Journal of Biological Sciences1727-30481812-5719Asian Network for Scientific Information10.3923/jbs.2013.131.138Phellinus linteus)]]>SrivilaiPrayook ChaiseanaWasana LoutchanwootPanida DornbunditPiyarat 32013133Cambodian Phellinus linteus is a white rot fungus that
behaves as a plant pathogen. This fungus was first used for studying changes
in the wood chemistry and structure of Shorea (Shorea obtusa). Cambodian
P. linteus comprises two different types of mycelia, namely monokaryon
(n) and dikaryon (2n). Wood blocks of Shorea were exposed to monokaryons
and dikaryons under in vitro condition and biodegradation took place
over a 12-week-period. Shorea wood block degradation was determined by
(1) wood weight loss (2) Fourier Transform Infrared (FTIR) and (3) Scanning
Electron Microscopy (SEM). Results showed that the degradation of Shorea
wood blocks by monokaryons and dikaryons was 57.48 and 55.73%, respectively.
The FTIR analyses showed that the Shorea wood blocks degraded by the
monokaryons and dikaryons were differed in their chemical components (aromatic,
C-H and C-O). The results also suggested that the lignin and carbohydrates were
decayed. Meanwhile, the C-O, C-O-H and C-H groups were decreased, revealing
that hemicelluloses and cellulose polymers were broken down by hydrolytic enzymes
during fungal growth. SEM was used to observe the physical changes of the Shorea
wood blocks and it showed that the wood cells were changed. In conclusion, Cambodian
P. linteus monokaryons are more appropriated for the paper industry
(biopulping and bio-breaching) and enzyme technology than dikaryons, due to
monokaryons leave no dark pigments and residues from the fruiting bodies. Moreover,
monokaryons have a simple genetic system (n) for genetic and biochemical analyses.]]>Addleman, K. and F. Archibald,1993Trametes versicolor.]]>59266273Akio, E., T. Hiromi and F. Goro,1988428593Berben, S.A., J.P. Rademacher, L.O. Sell and D.B. Easty,198770129140Blanchette, R.A., L. Otjen, M.J. Effland and W.E. Eslyn,1985193546Bjarmestad, S. and O. Dahlman,20027458515858Breen, A. and F.L. Singleton,199910252258Boerjan, W., J. Ralph and M. Baucher,200354519546Fackler, K., M. Schwanninger, C. Gradinge, B. Hinterstoisser and K. Messne,2007271162169Faix, O.,19921992pp: 83-132pp: 83-132Faix, O., J. Bremer, O. Schmidt and S.J. Tatjana,199321147162Fischer, M and M. Binder,2004Phellinus s.l.]]>96799811Friese, M.A and S. Banerjee,199246246248Ferraz, A., J. Baeza, J. Rodriguez and J. Freer,200074201212Genestar, C. and J. Palou,2006384987993Gierlinger, N., D. Jacques, M. Schwanninger, R. Wimmer and L.E. Paques,2004Larix sp.) and relationships to brown-rot decay-resistance.]]>18230236Kacurakova, M., P. Capek, V. Sasinkova, N. Wellner and A Ebringerova,200043195203Kothe, E.,200156602612Kues, U., 2000Coprinus cinereus.]]>64316353Pandey, K.K.,19997119691975Parker, F.S.,1983pp: 21-25pp: 21-25Pandey, K.K. and A.J. Pitman,200352151160Pandey, K.K. and A.J. Pitman,20044223402346Pasikatan, M.C., J.L. Steele, C.K. Spillman and E. Haque,20019153164Raberg, U. and G. Daniel,200963906912Rana, R., R. Langgenfeld-Heyser, R. Finkeldey and A. Polle,201044225242Rodrigues, J., O. Faix and H. Pereira,1998Eucalytus globules wood using FTIR spectrometry.]]>524650Schultz, T.P. and W.G. Glasser,1986403444Schwanninger, M., J. Rodrigues, H. Pereira abnd B. Hinterstoisser,2004362340Srivilai, P.,20062006Worrall, J.J., S.E. Anagnost and R.A. Zabel,1997Lignicolous fungi.]]>89199219