Journal of Biological Sciences1727-30481812-5719Asian Network for Scientific Information10.3923/jbs.2012.239.245PhetdeeK. RattanamaneeK. TeaktongT. ViyochJ. 42012124The traditional use of tamarind and its health benefits while
the high phenolic content of its seed coat suggest it might be explored as a
skin lightener. For this, we focused on the possible role of the seed coat extract
in inhibiting melanogenesis. The seed coat of tamarind (Tamarindus indica
L.) was extracted with ethyl acetate, which among other solvents recovered highest
phenolic content (85.6±0.9 mg g-1 catechin equivalents-Folin-Ciocalteu
assay). B16-F1 melanoma cells were stimulated by α-melanocyte stimulating
hormone (α-MSH) for 48 h and the extract added after the first 24 h, it
dose-dependently inhibited melanin production by 20-32%. When MSH was added
24 h after the extract, the melanin reduction was about 42-59%. Kojic acid (50
μg mL-1), a well-known hypopigmenting agent had similar effects.
Cell viability and morphology were unaffected by any of the concentration used.
The extract also inhibited tyrosinase activity (IC50 = 152.1±10.2
μg mL-1). This is the first report shows that tamarind seed
coat can inhibit melanogenesis and suggests that further refinements may show
their benefit in hyperpigmentation improvement.]]>Bohm, S.K., W. Kong, D. Bromme, S.P. Smeekens and D.C. Anderson et al.,199631410091016Bravo, L.,199856317333Cherniack, E.P.,201017181187Cos, P., L. Ying, M. Calomme, J.P. Hu and K. Cimanga et al.,1998617176Donsing, P., N. Limpeanchob and J. Viyoch,2008594158Hensley, K. and R.A. Floyd,2002397377383Huang, K.F., Y.W. Chen, C.T. Chang and S.T. Chou,2005Graptopetalum paraguayense E. Walther extracts on mushroom tyrosinase.]]>89583587Kim, Y.J, H. Uyama and S. Kobayashi,2004320256261Momtaz, S., B.M. Mapunya, P.J. Houghton, C. Edgerly, A. Hussein, S. Naidoo and N. Lall,2008Sideroxylon inerme L. stem bark, used in South Africa for skin lightening.]]>119507512Morandini, R., J.M. Boeynaems, S.J. Hedley, S. MacNeil and G. Ghanem,1998175276282Mun, Y.J., S.W. Lee, H.W. Jeong, K.G. Lee, J.H. Kim and W.H. Woo,200427806809Paine, C., E. Sharlow, F. Liebel, M. Eisinger, S. Shapiro and M. Seiberg,2001116587595Pumthong, G.,1999Tamarindus indica Linn.]]>1999Seiberg, M., C. Paine, E. Sharlow, P. Andrade-Gordon, M. Costanzo, M. Eisinger and S.S. Shapiro,2000115162167Seiberg, M., C. Paine, E. Sharlow, P. Andrade-Gordon, M. Costanzo, M. Eisinger and S.S. Shapiro,20002542532Siddhuraju, P.,2007Tamarindus indica seed coat.]]>40982990Siegrist, W. and A.N. Eberle,1986In situ melanin assay for MSH using mouse B16 melanoma cells in culture.]]>159191197Singleton, V.L., R. Orthofer and R.M. Lamuela-Raventos,19991999pp: 152-178pp: 152-178Soong, Y.Y. and P.J. Barlow,200488411417Sudjaroen, Y., R. Haubner, G. Wurtele, W.E. Hull and G. Erben et al.,2005Tamarindus indica L.) seeds and pericarp.]]>4316731682Yoon, K.N., N. Alam, K.R. Lee, P.G. Shin, J.C. Cheong, Y.B. Yoo and T.S. Lee,2011Lentinus lepideus.]]>1623342347