko and T. Koike,20042-glycoprotein I, anti-β2-glycoprotein I and fibrinolysis.]]>114461465