Journal of Biological Sciences1727-30481812-5719Asian Network for Scientific Information10.3923/jbs.2013.10.17Isochrysis sp.) in Laboratory Cultured Conditions]]>JalalK.C.A. ShamsuddinA.A. RahmanM.F. NurzatulN.Z. RozihanM. 12013131A study was conducted to determine the total content of carotenoids,
in Isochrysis sp. under stress condition (nitrogen starvation). Isochrysis
sp. was cultured in f/2 medium at optimum light intensity (1200 Lux) with constant
aeration (4.5 L min-1) at 8 pH. Salinity of the water was maintained
between 20-24 g L-1 and temperature range was 20-24°C. The culture
of Isochrysis sp. showed a short lag phase lasting about 24 h followed
by exponential phase starting from day 2 until day 10. In day 4, specific growth
rate (μ) for Isochrysis sp. was 1.6059 day-1, the highest
rate compared to other day. Stress parameter (NO3¯ starvation)
was introduced into the mass culture on day 4 by eliminating NO3¯
in f/2 media. There was considerable declining (p<0.05) in cell count was
observed in the absence of nitrogen proving the dependency of carotenoid production
over the nitrogen limitation which probably due to the main role played by the
nitrogen in various metabolic activities of the cell. The highest total carotenoid
per cell was recorded at day 10 with 0.001932347 mg mL-1, whereas
the lowest total carotenoid content per cell was observed at day 4 with 0.000100649
mg mL-1. The finding reveals that it is best to harvest the carotenoid
on day 10, when the maximum carotenoid can be obtained.]]>Abd El-Baky, H.H., F.K. El-Baz and G.S. El-Baroty,20032222240Ausich, R.L.,19976921692173Bone, R.A., J.T. Landrum, Z. Dixon, Y. Chen and C.M. Llerena,200071239245Connor, W.E. and M. Neuringer,198881741De Pauw, N. and G. Persoone,19881245256Moreau, D., C. Tomasoni, C. Jacquot, R. Kaas and R. Le Guedes et al.,2006Odontella aurita as potent anti-proliferative agents in bronchopulmonary and epithelial cell lines.]]>2297103Enright, C.T., G.F. Newkirk, J.S. Craigie and J.D. Castell,1986Ostrea edulis L.]]>96113Eskling, M., P.O. Arvidsson and H.E. Akerlund,1997100806816Green, J.C. and R.N. Pienaar,1977Isochrysis Parke, Dictateria Parke and Imantonia Reynolds.]]>5717Guillard, R.R. and J.H. Ryther,19628229239Hagen, C., W. Braune, K. Vogel and D.P. Hader,1993Haematococcus lacustris (Girod) Rostafinski (Volvocales). V. Influences on photomovement.]]>16991995Hori, T. and J.C. Green,1991Isochrysis galbana (Prymnesiophyta).]]>71137152Hughes, D.A., A.J. Wright, P.M. Finglas, A.C. Polley, A.L. Bailey, S.B. Astley and S.B. Southon,20001S1S5Kaplan, D., Z. Cohen and A. Abeliovich,1985Isochrysis galbana.]]>108213217Khachik, F., R.F. Beecher, J.T. Vanderslice and G. Furrow,19986807811Kjell, I.R., R. Jose and O. Yngvar,199430972979Koven, W.M., G.W. Kissil and A. Tandler,198979185191Lapointe, B.E.,1993Gracillaria tikvdhlae (Rhodophyceae) in the florida keys as an experimental field study.]]>93561568Lichtenthaler, H.K.,1987148350382Lopez-Ruız, J.L., R. Garcia and M.S. Ferreiro-Almeda,1995Chaetoceros gracilis with zeolitic product zeestec-56 and a commercial fertilizer as a nutrient.]]>14367372Machlin, L.J.,1995354150Parke, M.,194928255286Ramamurthy, V.D. and S. Krishnamurthy,2003Trichodesmium erythraeum.]]>654348Renaud, S.M. L.V. Thinh and D.L. Parry,1999170147159Richmond, A.,200012441451Sandmann, G.,200161417Sukenik, A. and R. Wahnon,1991Isochrysis galbana.]]>976172Suzuki, R. and T. Ishimaru,199046190194Suzuki, R., M. Takahashi, K. Furuya and T. Ishimaru,199349571580Taylor, C.B.,1996814471450Van den Berg, H., R. Faulks, H.F. Granado, J. Hirschberg and B. Olmedilla et al.,200080880912Volkman, J.K., M.R. Brown, G.A. Dunstan and S.W. Jeffrey,1993296778Webb, K.L. and F.E. Chu,198324123128Wellburn, A.R.,1994144307313Whyte, J.N.C.,198760231241Yongmanitchai, W. and O.P. Ward,19923029632967Zapata, M. and J.L. Garrido,199131589594Zhu, C.J., Y.K. Lee and T.M. Chao,1997Isochrysis galbana TK1.]]>9451457